Hyunkeun Song
Korea Research Institute of Bioscience and Biotechnology
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Publication
Featured researches published by Hyunkeun Song.
Journal of Immunology | 2001
Hyung Sik Kang; Min Ju Lee; Hyunkeun Song; Seung Hyun Han; Yong Man Kim; Joo Young Im; Inpyo Choi
The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH2-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 Ab. Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.
Immunology Letters | 2003
Hyunkeun Song; Daeho Cho; Jun Ho Jeon; Seung Hyun Han; Dae Young Hur; Young Sang Kim; Inpyo Choi
Abstract The expression of vitamin D 3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D 3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.
Biochemical and Biophysical Research Communications | 2002
Daeho Cho; Jae Seung Kang; Jong Hoon Park; Young In Kim; Eunsil Hahm; June-Chul Lee; Yoolhee Yang; Jun-Ho Jeon; Hyunkeun Song; Hyun-Jeong Park; Taesung Kim; Saic Pang; Chul Woo Kim; Young Il Hwang; Wang Jae Lee
Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human keratinocyte cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-L-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.
Immunity | 2005
Kee Nyung Lee; Hyung-Sik Kang; Jun-Ho Jeon; Eun-Mi Kim; Suk-Ran Yoon; Hyunkeun Song; Chil-Youl Lyu; Zheng-Hao Piao; Sun-Uk Kim; Ying-Hao Han; Su-Sung Song; Young-Ho Lee; Kyu-Sang Song; Yong-Man Kim; Dae-Yeul Yu; Inpyo Choi
Cancer Research | 2000
Daeho Cho; Hyunkeun Song; Yong Man Kim; Dong Houh; Dae Young Hur; Hyun-Jeong Park; Do-Young Yoon; Kwang Ho Pyun; Wang Jae Lee; Masashi Kurimoto; Yoon Berm Kim; Young Sang Kim; Inpyo Choi
Immunology Letters | 2005
Jeongsim Hue; Ankeun Kim; Hyunkeun Song; Inpo Choi; Hyun-Jeong Park; Taesung Kim; Wang Jae Lee; Hyung-Sik Kang; Daeho Cho
Journal of Immunology | 2012
Gabin Park; Yeong Seok Kim; Hyun-Kyung Lee; Hyunkeun Song; Seonghan Kim; Daeho Cho; Wang-Jae Lee; Dae Young Hur
Journal of Immunology | 2009
Dae Young Hur; Gabin Park; Yeong-Seok Kim; Hyunkeun Song; Minjung Sung; Hyun-Kyung Lee; Wang Jae Lee
Immunology | 2009
Gabin Park; Hyunkeun Song; Yeong-Seok Kim; Minjung Sung; Jeoung Whan Ryu; Hyun-Kyung Lee; Daeho Cho; Daejin Kim; Wang Jae Lee; Dae Young Hur
The FASEB Journal | 2008
Hyun-Kyung Lee; Yang-Gun Kim; Ga Bin Park; Yeong-Seok Kim; Hyunkeun Song; Wang Jae Lee; Dae Young Hur