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Dive into the research topics where Hyung Joon Cha is active.

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Featured researches published by Hyung Joon Cha.


Biotechnology Progress | 2008

Carassius auratus-originated recombinant histone H1 C-terminal peptide as gene delivery material.

Hee Jung Jung; Dong Soo Hwang; Quan De Wei; Hyung Joon Cha

The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research. Protein‐based gene delivery including histone proteins has recently emerged as a powerful technique for non‐viral DNA transfer. Histones are DNA‐binding proteins that function in DNA packaging and protection. In particular, histone H1 is largely responsible for the stabilization of higher‐order chromatin structures. Several studies have examined the use of full‐length histone H1‐mediated gene transfer, and a few studies have investigated the use of C‐terminal histone H1 fragments as gene‐transfer materials. Previously, we cloned a novel histone H1 cDNA from the goldfish Carassius auratus and found that a recombinant histone H1 C‐terminal short peptide (H1C) of 61 amino acids has comparable DNA binding and protection functions as full‐length histone H1. In the present work, we successfully expressed and purified soluble recombinant H1C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. We confirmed its DNA‐binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells (human 293T and mouse NIH/3T3) than the widely used agent lipofectamine. Therefore, we suggest that this novel goldfish‐derived recombinant histone H1 C‐terminal short peptide could be used as a peptide‐based gene‐transfer mediator.


Biomaterials | 2007

Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide.

Dong Soo Hwang; Sung Bo Sim; Hyung Joon Cha


Biomaterials | 2007

Practical recombinant hybrid mussel bioadhesive fp-151.

Dong Soo Hwang; Youngsoo Gim; Hyo Jin Yoo; Hyung Joon Cha


Bioconjugate Chemistry | 2007

Facile and Rapid Direct Gold Surface Immobilization with Controlled Orientation for Carbohydrates

Jeong Hyun Seo; Kyouichi Adachi; Bong Kuk Lee; Dong Gyun Kang; Yeon Kyu Kim; Kyoung Ro Kim; Hea Yeon Lee; Tomoji Kawai; Hyung Joon Cha


Journal of Biotechnology | 2008

Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture.

Kyoung Ro Kim; Yeon Kyu Kim; Hyung Joon Cha


Enzyme and Microbial Technology | 2007

Universal degenerate oligonucleotide-primed-polymerase chain reaction for detection and amplification of NiFe-hydrogenase genes

Jaoon Y.H. Kim; Hee Jung Jung; Hyung Joon Cha


Korean Journal of Chemical Engineering | 2007

Enhancement of secreted production of glucoamylase through fed-batch bioreactor culture of recombinant yeast harboring glucose-controllable SUC2 promoter

Hyung Joon Cha; Kyoung Ro Kim; Byeong Hee Hwang; Dae Hee Ahn; Young Je Yoo


Enzyme and Microbial Technology | 2007

Escherichia coli-based expression of functional novel DNA-binding histone H1 from Carassius auratus

Quande Wei; Hee Jung Jung; Dong Soo Hwang; Byeong Hee Hwang; Youngsoo Gim; Hyung Joon Cha


Biotechnology and Bioengineering | 2007

High and compact formation of baculoviral polyhedrin‐induced inclusion body by co‐expression of baculoviral FP25 in Escherichia coli

Lin Li; Young-Soo Kim; Dong Soo Hwang; Jeong Hyun Seo; Hee Jeong Jung; Juan Du; Hyung Joon Cha


Applied Biochemistry and Biotechnology | 2007

Statistical Determination of Optimal Baculovirus Infection Condition for Recombinant Protein Production in Drosophila S2 Cells

Hye Sook Cho; Kyoung Ro Kim; Yeon Kyu Kim; Hyung Joon Cha

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Dong Soo Hwang

Pohang University of Science and Technology

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Kyoung Ro Kim

Pohang University of Science and Technology

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Hee Jung Jung

Pohang University of Science and Technology

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Yeon Kyu Kim

Pohang University of Science and Technology

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Byeong Hee Hwang

Incheon National University

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Hee Jeong Jung

Pohang University of Science and Technology

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Youngsoo Gim

Pohang University of Science and Technology

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Juan Du

Huazhong Agricultural University

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