I. Irem Tatli

Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam.

ETHNOPHARMACOLOGICAL RELEVANCE The leaves, flowers and whole aerial parts of Verbascum L. species have been used to treat respiratory problems, haemorrhoids and other types of inflammatory conditions in traditional Turkish medicine. AIM OF THE STUDY In order to evaluate this traditional information, the anti-inflammatory, antinociceptive and wound healing activities of Verbascum mucronatum Lam. which is used as haemostatic in Turkish folk medicine were investigated. MATERIALS AND METHODS In vivo inhibitory effect of the extracts on the carrageenan-induced hind paw edema model in mice was studied for the assessment of anti-inflammatory activity. Moreover, the wound healing potential of the plant were evaluated by using in vivo wound healing experimental models, i.e. incision and excision models on mice and rats, were comparatively assessed with a reference ointment Madecassol(®). Skin samples were also evaluated histopathologically. RESULTS The results of these experimental studies exhibited that Verbascum mucronatum displays anti-inflammatory, antinociceptive and wound healing activities. Through bioassay-guided fractionation and isolation procedures four iridoid glucosides, ajugol (1), aucubin (2), lasianthoside I (3), catalpol (4), two saponins, ilwensisaponin A (5) and C (6) and a phenylethanoid glycoside, verbascoside (7) were isolated and their structures were elucidated by spectral techniques. Verbascoside (7) was found to possess significant wound healing activity as well as antinociceptive and anti-inflammatory potentials, per os without inducing any apparent acute toxicity or gastric damage. CONCLUSION The experimental study revealed that Verbascum mucronatum displays remarkable antinociceptive, anti-inflammatory and wound healing activities.

Antinociceptive and Anti-Inflammatory Effects of Saponin and Iridoid Glycosides from Verbascum pterocalycinum var. mutense Hub.-Mor.

The anti-inflammatory and antinociceptive properties of four major compounds from the flowers of Verbascum pterocalycinum var. mutense were investigated. Saponin glycosides called ilwensisaponin A and C and iridoid glycosides known as ajugol and picroside IV were isolated from the methanolic extract. A dose-related anti-inflammatory and antinociceptive response were obtained in this study at doses of 100 and 200 mg/kg. The results of the evaluation of the anti-inflammatory activity induced by carrageenan and PGE1 showed that this species possesses active constituents that could diminish the cyclooxygenase activitiy. No effects were observed in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model. Our results support the anti-inflammatory and analgesic effects of Verbascum pterocalycinum var. mutense. Ilwensisaponins A and C could explain in part the anti-inflammatory and analgesic activities of this species. Although antinociceptive and anti-inflammatory activities of ajugol and picroside IV were found insignificant in the statistical analysis, ilwensisaponin A and C showed notable activity without inducing any apparent acute toxicity as well as gastric damage.

The effects of Feijoa sellowiana fruits on the antioxidant defense system, lipid peroxidation, and tissue morphology in rats

Context: The fruits of Feijoa sellowiana Berg. (Myrtaceae) have been used to treat goiter in traditional Turkish medicine. Objective: To evaluate the in vivo antioxidant activities of different polarities of the fruit extracts in blood and tissue (liver, kidney, brain, and heart) antioxidant defense systems in standard pellet diet and in high fat diet consumed, male rats were assessed. Materials and methods: The extracts (methanol, n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous) were administered orally to male rats at 50 mg/kg doses daily for 4 weeks. The blood and tissue malondialdehyde (MDA), reduced glutathione (GSH) levels, plasma nitrate (NOx) level, total triiodothyronine (T3), thyroxine, cholesterol, triglyceride, protein, and glucose levels were determined, and erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities; plasma antioxidant activity (AOA) were experimentally studied. Results: Blood MDA level (7.81 ± 0.4) was significantly decreased; GSH level (29.65 ± 1.21) and AOA (1.52 ± 0.08) were increased in ethyl acetate extract as compared with control and the other extracts. In addition, all the extracts decreased MDA levels and increased GSH levels (except brain tissue homogenate) in the tissue homogenates. Erythrocyte SOD and CAT activity levels were unchanged in F. sellowiana extracts. However, the extracts had no effect on plasma NOx. In the histopathological examinations, any changes or damage in the vital organs were seen in animals. Conclusion: The experimental data demonstrated that F. sellowiana extracts displayed remarkable antioxidant activity and decreased lipid peroxidation in rats; furthermore, no histopathological changes or damage have been observed in the vital organs of rats.

An ethnopharmacological study on Verbascum species: from conventional wound healing use to scientific verification.

AIM OF THE STUDY The leaves, flowers, and whole aerial parts of Verbascum L. (Scrophulariaceae) species are used to treat eczema and other types of inflammatory skin conditions and as a desiccant for wounds in Turkish traditional medicine. In the present study, the methanolic extracts of 13 Verbascum species growing in Turkey, including Verbascum chionophyllum Hub.-Mor., Verbascum cilicicum Boiss., Verbascum dudleyanum (Hub.-Mor.) Hub.-Mor., Verbascum lasianthum Boiss., Verbascum latisepalum Hub.-Mor., Verbascum mucronatum Lam., Verbascum olympicum Boiss., Verbascum pterocalycinum var. mutense Hub.-Mor., Verbascum pycnostachyum Boiss. & Heldr., Verbascum salviifolium Boiss., Verbascum splendidum Boiss., Verbascum stachydifolium Boiss. & Heldr and Verbascum uschackense (Murb.) Hub.-Mor. were assessed for their in vivo wound healing activity. MATERIAL AND METHODS In vivo wound healing activity of the plants were evaluated by linear incision and circular excision experimental models subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol(®), which contains 1% extract of Centella asiatica. RESULTS The methanolic extracts of Verbascum olympicum, Verbascum stachydifolium and Verbascum uschackense demonstrated the highest activities on the both wound models. Moreover, the methanolic extracts of Verbascum latisepalum, Verbascum mucronatum, and Verbascum pterocalycinum var. mutense were found generally highly effective. On the other hand, the rest of the species did not show any remarkable wound healing effect. Results of the present study support the continued and expanded utilization of these plant species employed in Turkish folk medicine. CONCLUSION The experimental study revealed that Verbascum species display remarkable wound healing activity.

In vitro anthelmintic effect of Vicia pannonica var. purpurascens on trichostrongylosis in sheep.

Vicia species are used for the treatment of malaria, diarrhea, hemorrhoids, kidney problems and infertility in Turkish traditional medicine. The present study was carried out to evaluate the in vitro anthelmintic effects of Vicia pannonica Crantz. var. purpurascens (DC). Ser. extracts. Larval motility test was used to determine anthelmintic activity of this plant. Motility of the larvae is measured by observation. The methanol, n-hexane, chloroform, acetone, and aqueous extracts of the aerial parts of the plant including the leaves and flowers were applied to developing trichostrongylus larvae at 1, 0.8, 0.6, 0.4, 0.2 and 0.1 mg/ml doses. Thiabendazole and distilled water with 5% DMSO was used as positive and negative control. All of the extracts were 100% effective. Two flavone and flavonol glycosides; luteolin-7-β-O-glucopyranoside (1) and quercetin-3-O-β-glucopyranoside (2) were isolated from the acetone extract and their structures were elucidated by spectral techniques. The solutions prepared from two flavonoid fractions at several doses were performed in vitro to larvae in the same way. Both of them were 100% effective at 1 and 0.8 mg/ml doses. Results of the present study support the utilization of these plant species employed in Turkish folk medicine.

Biotechnological Production of Plant Secondary Metabolites

Description: Modern techniques have been developed to overcome problems associated with the extraction of natural products from plants. These techniques include production of secondary metabolites by biotechnological methods such as plant tissue culture and microbial biotransformation of natural substances. Such methods have led to an increased yield of secondary metabolite amount, the discovery of new biochemical derivatives and agricultural development. For instance, use of these techniques in agricultural area have led to some beneficial traits such as formation of new varieties of known plant species, better crop quality, higher yield, better nutritive properties, more resistant species to insects and pests. Microbial biotransformation is beneficial in plant secondary metabolite production and derivatization (chemical modification of any compound made by a microorganism) and is chiefly applicable to several varieties of terpenes and steroids. This E-book demonstrates recent developments in this field. It will be of particular interest to the professionals in pharmaceutical and biotechnological industries, as well as natural product chemists, medicinal chemists, plant biochemists, and molecular biologists.

Cholinesterase inhibitory and antioxidant properties of Verbascum mucronatum Lam. and its secondary metabolites.

1 The aqueous extract of Verbascum mucronatum Lam. along with its fractions and secondary metabolites were assessed for their antioxidant, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) inhibitory activities. The antioxidant activity was evaluated by three methods: as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion-chelating effect, and ferric-reducing antioxidant power (FRAP) tests. The AChE activity was determined by the Ellman method using an ELISA microplate reader. Phytochemical investigations revealed the presence of four iridoid glucosides, ajugol (), aucubin (2), lasianthoside I (3) and catalpol (4), two saponins, ilwensisaponin A (5) and C (6), and a phenylethanoid glycoside, verbascoside (7), in Verbascum mucronatum. Their structures were elucidated by spectral techniques. The aqueous extract and fractions including the phenylethanoid glycoside 7 showed DPPH scavenger effect and had the best FRAP. Besides these results, one of the phenylethanoid fractions displayed the highest ferrous ion-chelating effect. While only 7 was found to possess moderate AChE inhibition, the extract, fractions, and all other tested compounds did not inhibit AChE and BChE

Secondary metabolites from the aerial parts of Verbascum dudleyanum and their biological activities

From the overground parts of Verbascum dudleyanum, six iridoid glycosides, aucubin, ajugol, catalpol, 6-O-α-L-rhamnopyranosylcatalpol, saccatoside, and 6-O-(3″-O-trans-p-coumaroyl)-α-L-rhamnopyranosylcatalpol, and two saponins, ilwensisaponin A and C, as well as a flavonoid, luteolin-7-O-β-glucopyranoside, together with an acetophenone glucoside, picein, were isolated. The structures of isolated compounds were elucidated by spectroscopic methods. These compounds showed biological acitivites.

Tyrosinase and Cholinesterase Inhibitory Potential and Flavonoid Characterization of Viola odorata L. (Sweet Violet)

Inhibitory potential of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Viola odorata L. (VO) was investigated against tyrosinase (TYR) and cholinesterases by microplate assays. The antioxidant activity was tested using six in vitro assays. Only the ethanol extract inhibited TYR (80.23 ± 0.87% at 100 µg mL−1), whereas none of them were able to inhibit cholinesterases. The extracts were more able to scavenge NO radical (31.98 ± 0.53–56.68 ± 1.10%) than other radicals tested, and displayed low to moderate activity in the rest of the assays. HPLC analysis revealed that the aqueous extract of VO contained a substantial amount of vitexin (18.81 ± 0.047 mg g−1 extract), while the ethanol extract also possessed rutin (1.31 ± 0.013 mg g−1 extract) and vitexin (4.65 ± 0.103 mg g−1 extract). Furthermore, three flavonoids (rutin, isovitexin, and kaempferol‐6‐glucoside) were isolated from the ethanol extract. This is the first report on TYR inhibitory activity of VO as well as presence of vitexin and isovitexin in this species. Copyright

Antinociceptive and Anti-inflammatory Activities of Seven Endemic Verbascum Species Growing in Turkey

The leaves, flowers, and whole aerial parts of Verbascum L. (Scrophulariaceae) species have been used to treat respiratory problems, eczema, and other types of inflammatory skin conditions in traditional Turkish medicine. Moreover, they have also been widely used as a folk medicine to produce a soothing and anti-inflammatory effect on the urinary tract. In order to evaluate their folkloric use, both antinociceptive and anti-inflammatory activities of seven endemic Verbascum species [V. chionophyllum Hub.-Mor., V. cilicicum Boiss., V. dudleyanum (Hub.-Mor.) Hub.-Mor., V. latisepalum Hub.-Mor., V. pycnostachyum Boiss. & Heldr., V. salviifolium Boiss., V. splendidum Boiss.] were investigated. Antinociceptive activity was investigated via p-benzoquinone–induced writhing test, and the anti-inflammatory activity was studied using carrageenan-induced hind paw edema, PGE2-induced hind paw edema, and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema models in mice. The methanol extracts of the flowers of V. chionophyllum and V. pycnostachyum, and the aerial parts of V. latisepalum and V. salviifolium, displayed significant antinociceptive and anti-inflammatory activity at 200 mg/kg oral dose without inducing any apparent acute toxicity or gastric damage. On the other hand, extracts from the rest of the species did not show any remarkable anti-inflammatory and antinociceptive activity. Results of the current study support the continued and expanded use of these plant species employed in Turkish folk medicine.