Hyung Joon Cha
Pohang University of Science and Technology
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Publication
Featured researches published by Hyung Joon Cha.
Biotechnology Progress | 2008
Hee Jung Jung; Dong Soo Hwang; Quan De Wei; Hyung Joon Cha
The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research. Protein‐based gene delivery including histone proteins has recently emerged as a powerful technique for non‐viral DNA transfer. Histones are DNA‐binding proteins that function in DNA packaging and protection. In particular, histone H1 is largely responsible for the stabilization of higher‐order chromatin structures. Several studies have examined the use of full‐length histone H1‐mediated gene transfer, and a few studies have investigated the use of C‐terminal histone H1 fragments as gene‐transfer materials. Previously, we cloned a novel histone H1 cDNA from the goldfish Carassius auratus and found that a recombinant histone H1 C‐terminal short peptide (H1C) of 61 amino acids has comparable DNA binding and protection functions as full‐length histone H1. In the present work, we successfully expressed and purified soluble recombinant H1C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. We confirmed its DNA‐binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells (human 293T and mouse NIH/3T3) than the widely used agent lipofectamine. Therefore, we suggest that this novel goldfish‐derived recombinant histone H1 C‐terminal short peptide could be used as a peptide‐based gene‐transfer mediator.
Biomaterials | 2007
Dong Soo Hwang; Sung Bo Sim; Hyung Joon Cha
Biomaterials | 2007
Dong Soo Hwang; Youngsoo Gim; Hyo Jin Yoo; Hyung Joon Cha
Bioconjugate Chemistry | 2007
Jeong Hyun Seo; Kyouichi Adachi; Bong Kuk Lee; Dong Gyun Kang; Yeon Kyu Kim; Kyoung Ro Kim; Hea Yeon Lee; Tomoji Kawai; Hyung Joon Cha
Journal of Biotechnology | 2008
Kyoung Ro Kim; Yeon Kyu Kim; Hyung Joon Cha
Enzyme and Microbial Technology | 2007
Jaoon Y.H. Kim; Hee Jung Jung; Hyung Joon Cha
Korean Journal of Chemical Engineering | 2007
Hyung Joon Cha; Kyoung Ro Kim; Byeong Hee Hwang; Dae Hee Ahn; Young Je Yoo
Enzyme and Microbial Technology | 2007
Quande Wei; Hee Jung Jung; Dong Soo Hwang; Byeong Hee Hwang; Youngsoo Gim; Hyung Joon Cha
Biotechnology and Bioengineering | 2007
Lin Li; Young-Soo Kim; Dong Soo Hwang; Jeong Hyun Seo; Hee Jeong Jung; Juan Du; Hyung Joon Cha
Applied Biochemistry and Biotechnology | 2007
Hye Sook Cho; Kyoung Ro Kim; Yeon Kyu Kim; Hyung Joon Cha