I Nyoman Pugeg Aryantha

Growth Promotion of Rice Plant by Endophytic Fungi

The main aim of this research is to obtain endophytic fungi from graminiae which has the ability to promote the growth of rice plant. The research was conducted in laboratory of Mycology Research Center for BioSciences and Biotechnology Institut Teknologi Bandung. Isolation of endophytic fungi was done by direct plating of root samples on the potatoe dextrose agar media after surface sterilization steps. Identification was based on morphology characteristics and DNA marker. At least 124 isolates were obtained from elephant grass (Pennisetum purpureum) (27 isolates), alang-alang (Imperata cylindrica) (25 isolates), sugar cane (Saccharum officinarum) (14 isolates), maize (Zea mays) (9 isolates), rice plant (Oryza sativa) (23 isolates) and cyperus (Cyperus rotundus) (26 isolates). Among them, 12 isolates demosntrated the capability to promote the growth of rice seedlings in term of seed germination, plant height, root length and degree of root colonization. Isolate I-11 identified as Phialemonium dimorphosphorum was the best to promote rice plant growth in glass house trial.

Potential MGPB In Optimizing Paddy Straw Mushroom (Volvariella volvacea WW-08) Growth

To evaluate the activity of purified oligosaccharides (POS) as prebiotic from various different local bananas ( Musa sp.); Tanduk (T), Uli (U), Raja Sereh (RS), and Cavendish (C); and to investigate their capacity in promoting the growth of Lactobacillus sp., in vivo. In vitro investigation: Oligosaccharides from various different local bananas were purified by 80% ethanol extraction. Subsequently, absolute ethanol was reconstituted before precipitation/centrifugation for glucose removal. Water was also removed by freeze drying. POS from the four bananas were analyzed by Thin Layer Chromatography (TLC). Prebiotic activity of POS was investigated by measurement of Prebiotic Activity Score (PAS). In vivo investigation: Balb/c mice were grouped into 6 groups with different prebiotics supplementation: negative control (4 mice, standard feed), positive control (6 mice, 15 mg of inulin/g/day), and samples (5 mice, 150 mg of T, U, RS, or C banana/g/day) for 40 days. Following 40 days after treatment, fecal viable counts of Lactobacillus sp. and Enterobacteriaceae of Balb/c mice was measured (CFU/g) and analysed. PAS value revealed a positive correlation between the oligosaccharides from bananas and Lactobacillus paracasei , with PAS value for T (0.05), RS (0.15), U (0.33) and C (0.77). Overall data suggest that fecal viable counts of Lactobacillus sp. increased after 25 days administration of U, RS, and C banana when compared to controls. Contrastingly, the fecal viable counts of Enterobacteriaceae decreased after 40 days administration of U, RS, and C banana compared to the control. Different types of local bananas demonstrate diverse prebiotic activities: U and C promote Lactobacillus sp. growth and reduce Enterobacteriaceae count. PAS value of U and C suggest potential prebiotic activity, whereas T and RS do not.