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Dive into the research topics where Hyunkeun Song is active.

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Featured researches published by Hyunkeun Song.


Journal of Immunology | 2001

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Hyung Sik Kang; Min Ju Lee; Hyunkeun Song; Seung Hyun Han; Yong Man Kim; Joo Young Im; Inpyo Choi

The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH2-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 Ab. Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.


Immunology Letters | 2003

Vitamin D3 up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species

Hyunkeun Song; Daeho Cho; Jun Ho Jeon; Seung Hyun Han; Dae Young Hur; Young Sang Kim; Inpyo Choi

Abstract The expression of vitamin D 3 up-regulating protein-1 (VDUP1) was up-regulated by 1α,25-dihydroxyvitamin D 3 (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.


Biochemical and Biophysical Research Communications | 2002

The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT)

Daeho Cho; Jae Seung Kang; Jong Hoon Park; Young In Kim; Eunsil Hahm; June-Chul Lee; Yoolhee Yang; Jun-Ho Jeon; Hyunkeun Song; Hyun-Jeong Park; Taesung Kim; Saic Pang; Chul Woo Kim; Young Il Hwang; Wang Jae Lee

Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human keratinocyte cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-L-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.


Immunity | 2005

VDUP1 Is Required for the Development of Natural Killer Cells

Kee Nyung Lee; Hyung-Sik Kang; Jun-Ho Jeon; Eun-Mi Kim; Suk-Ran Yoon; Hyunkeun Song; Chil-Youl Lyu; Zheng-Hao Piao; Sun-Uk Kim; Ying-Hao Han; Su-Sung Song; Young-Ho Lee; Kyu-Sang Song; Yong-Man Kim; Dae-Yeul Yu; Inpyo Choi


Cancer Research | 2000

Endogenous Interleukin-18 Modulates Immune Escape of Murine Melanoma Cells by Regulating the Expression of Fas Ligand and Reactive Oxygen Intermediates

Daeho Cho; Hyunkeun Song; Yong Man Kim; Dong Houh; Dae Young Hur; Hyun-Jeong Park; Do-Young Yoon; Kwang Ho Pyun; Wang Jae Lee; Masashi Kurimoto; Yoon Berm Kim; Young Sang Kim; Inpyo Choi


Immunology Letters | 2005

IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity

Jeongsim Hue; Ankeun Kim; Hyunkeun Song; Inpo Choi; Hyun-Jeong Park; Taesung Kim; Wang Jae Lee; Hyung-Sik Kang; Daeho Cho


Journal of Immunology | 2012

ROS-p38 MAPK-Fas/Fas Ligand-Linked Cascade Regulate Bax Translocation and Calcium Redistribution in Salubrinal-Induced Apoptosis of EBV-Transformed B Cells

Gabin Park; Yeong Seok Kim; Hyun-Kyung Lee; Hyunkeun Song; Seonghan Kim; Daeho Cho; Wang-Jae Lee; Dae Young Hur


Journal of Immunology | 2009

CM1 ligation induces the apoptosis of cervical cancer Hela 229 cells via Fas/FasL interaction

Dae Young Hur; Gabin Park; Yeong-Seok Kim; Hyunkeun Song; Minjung Sung; Hyun-Kyung Lee; Wang Jae Lee


Immunology | 2009

Cell cycle arrest induced by engagement of B7-H4 on EBV-positive B cell lymphoma cell lines.

Gabin Park; Hyunkeun Song; Yeong-Seok Kim; Minjung Sung; Jeoung Whan Ryu; Hyun-Kyung Lee; Daeho Cho; Daejin Kim; Wang Jae Lee; Dae Young Hur


The FASEB Journal | 2008

CM1 ligation induces the apoptosis of human lung cancer cells via Fas/FasL interaction

Hyun-Kyung Lee; Yang-Gun Kim; Ga Bin Park; Yeong-Seok Kim; Hyunkeun Song; Wang Jae Lee; Dae Young Hur

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Wang Jae Lee

Seoul National University

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Daeho Cho

Sookmyung Women's University

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Inpyo Choi

Korea Research Institute of Bioscience and Biotechnology

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Hyun-Jeong Park

Catholic University of Korea

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Hyung-Sik Kang

Chonnam National University

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Jun-Ho Jeon

Korea Research Institute of Bioscience and Biotechnology

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