I. Watson

Random phase encoding for optical security

A new optical encoding method for security applications is proposed. The encoded image (encrypted into the security products) is merely a random phase image statistically and randomly generated by a random number generator using a computer, which contains no information from the reference pattern (stored for verification) or the frequency plane filter (a phase‐only function for decoding). The phase function in the frequency plane is obtained using a modified phase retrieval algorithm. The proposed method uses two phase‐only functions (images) at both the input and frequency planes of the optical processor leading to maximum optical efficiency. Computer simulation shows that the proposed method is robust for optical security applications.

Heat transfer analysis of Staphylococcus aureus on stainless steel with microwave radiation

Staphylococcus aureus (NCTC 6571; Oxford strain) on stainless steel discs was exposed to microwave radiation at 2450 MHz and up to 800 W. Cell viability was reduced as the exposure time increased, with complete bacterial inactivation at 110 s, attaining a temperature of 61·4 °C. The low rate of temperature rise, RT, of the bacterial suspension as compared with sterile distilled water or nutrient broth suggests a significant influence of the microwave sterilization efficacy on the thermal properties of the micro‐organisms. The heat transfer kinetics of thermal microwave irradiation suggest that the micro‐organism has a power density at least 51‐fold more than its surrounding liquid suspension. When the inoculum on the stainless steel disc was subjected to microwave radiation, heat conduction from the stainless steel to the inoculum was the cause of bacteriostasis with power absorbed at 23·8 W for stainless steel and 0·16 W for the bacteria‐liquid medium. This report shows that the microwave killing pattern of Staph. aureus on stainless steel was mainly due to heat transfer from the stainless steel substrate and very little direct energy was absorbed from the microwaves.

Bactericidal action of high-power Nd:YAG laser light on Escherichia coli in saline suspension

Infra‐red light (1064 nm) from a high‐power Nd:YAG laser caused more than 90% loss of viability of Escherichia coli during exposures that raised the temperature of PBS suspensions of the bacteria to 50 °C in a thermocouple‐equipped cuvette. In contrast, there was minimal loss of viability after heating the same suspensions to 50 °C in a water‐bath, or in a PCR thermal cycler. The mechanism of laser killing at 50 °C was explored by differential scanning calorimetry, by laser treatment of transparent and turbid bacterial suspensions, and by optical absorbancy studies of E. coli suspensions at 1064 nm. Taken together, the data suggested that the bactericidal action of Nd:YAG laser light at 50 °C was due partly to thermal heating and partly to an additional, as yet undefined, mechanism. Scanning electron microscopy revealed localized areas of surface damage on laser‐exposed E. coli cells.

Inactivation of bacteria and yeasts on agar surfaces with high power Nd : YAG laser light

G.D. WARD, I.A. WATSON, D.E.S. STEWART‐TULL, A.C. WARDLAW AND C.R. CHATWIN. 1996. Near infrared light from a high‐powered, 1064 nm, Neodymium : Yttrium Aluminium Garnet (Nd : YAG) laser killed a variety of Gram‐positive and Gramnegative bacteria and two yeasts, lawned on nutrient agar plates. A beam (crosssectional area, 1.65 cm2) of laser light was delivered in 10 J, 8 ms pulses at 10 Hz, in a series of exposure times. For each microbial species, a dose/response curve was obtained of area of inactivation vs energy density (J cm−2). The energy density that gave an inactivation area (IA) equal to 50% of the beam area was designated the IA50‐value and was plotted together with its 95% confidence limits. Average IA50‐values were all within a threefold range and varied from 1768 J cm−2 for Serratia marcescens to 4489 J cm−2 for vegetative cells of Bacillus stearothermophilus. There were no systematic differences in sensitivity attributable to cell shape, size, pigmentation or Gram reaction. At the lowest energy densities where inactivation was achieved for the majority of organisms (around 2000 J cm−2), no effect was observed on the nutrient agar surface, but as the energy density was increased, a depression in the agar surface was formed, followed by localized melting of the agar.

Bactericidal effects of high-power Nd:YAG laser radiation on Staphylococcus aureus

The effect of laser radiation on Staphylococcus aureus 6571 (Oxford strain) was studied with high-power Nd:YAG laser radiation between 50 and 300 W. A range of laser pulse repetition frequencies (PRF) from 5 to 30 Hz, with a combination of pulse energies from 2 to 30 J were applied; this covered a range of energy densities from 800 to . The area of inactivation of S. aureus, lawned on nutrient agar plates, was quantified as a function of energy density and exposure time. The shortest exposure time which produced an area of inactivation equal to 50% of the beam area was achieved at a PRF of 30 Hz, pulse energy of 10 J, and with an exposure time of 10.75 s; this was equivalent to an applied energy density of . No bacterial inactivation was observed at relatively low-power settings for PRF, pulse energies and exposure time of: 20 Hz, 3 J and 34 s; 25 Hz, 2 J and 45 s and 30 Hz, 2 J and 35 s, respectively. These results shows that pulse energy, PRF and exposure time are important criteria when considering inactivation of micro-organisms by laser radiation.

Comparative bactericidal activities of lasers operating at seven different wavelengths

Seven laser instruments, delivering radiation at a selection of wavelengths in the range of 0.355 to 118 mm, were investigated for their ability to kill Escherichia coli as a lawn of the bacteria on nutrient agar culture plates. Easily the most effective was a 600-W CO2 laser operating at 10.6 mm, which produced 1.2- cm2 circular zones of sterilization at energy densities of around 8 J cm22 in a 30-msec exposure. Circular zones with an area of 0.7 cm2 were achieved with 200 W from a Nd:YAG laser delivering 8-ms, 10-J pulses of 1.06 mm radiation at 20 Hz. The exposure time, however, was 16 s and the energy density (1940 J cm22) was more than 240 times higher than with the CO2 laser. This difference is believed to be partly due to the much higher absorption of radiation at 10.6 mm than at 1.06 mm, by water in the bacterial cells and the surrounding medium (nutrient agar). Sterilization was observed after exposure to frequency-tripled Nd:YAG laser radiation at 355 nm (3.5 J cm22). Lasers that were totally ineffective in killing Escherichia coli (with their wavelength and maximum energy densities tested) were the far infrared laser (118 mm; 7.96 J cm22), the laser diode array (0.81 mm; 13,750 J cm22), and the argon ion laser (0.488 mm; 2210 J cm22). The speed at which laser sterilization can be achieved is particularly attractive to the medical and food industries.

High-concentration Er:YAG single-crystal fibers grown by laser-heated pedestal growth technique

High-concentration Er:YAG single-crystal fibers have been grown using the laser-heated pedestal growth technique. Instability in the melt and concomitant opacity of fibers were observed at source concentrations higher than 15 mol.%. Spectroscopic examination shows that broadening of the linewidth of the ⁴I(13/2) → ⁴I(15/2) transition is strongly dependent on Er³⁺ concentration.

Characterization of CO2 and diode laser welding of high carbon steels

Deep penetration welding with a high power CO2 and diode laser may offer an attractive means to join metal for certain applications, for instance: welding car drive shafts and gear plant. The rapid cooling rate of laser welding results in high hardness discontinuities across the welded joint; however, this leads to brittle weld and fatigue failure. To avoid this critical problem, it is useful to optimize the laser operating parameters in order to improve the mechanical properties of the weld. In this study, an experimental analysis was used to predict the significant effect of the weld quality using CO2 and high power diode laser (HPDL) laser welding, operating at 10.6 μm and 810 nm, respectively. Investigations into the weld quality were done to quantify the effect of different welding velocities by examining the hardness profiles, tensile strength, aspect, weld volume formation rate, and microstructure formation. In all cases, the results showed that for the HPDL weld configurations, cracking was observed in the fusion zone, whereas, for a CO2 laser weld, a greater weld strength and wider weld width were observed. For HPDL welding, center-line cracking was found along the fusion zone at higher welding velocities.Deep penetration welding with a high power CO2 and diode laser may offer an attractive means to join metal for certain applications, for instance: welding car drive shafts and gear plant. The rapid cooling rate of laser welding results in high hardness discontinuities across the welded joint; however, this leads to brittle weld and fatigue failure. To avoid this critical problem, it is useful to optimize the laser operating parameters in order to improve the mechanical properties of the weld. In this study, an experimental analysis was used to predict the significant effect of the weld quality using CO2 and high power diode laser (HPDL) laser welding, operating at 10.6 μm and 810 nm, respectively. Investigations into the weld quality were done to quantify the effect of different welding velocities by examining the hardness profiles, tensile strength, aspect, weld volume formation rate, and microstructure formation. In all cases, the results showed that for the HPDL weld configurations, cracking was observ...

Effect of laser and environmental parameters on reducing microbial contamination of stainless steel surfaces with Nd:YAG laser irradiation

Aims:  The effect of laser (pulse repetition frequency, pulse energy and exposure time) and environmental parameters (pH, NaCl concentration and wet or dry samples) of Nd:YAG laser decontamination of stainless steel inoculated with Escherichia coli, Staphylococcus aureus and Listeria monocytogenes was investigated.

Experimental Systems Implementation of a Hybrid Optical-Digital Correlator

A high-speed hybrid optical-digital correlator system was designed, constructed, modeled, and demonstrated experimentally. This correlator is capable of operation at approximately 3000 correlations/s. The input scene is digitized at a resolution of 512 x 512 pixels and the phase information of the two-dimensional fast Fourier transform calculated and displayed in the correlator filter plane at normal video frame rates. High-fidelity reference template images are stored in a phase-conjugating optical memory placed at the nominal input plane of the correlator and reconstructed with a high-speed acousto-optic scanner; this allows for cross correlation of the entire reference data set with the input scene within one frame period. A high-speed CCD camera is used to capture the correlation-plane image, and rapid correlation-plane processing is achieved with a parallel processing architecture.