Ian D. Buchanan

Seasonal variability of the reduction in estrogenic activity at a municipal WWTP.

In vitro monitored estrogenicity of municipal wastewater influent/effluent samples (collected from September to December from a Northern Canadian biological nutrient removal (BNR) treatment plant serving an urban population of 750,000) were combined with operational, wastewater quality, and climate data to determine which of these latter variables may be related to the levels and reduction in the former parameter. Significant variability was present in operational and wastewater quality parameters throughout the sampling period including a 7 degrees C difference in wastewater temperature Most of the wastewater samples collected during this period show a considerable amount of recombinant yeast assay (RYA) activity with the greatest activity (estradiol (E2)-equivalents of 106-175ng/L) seen in the final effluents collected from mid-September to mid-October. Percent reduction in the levels of RYA measured E2-equivalents varied from -234% to 75%. No correlations were seen in RYA activity reduction with percent reduction in 5-day biochemical oxygen demand (BOD(5)), flow (i.e. inversely related to hydraulic retention time), solids retention time or even rainfall, and the reduction trends for RYA measured activity were explained best by ambient and effluent temperatures in an inverse fashion (% reduction in E2-equivalents=-10.8.(effluent temperature in degrees C)+191, p=0.005). Complementary instrumental analysis of select sample composites revealed that the free/conjugated estrogen ratio was indeed greater in the wastewater sampled during warmer temperatures.

Reactor performance and microbial community dynamics during anaerobic co-digestion of municipal wastewater sludge with restaurant grease waste at steady state and overloading stages

Linkage between reactor performance and microbial community dynamics was investigated during mesophilic anaerobic co-digestion of restaurant grease waste (GTW) with municipal wastewater sludge (MWS) using 10L completely mixed reactors and a 20day SRT. Test reactors received a mixture of GTW and MWS while control reactors received only MWS. Addition of GTW to the test reactors enhanced the biogas production and methane yield by up to 65% and 120%, respectively. Pyrosequencing revealed that Methanosaeta and Methanomicrobium were the dominant acetoclastic and hydrogenotrophic methanogen genera, respectively, during stable reactor operation. The number of Methanosarcina and Methanomicrobium sequences increased and that of Methanosaeta declined when the proportion of GTW in the feed was increased to cause an overload condition. Under this overload condition, the pH, alkalinity and methane production decreased and VFA concentrations increased dramatically. Candidatus cloacamonas, affiliated within phylum Spirochaetes, were the dominant bacterial genus at all reactor loadings.

Pilot scale anaerobic co-digestion of municipal wastewater sludge with biodiesel waste glycerin

The effect on process performance of adding increasing proportions of biodiesel waste glycerin (BWG) to municipal wastewater sludge (MWS) was studied using two 1300 L pilot-scale digesters under mesophilic conditions at 20 days SRT. The highest proportion of BWG that did not cause a process upset was determined to be 23% and 35% of the total 1.04 kg VS/(m(3)d) and 2.38 kg COD/(m(3)d) loadings, respectively. At this loading, the biogas and methane production rates in the test digester were 1.65 and 1.83 times greater than of those in the control digester which received only MWS, respectively. The COD and VS removal rates at this loading in the test digester were 1.82 and 1.63-fold those of the control digester, respectively. Process instability was observed when the proportion of BWG in the test digester feed was 31% and 46% of the 1.18 kg VS/(m(3)d) and 2.88 kg COD/(m(3)d) loadings, respectively.

Anaerobic co-digestion of biodiesel waste glycerin with municipal wastewater sludge: microbial community structure dynamics and reactor performance.

Two 10 L completely mixed reactors operating at 37°C and 20 days SRT were used to evaluate the relationships between reactor performance and microbial community dynamics during anaerobic co-digestion of biodiesel waste glycerin (BWG) with municipal wastewater sludge (MWS). The addition of up to 1.35% (v/v) BWG to reactor feeds yielded increased VS and COD removal together with enhanced the biogas production and methane yield. This represented 50% of the MWS feed COD. Pyrosequencing analysis showed Methanosaeta (acetoclastic) and Methanomicrobium (hydrogenotrophic) to be the methanogenic genera present in greatest diversity during stable reactor operation. Methanosaeta sequences predominated at the lowest BWG loading while those of Methanomicrobium were present in greatest abundance at the higher BWG loadings. Genus Candidatus cloacamonas was present in the greatest number of bacterial sequences at all loadings. Alkalinity, pH, biogas production and methane yield declined and VFA concentrations (especially propionate) increased during the highest BWG loading.

The role of mixing in potassium ferrate(VI) consumption kinetics and disinfection of bypass wastewater

Bypass wastewaters need an appropriate auxiliary treatment to address their broad range of chemical and bacterial characteristics. The dual capacity of potassium ferrate(VI) as disinfectant/oxidant and coagulant may be useful in a sustainable process retrofit to provide adequate treatment to such wastewaters. However, the engineering aspects of potassium ferrate(VI) based technology to retrofit within existing coagulation-flocculation-sedimentation basins have not been studied. This study investigated, for the first time, the role of rapid mixing on the rate of potassium ferrate(VI) decay and disinfection in bypass wastewaters from extreme wet weather flow events. First-order, second-order, and double exponential models were fit to the potassium ferrate(VI) consumption data, and the double exponential model was able to represent the potassium ferrate(VI) decay in all conditions with a high coefficient of determination and low mean square error. In addition, Chick-Watson and Hom models were tested in this study, and both fit the E. coli disinfection results. The rates of potassium ferrate(VI) consumption and disinfection derived from the models were higher using 500-1000 rpm rapid mixing speeds than they were when magnetic stirrer mixing was used for the same initial dosage and wastewater sample. There was no significant increase in the potassium ferrate(VI) consumption or disinfection rates with the increase of the rapid mixing speeds from 500 to 1000 rpm which revealed that the reactions were kinetically controlled. The coagulation capability of potassium ferrate(VI) enhanced the sedimentation ability and contributed almost the same as the chemical disinfection capability to the overall E. coli removal. This study suggests that potassium ferrate(VI) can be implemented in existing facilities that mix coagulants to enhance primary sedimentation, yet potassium ferrate(VI) can provide both disinfection and coagulation at lower mixing speeds.