Ian P. Herring

Effect of body position on intraocular pressure in dogs without glaucoma

OBJECTIVE To determine the effects of body position on intraocular pressure (IOP) in dogs without glaucoma. ANIMALS 24 healthy dogs with no evidence of glaucoma. PROCEDURES Dogs underwent ophthalmic examinations to ensure that no IOP-affecting ocular diseases were present. Each dog was sequentially placed in dorsal recumbency, sternal recumbency, and sitting position. For each of the 3 positions, IOP in the right eye was measured by use of an applanation tonometer immediately after positioning (0 minutes) and after 3 and 5 minutes had elapsed. The initial body position was randomly assigned; each position followed the other positions an equal number of times, and IOP measurements were initiated immediately after moving from one body position to the next. Proparacaine hydrochloride (0.5%) was applied to the right eye immediately prior to IOP measurements. RESULTS Intraocular pressure was affected by body position. During the 5-minute examination, IOP decreased significantly in dogs that were dorsally recumbent or sitting but did not change significantly in dogs that were sternally recumbent. For the 3 positions, overall mean IOP differed significantly at each time point (0, 3, and 5 minutes). Mean IOP in dorsal recumbency was significantly higher than that in sternal recumbency at 0 and at 3 minutes; although the former was also higher than that in sitting position at 3 minutes, that difference was not significant. CONCLUSIONS AND CLINICAL RELEVANCE Body position affects IOP in dogs. When IOP is measured in dogs, body position should be recorded and consistent among repeat evaluations.

Expression of vascular endothelial growth factor receptor‐1 and ‐2 in normal and diseased canine eyes

OBJECTIVE   To immunohistochemically evaluate expression of vascular endothelial growth factor receptor-1 (VEGFR1) and -2 (VEGFR2) in ocular tissue of healthy dogs and dogs affected with primary glaucoma, uveitic glaucoma, and intraocular neoplasia. SAMPLE POPULATION   Enucleated globes from five dogs with primary glaucoma, five dogs with uveitic glaucoma, six dogs with intraocular neoplasms and three ophthalmically normal control dogs. PROCEDURE   Ocular tissues were obtained from enucleated globes of clinical cases or immediately following euthanasia for control dogs. Tissue sections were stained immunohistochemically for VEGFR1 and VEGFR2 via standard techniques and vascular tissue was qualitatively evaluated. Vascular endothelial VEGFR1 and VEGFR2 expression patterns are reported for normal and diseased ocular tissues. In addition, VEGFR1 and VEGFR2 expression patterns are reported for all normal ocular tissues. RESULTS   A constitutive expression pattern was detected for VEGFR1 by ocular vascular endothelial cells as well as nonvascular cells in the cornea, uvea, lens, and retina. VEGFR2 demonstrated limited expression in normal ocular tissue, but was widely expressed in vascular endothelium of diseased eyes, particularly in pre-iridal fibrovascular membranes. CONCLUSIONS   The results of this study suggest a role for VEGF receptors in both physiologic and pathologic angiogenesis in canine ocular tissue. Manipulation of this pathway may be a rational consideration for therapeutic intervention in canine ocular disease exhibiting pathologic neovascularization.

A new methodology for investigating airbag-induced skin abrasions

Although airbags have been shown to reduce the incidence of life-threatening injuries, they have increased the risk of minor injuries such as those to the skin. Based on the distribution of injuries that can be directly attributed to the airbag itself, it is believed that shear loading exists as a mechanism for these skin injuries. The purpose of this study was to develop a new methodology designed to assess the injury potential from different types of airbag with respect to shear loading. This new methodology utilized a high-speed impactor to accelerate the airbag fabric past a sample of skin. Contact normal forces were monitored by the use of pressure sensors, and fabric velocity was determined from a high-speed video. The abraded skin samples were analysed using light microscopic analysis and ultraviolet light source photography. A new abrasion rating method was developed called the total abrasion score, which allows for quantifiable differentiation between the abrasions caused by different airbag fabric and seam types.

Evaluation of nasolacrimal fluorescein transit time in ophthalmically normal dogs and nonbrachycephalic cats

OBJECTIVE To evaluate fluorescein nasolacrimal transit (NLT) times in ophthalmically normal dogs and nonbrachycephalic cats by use of 2 methods of the Jones test. ANIMALS 73 dogs and 36 cats. PROCEDURES Fluorescein dye was applied to the ocular surface of both eyes by means of a wetted fluorescein strip and, in a subsequent test, by administration of a drop of 0.2% fluorescein solution. During each test, the nares were monitored for the appearance of fluorescein for up to 30 minutes after application. Time of fluorescein appearance at the nares was recorded as NLT time. Recorded variables for all study animals included age, reproductive status, body weight, and Schirmer tear test values. For dogs, skull index, snout length, and cephalic conformation were also recorded. Data were grouped for statistical comparisons according to test results. RESULTS In both dogs and cats, NLT was faster when the fluorescein solution versus fluorescein strip was used. In cats, none of the recorded variables had a significant effect on NLT, irrespective of the testing method used. In dogs, several variables had a significant effect on NLT, including cephalic conformation, snout length, age, and reproductive status, but these findings varied with testing method and testing group. CONCLUSIONS AND CLINICAL RELEVANCE NLT was highly variable in dogs and cats, regardless of testing method used. Assessment of nasolacrimal patency in brachycephalic dogs by use of either method evaluated here is not likely to be clinically useful. In cats, assessment of nasolacrimal patency with the fluorescein drop method was faster and more conclusive than with the fluorescein strip method.

Experimental Analysis of Airbag Seam Design: High-Rate Shear Testing for Skin Abrasions

Approximately 66 per cent of all airbag deployments in the USA result in at least one skin injury, with 47 per cent of these skin injuries attributed directly to the airbag deployment. The purpose of the present study was to evaluate the risk of skin abrasions from the airbag fabric seam design by using a new shear testing methodology. High-rate shear loading was performed with a pneumatic impactor that propelled a section of airbag fabric across porcine skin at 85 m/s. Twenty-seven tests (three control and 24 with fabric) were performed using eight different seam designs. A 40 cm × 10 cm section of airbag fabric with each seam was forced across a 5 cm × 5 cm section of fresh porcine skin that was acquired within 2 h post-mortem. No abrasions were observed in the three control tests, but abrasions were observed in all 24 of the tests conducted using airbag fabric. The unturned, sewn seam orientation resulted in significantly more severe abrasions than the woven, unturned seam orientation (P = 0.01). This new system and results illustrate that different seam designs can result in different skin abrasion risk. Moreover, the data show that severe abrasions can be caused by normal pressures well below the 1.75 MPa injury threshold previously published.

Duration of corneal anesthesia following topical administration of 0.5% proparacaine hydrochloride solution in clinically normal cats

OBJECTIVE To determine duration of corneal anesthesia following topical administration of 0.5% proparacaine hydrochloride solution in domestic shorthair (DSH) cats. ANIMALS 20 clinically normal DSH cats. PROCEDURES Baseline corneal touch threshold (CCT) was established by use of a Cochet-Bonnet aesthesiometer. Treatment consisted of a single 50-microL topical application of an ophthalmic preparation of 0.5% proparacaine solution to a randomly selected eye of each cat. The corneal touch threshold was assessed 1 and 5 minutes after application to the cornea and at 5- minute intervals thereafter for 60 minutes. RESULTS Corneal sensitivity, as determined by Cochet-Bonnet aesthesiometry, was significantly reduced from baseline for 25 minutes following topical administration of ophthalmic proparacaine. Maximal anesthetic effect lasted 5 minutes. CONCLUSIONS AND CLINICAL RELEVANCE As determined by Cochet-Bonnet aesthesiometry, duration of anesthetic effects on the cornea induced by a single topical application of an ophthalmic preparation of 0.5% proparacaine solution in DSH cats is considerably shorter than the reported duration of corneal anesthesia in dogs.

Eye Injury and Orbital Fracture Patterns in Frontal Automobile Crashes

This paper presents the most comprehensive eye injury study to date investigating 10,770,828 weighted occupants from 22,236 cases for the years 1993 through 1999. The purpose of the study was to investigate dye injuries and orbital fractures resulting from frontal automobile crashes and to determine the effects of frontal airbags. The data obtained in this study suggested that airbags increase the overall risk of sustaining any type of eye injury but airbags due decrease the risk of sustaining more serious orbital fractures.

Optic nerve astrocytoma in a dog

Intracranial astrocytomas are relatively uncommon in dogs and optic nerve astrocytomas even more so. This neoplasm should be considered as differential in canine patients with vision loss, retinal detachment, ocular mass, and histopathologic findings of infiltrative fusiform to polygonal glial cells possibly associated with glomeruloid vascular proliferation.