Hyun-Wook Kim

Electron Tomography and Synapse Study

Electron tomography (ET) is a useful tool to investigate threedimensional (3D) details based on virtual slices of relative thick specimen. A single electron transmission microscopic image is to ET what single X-ray image is to computed X-ray tomography (Rhyu & Park, 2008). As computed X-ray tomographic image shows cross sectional information of the body without real sectioning in hospital, we can get virtual cross sectional image of relative thick specimen up to resolution 2.4 A recently (Scout et al., 2012). Not only technical upgrade of ET, but sophisticated analyses of various specimens are main interest of electron tomographic studies (Frey et al., 2006; Mun et al., 2008; Jou et al., 2013). By using relative thick specimen and very thin virtual slice up to 1 nm, ET is a useful tool dissecting very small structures and relatively large structures at the same time. PRINCIPLES OF ELECTRON TOMOGRAPHY

Morphological Diversity of Mitochondria in Cultured Astrocyte, HeLa, COS7 Cells under High Voltage Electron Microscopy

Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it is closely related to cellular apoptosis and necrosis. Linear, branched, circular, and ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. In this study, we investigated the density and ratio of the each morphological categories of mitochondria in a few normal cultured cells; astrocyte, HeLa and COS7 cells, of which metabolic activities are different, with high voltage electron microscopy. The absolute number and relative number per unit area of mitochondria was largest in astrocyte. But, the proportion of different mitochondrial shape was similar among cells. These results shows the numerical profiles but not morphological profiles of mitochondria are related to the metabolic activity of each cell line.

Different types of multiple-synapse boutons in the cerebellar cortex between physically enriched and ataxic mutant mice

Experience‐dependent synapse remodeling is associated with information storage in the nervous system. Neuronal synapses show alteration in various neurological and cognitive disorders in their structure and function. At the ultrastructural level, parallel fiber boutons contacting multiple spines of Purkinje cells in the cerebellar cortex are commonly observed in physiologically enriched animals as well as pathological ataxic mutants. However, the dendritic origin of those spines on parallel fiber multiple‐synapse boutons (MSBs) has been poorly understood. Here, we investigated this issue by 3‐dimensional ultrastructural analysis to determine synaptic connectivity of MSBs in both mice housed in physically enriched environment and cerebellar ataxic mutants. Our results demonstrated that environmental enrichment selectively induced MSBs to contact spines from the same parent dendrite, indicating focal strengthening of synapse through the simultaneous activation of two adjacent spines. In contrast, ataxic mutants displaying impaired motor coordination had significantly more MSBs involving spines originating from different neighboring dendrites compared to both wild‐type and environmentally enriched animals, suggesting that compromising multiple synapse formation may lead to abnormal motor behavior in the mutant mice. These findings propose that environmental stimulation in normal animals mainly involves the refinement of preexisting synaptic networks, whereas pathological ataxic conditions may results from less‐selective but compromising multiple synaptic formation. This study underscores that different types of multiple synapse boutons may have disparate effects on cerebellar synaptic transmission.

A Study About Grid Impose Method On Real-Time Simulator For Wind-Farm Management System

Owing to the variability of large-scaled wind power system, the development of wind farm management technologies and related compensation methods have been receiving attention. To provide an accurate and reliable output power, certain wind farm adopts a specified management system including a wind prediction model and grid expectation solutions for considering grid condition. Those technologies are focused on improving the reliability and stability issues of wind farms, which can affect not only nearby system devices but also a voltage condition of utility grid. Therefore, to adapt the develop management system, an expectation process about voltage condition of Point of Common Coupling should be integrated in operating system for responding system requirements in real-time basis. This paper introduce a grid imposing method for a real-time based wind farm management system. The expected power can be transferred to the power flow section and the required quantity about reactive power can be calculated through the proposed system. For the verification process, the gauss-seidel method is introduced in the Matlab/Simulink for analysing power flow condition. The entire simulation process was designed to interwork with PSCAD for verifying real power system condition.

Analysis of Skin Micro-relief on Healthy Korean Youth According to Sex

The skin is the largest organ of the integument system whose surface is closely related to many physiological and pathological conditions. The skin surface is closely related to functional status of the epidermis and dermis. The outermost surface of the skin shows characteristic pattern defined by small furrows are called micro-relief. The micro-relief change is according to physiological and pathological conditions of the skin. But, there is no data on health Korean skin micro-relief yet. This study presents some aspect of health Korean skin micro-relief from the twenties. The silicon replicas obtained from forearm, dorsum hand and finger were analyzed by profiles were accessed by stereoscopy. We measured density of furrow, width of furrow, and depth of furrow in micro-relief with sectioned silicon replica sample using stereoscope. Stereoscopic analysis showed that male group has a larger density of furrow and depth furrow in finger significantly. The densities of furrow were shown different not only between forearm and finger but also between finger and hand in male with female group. In conclusion, there were differences results between sex different and regional difference in skin replica profile. These quantitative data can be used for basis of further skin research for Korean.