Hyuk Jun Lee

Effects of microbial additives on chemical composition and fermentation characteristics of barley silage.

This study examined the effects of bacterial inoculants on chemical composition and fermentation indices of barley silage. Barley forage (Youngyang) was harvested at 24% dry matter (DM) and wilted to 47.9% DM. The wilted barley forage was chopped to 3–5 cm length and applied with no inoculant (CON), L. plantarum (1×1010 cfu/g, LP) or Effective Microorganisms (0.5×109 cfu/g, EM). Then the forages were ensiled in four replications for each treatment in 20 L mini silos and stored for 100 days. The contents of crude protein and ether extract were higher in CON silage ensiled for 100-d, while the contents of DM and crude ash were higher in EM silage (p<0.05). The contents of ADF, NDF and hemicellulose as well as the in vitro DM digestibility were not affected by microbial inoculation (p>0.05). The pH, ammonia-N concentration and lactate to acetate ratio were higher (p<0.05) in CON silage, while lactate concentrations were higher (p<0.05) in CON and LP silage. Acetate concentration and lactic acid bacteria was increased (p<0.05) by both inoculants (LP and EM), but propionate concentration and yeast was increased (p<0.05) by EM and LP, respectively. These results indicated that the fermentation quality of barley silage was improved by the application of bacterial inoculants.

Effect of Microbial and Chemical Combo Additives on Nutritive Value and Fermentation Characteristic of Whole Crop Barley Silage

This study was conducted to assess the effects of microbial and chemical combo additives on nutritive values, fermentation indices and aerobic stability of whole crop barley silage. Barley forage (Youngyang) was harvested at about 30% dry matter (DM) by treatments, chopped to 5 cm length and treated with distilled water only (CON), Lactobacillus plantarum (INO), propionic acid (PRO) or an equal mixture of INO and PRO (MIX). Barley forages were ensiled in 4 replications for 0, 2, 7, and 100 days. On 100 days of ensiling, MIX silage had higher (p<0.05) in vitro DM digestibility than CON silage, but lower (p<0.05) acid detergent fiber concentration. The pH in all treated silages was lower (p<0.05) than CON silage. The MIX silage had higher (p<0.05) lactate concentration and lactate to acetate ratio than in CON, but lower (p<0.05) yeast count. Aerobic stability in CON, PRO, and MIX silages were higher (p<0.05) than in INO silage. It is concluded that microbial and chemical combo additives using L. plantarum and propionic acid could efficiently improve nutritive values of barley silage in terms of increased in vitro DM digestibility compared to other treatments. In addition, all treatments except CON reduced yeast count which is the initiate microorganism of aerobic spoilage.

Effects of fermented persimmon extract supplements on chemical composition and fermentation characteristics of barley silage

This study was conducted to estimate the effect of fermented persimmon extract (FPE) supplementation levels on chemical composition and fermentation characteristics of barley silage. Barley forage was harvested at 25% dry matter and chopped to 5 cm lengths. The FPE was applied at 0, 0.4, 0.8 and 1.6 g/kg of barley forage (fresh weight basis). After sub-sampling of barley forages (0 day), a total of 64 laboratory mini silos (5 kg capacity) were ensiled to generate quadruplicate data from each of four treatments for four ensiling durations (2, 6, 40 and 120 days). The chemical compositions and in vitro digestibility of barley forage (0 day) were not affected by FPE supplementation. However, the content (L, P = 0.035) and in vitro digestibility (L, P = 0.041) of neutral detergent fiber on 120-day barley silage decreased by increasing FPE supplements. Acetate content of barley silage ensiled for 120 days increased (Q, P = 0.004) by increasing FPE supplements, whereas pH (L, P < 0.001), lactate content (Q, P < 0.001) and lactate-to-acetate ratio (Q, P < 0.001) decreased. These results indicate that addition of FPE can increase the acetate content which has potential to increase aerobic stability.

Nutritional characterization of tannin rich chestnut (Castanea) and its meal for pig

This study was conducted to estimate the nutritional values of different parts of chestnut and the effects of chestnut meal on feed intake, nutrient digestibility and blood metabolites in pig. Nutritionally, chestnut kernel was found as the best considering the highest crude protein and energy content, highest dry matter and protein digestibility and absence of tannin in it. In the animal experiment, 16 pigs were selected and randomly allocated in one of four dietary treatments in which chestnut meal replaced 0, 5, 10 and 15% of dry matter with basal diet, and housed individually into a metabolic cage for 21 days. Dry matter and crude protein intakes were highest (p < 0.05) in 10% supplementation. Digestibility of dry matter, crude protein, ether extract, crude ash and tannin decreased linearly (p < 0.05) with increasing chestnut meal supplementation. Considering feed intake and digestibility, supplementation of chestnut meal at 5% for pig could be suggested.

Effects of Bacterial Inoculants and Cutting Height on Fermentation Quality of Barley Silage

ABSTRACT This study was conducted to investigate the effects of bacterial inoculation (Lactobacillus plantarum) and cutting height on the chemical composition, fermentation characteristics and in vitro dry matter digestibility (IVDMD) in whole crop barley silage. Barley forage (Youngyang hybrid) was harvested at about 27% of dry matter (DM) level at two different cutting height (5 vs. 15 cm). And it was chopped to 5 cm length and treated with or without L. plantarum. Four replicates of each treatment were ensiled into 10 L mini silo (3 kg) for 100 days. After 100 days, bacterial inoculation decreased(p=0.001) DM content, while increased cutting height increased (p=0.002) DM in uninoculated silage. Crude protein (CP) concentration was decreased by increasing height in uninoculated silage (8.84 vs. 8.16) but increased in inoculated silage (8.19 vs. 8.99). Both neutral detergent fiber (NDF) (p<0.011) and acid detergent fiber (ADF) (p<0.004) were decreased by increasing cutting height of forage at harvest. The IVDMD and ammonia-N was increased (p=0.001) by increasing cutting height and inoculation, respectively. Lactic acid bacteria (LAB) was increased(p=0.002) in inoculated silage, but yeast count was decreased (p=0.026) in uninoculated silages. It is concluded that increased cutting height of forage at harvest could be useful to make a fibrous portion with increase of dry matter digestibility of silages.(

Effects of dietary n‐6/n‐3 fatty acid ratio on nutrient digestibility and blood metabolites of Hanwoo heifers

The objectives of this study were to investigate the effects of dietary n-6/n-3 fatty acid (FA) ratio on digestibility, blood metabolites and FA profile of Hanwoo heifers. Fifteen Hanwoo heifers (22 ± 3 months old; 357 ± 69.7 kg) were randomly assigned to three dietary treatments with n-6/n-3 FA ratios of 2.07, 5.18 and 7.37. The animals were housed individually in digestion crates and fed total mixed rations at 2.2% of body weight for 2 weeks of adaptation and 1 week of collection. Treatment effects on in vivo digestibility, plasma metabolite and fatty acid profiles, and in vitro ruminal fermentation and fatty acid profiles were examined. In vivo digestibility was not affected (P > 0.05) by dietary n-6/n-3 FA ratio. However, in vitro dry matter digestibility and concentrations of total volatile fatty acids and propionate decreased (P < 0.05) linearly with increasing n-6/n-3 FA ratio. Plasma insulin and progesterone increased linearly (P < 0.05), but linolenic acid and total n-3 FA decreased linearly (P < 0.05) with increasing n-6/n-3 ratio. Increasing the dietary n-6/n-3 FA ratio can increase the n-6/n-3 FA ratio in plasma and ruminal fluid as well as plasma progesterone secretion.

Growth Performance and Fatty Acid Profiles of Korean Native Chicken Fed Diets with Mixed Probiotics

Abstract This study aimed to investigate the effects of dietary supplementation of mixed probiotics on growth performance and fatty acid profiles of Korean native chicken and to provide information regarding producers. Ninety six Korean native chicken (48 males and 48 females, Hanhyup-3-ho) were allocated to 16 wire cages with 4 treatments, 4 replicates and 6 chicks (3 males and 3 females) per wire cage and fed one of four diets containing 0, 0.5, 1 and 1.5% mixed probiotics for 6 weeks. There were no differences among treatments in growth performance of Korean native chicken. For fatty acid profiles, no statistically differences in each fatty acid, total saturated fatty acid and unsaturated fatty acids were observed in all treatments. In the present study, irrespective of statistically differences, inclusion of mixed probiotics tended to improve growth performance, reduce saturated fatty acid and increase unsaturated fatty acids in breast muscles from Korean native chicken compared to controls.

Effects of Manure Additives on pH and Pathogen Populations in Hanwoo (Korean native cattle) Manure

Abstract The objective of this study was to evaluate the effect of applying alum (aluminum sulfate) and aluminum chloride on pH and pathogen populations of Hanwoo manure. A total of 36 steers (8 months old and averaging 300 kg in weight) were used in this trial and allotted to 9 pens (3 replication pens per group with 4 steers per experimental unit, 5 x 8 m). Chemical additives were applied as a top dressing with garden rake to a depth of 1 cm of manure with wood shavings in each treatment. The chemical amendments were control (without chemical amendments), 50 g of alum and 50 g of aluminum chloride/kg of Hanwoo manure. The experiment was carried out for 4 weeks. Adding alum and aluminum chloride to Hanwoo manure reduced ( P < 0.05 ) pH compared to untreated controls during the 4-wk period. Both levels of the alum and aluminum chloride treatments tested decreased ( P < 0.05 ) Escherichia coli and Salmonella enterica populations in Hanwoo manure at 2 and 4 weeks. It appears that the reduction in pathogen populations was primarily associated with the lower manure pH. If more strict environmental regulations are put into effect regarding pathogen populations from Hanwoo facilities, treating Hanwoo manure with alum and aluminum chloride may be a good management practice.

Effect of microbial inoculants on fermentation quality and aerobic stability of sweet potato vine silage

Objective This study was conducted to evaluate the effect of homo or hetero fermentative inoculants on fermentation quality and aerobic stability of sweet potato vine (SPV) silage containing Italian ryegrass hay as moisture absorbent. Methods The SPV was harvested at 15% dry matter, mixed with Italian ryegrass hay at 1:1 ratio on a fresh weight basis, and chopped to 3 to 5 cm length. After then, the chopped forage mixture was ensiled into 20-L mini silos in quadruplicate for 7, 48, and 100 days after application of microbial inoculants at 1.2×105 colony forming units (cfu)/g of forage following: no inoculant (CON), Lactobacillus plantarum as a homo fermentative (LP), Lactobacillus buchneri as a hetero fermentative (LB), and mixture of LP and LB at 1:1 ratio as a combo fermentative (MIX). Results The LP and MIX silages had lowest pH (p<0.001) on 7 and 48 days, while MIX and CON silages had greatest lactate concentrations (p<0.05) on 7 and 48 days, respectively. Acetate concentrations were highest (p<0.01) in LB and MIX silages on 7 days, and in LB silage on 48 days, while lactate to acetate ratios were lowest (p<0.001) in LB silages. The chemical compositions and nutrient digestibility of silage ensiled for 100 days was not affected by inoculants. On 100 days of ensiling, LB silage had lowest (p<0.01) lactate concentration and lactate to acetate ratio, but highest acetate concentration. Aerobic stability was highest (p<0.001) in LB silage followed in MIX silage. On contrast, LB silage had lowest (p<0.05) lactic acid bacteria and mold. Conclusion The results indicated that application of LB solely had a better effect on aerobic stability than not only LP, but also MIX. However, LP application did not show beneficial effects from the viewpoints of fermentation quality and aerobic stability compared to CON.

Effects of Different Cutting Height on Nutritional Quality of Whole Crop Barley Silage and Feed Value on Hanwoo Heifers.

The present study evaluated the effects of different cutting height on nutritive value, fermentation quality, in vitro and in vivo digestibility of whole crop barley silage. Whole crop barley forage (Yuyeon hybrid) was harvested at height of 5, 10, and 15 cm from the ground level. Each cutting height was rolled to make round bale and ensiled for 100 days. After 100 days of ensiling, pH of silage was lower (p<0.05) in 5 cm, but no difference between 10 and 15 cm of cutting height. The content of lactate and lactate to acetate ratio were increased (p<0.05) in 5 cm of cutting height, whereas the acetate content was higher (p<0.05) in 10 and 15 cm than that of 5 cm cutting height. Aerobic stability was greater (p<0.05) in silages of 10 and 15 cm of cutting height. Three total mixed rations (TMR) were formulated with silages from the three different cutting heights (TMR5, TMR10, and TMR15) incorporated as forage at 70:30 ratio with concentrate (dry matter [DM] basis). In vitro dry matter digestibility was higher (p<0.05) in the TMR5 and TMR10 than that in TMR15, whereas in vitro neutral detergent fiber digestibility was higher (p<0.05) in the TMR10 and TMR15 than that in TMR5. Concentration of NH3-N was highest (p<0.05) in the TMR10 followed by TMR15 and TMR5. Total volatile fatty acid was decreased (p<0.05) with increased cutting height. The digestibility of DM and neutral detergent fiber were highest (p<0.05) in TMR15, than those in TMR5 and TMR10, whereas acid detergent fiber digestibility was higher (p<0.05) in TMR5 than that in TMR10. The results showed that increasing cutting height, at least up to 10 to 15 cm, of whole crop barley forage at harvest (Yuyeon) may be beneficial for making silage for TMR formulation and increasing digestibility of DM and NDF.