Ichiji Sumitomo

Somatotopic organization in the rat thalamic reticular nucleus.

Mapping experiments were carried out to establish the somatotopic organization of the somatosensory part of the thalamic reticular nucleus (TR) of the rat. Different parts of the body were found to project somatotopically onto the S-TR. The rostral-to-caudal and the dorsal-to-ventral axes in the body parts were transformed into the ventral-to-dorsal and the caudal-to-rostral axes in the S-TR, respectively. The head and face occupied about two thirds of the S-TR, distributing in the ventral half and in the dorsocaudal part. Particularly a large area of the S-TR was devoted to the vibrissae, nose (rhinarium) and lip. The trunk was projected to a small area of the dorsal part. The projections of the hind- and forelimb were mainly in the dorsal part, the former being placed above the latter.

Conduction velocity of optic nerve fibers innervating lateral geniculate body and superior colliculus in the rat

Abstract Conduction velocities of the optic nerve (ON) in the rat were measured by recording evoked unitary discharges of postsynaptic cells to stimulation of the ON at two separate sites. The velocity of ON impulses to activate a given postsynaptic cell was calculated from the distance between the two stimulating sites and the difference in response latency of the same postsynaptic cell. Recordings were made from the lateral geniculate body (LGB) and the superior colliculus (SC), both contralateral to stimulation. ON fibers innervating principal cells of the LGB (P cells of Burke and Sefton) fell into three groups (tg0, tg1 and tg2) having average velocities of 19.2, 11.9, and 5.2 m/sec, respectively. Internuncial cells (I cells) received tg2 fibers only. There were also three groups of ON fibers destined for the SC (tc0, tc1 and tc2). Their average velocities were 15.4, 9.1, and 3.7 m/sec, respectively.

Properties of cells responding to visual stimuli in the rat ventral lateral geniculate nucleus

Abstract Single-unit recordings were made of the ventral lateral geniculate nucleus (LGv) in the albino rat anesthetized with urethane. Visual receptive field properties as well as the characteristics of responses elicited by electrical stimuli to the optic tract and to the visual cortex were examined. Compared with the relay cells of the dorsal lateral geniculate nucleus (LGd), LGv cells were characterized by the following properties. (i) They responded to visual cortex stimuli orthodromically as well as to optic tract shocks. (ii) The postexcitatory inhibition they showed after single optic tract or visual cortex stimuli was only short-lasting, at most 100 ms. (iii) Conduction velocities of the optic nerve afferent fibers were mostly in the range of slow fibers, 2 to 10 m/s. (iv) The receptive fields were essentially homogeneous in type; about 90% of the sample of 53 cells were On-tonic. (v) Receptive field sizes were substantially large, from 6.3 to 45.6° (mean, 22.3°). (vi) On-tonic cells revealed a regular maintained discharge whose level changed monotonically as a function of the luminous intensity of the stimulating light. The functional implications of these findings were compared with those of the relay cells in the LGd.

Two types of thalamic reticular cells in relation to the two visual thalamocortical systems in the rat.

We found in urethane-anesthetized rats that thalamic reticular (TR) cells responding to an electrical stimulus of the optic tract (OT) can be further subdivided into two types, viz. S- and L-type cells. S-type cells, which were selectively excited from area 17 of the visual cortex, were characterized by short latency responses (2.3-6.1 ms) to OT stimulation. TR cells activated antidromically from the dorsal lateral geniculate nucleus were all classified as S-type. Long OT latencies (5.2-15.3 ms) and selective excitation from area 18a were peculiar to L-type cells, which showed antidromic responses to the lateral posterior nucleus stimulation. Mapping studies documented that cells belonging to each type were segregated in the thalamic reticular nucleus; L-type cells were located in the most posterior part. It is suggested that S- and L-type cells are inhibitory interneurons modulating activity of geniculocortical and extrageniculocortical projection cells, respectively.

Neuronal organization of rat thalamus for processing information of vibrissal movements.

Vibrissa-responding neurons were searched for in the somatosensory part of the thalamic reticular nucleus (S-TR) and in the ventrobasal nucleus (VB) in urethane-anesthetized rats. More than 90% of the recorded neurons of both species had receptive fields (RFs) on single vibrissae. Movements of RF-vibrissae produced a burst of multiple discharges in S-TR neurons and single spike discharges followed by a prominent suppression of spontaneous discharges in VB neurons. Antidromic invasion from stimulation of the somatosensory cortex in VB neurons was suppressed after RF-vibrissae were stimulated. A possible functional organization comprising VB and S-TR neurons for processing impulses of vibrissal movements was suggested.

Burst discharges associated with phasic hyperpolarizing oscillations of rat ventrobasal relay neurons

Intracellular recordings were made from ventrobasal relay neurons in urethane-anesthetized rats. A series of phasic hyperpolarizations repeated with the spindle rhythm appeared in response to single shocks to the medial lemniscus or spontaneously. On the recovery slope of some phasic hyperpolarizations slow depolarizations (SDs) lasting for 30-50 ms with burst discharges were generated as rebound excitation. The voltage dependency of SDs was proved by changing the membrane potential by current injection. The number of spikes triggered by the SD increased as the SD became larger in amplitude and faster in rising speed.

Burst discharges of thalamic reticular neurons: an intracellular analysis in anesthetized rats

In order to analyze the mechanism of burst discharges intracellular recordings were made from 27 somatosensory thalamic reticular (S-TR) neurons in urethane-anesthetized rats. Burst discharges, composed of 2-7 spikes, were always superposed on a slow depolarization (SD) lasting for 40-60 ms, which appeared only when the membrane was hyperpolarized. The number of spikes superposed on an SD varied depending upon the amplitude of the SD. A single shock stimulation of the lemniscus medialis elicited a series of SDs, each without being preceded by a phasic hyperpolarizing potential. The SDs were repeated with spindle rhythms. Evidence has been provided that EPSPs contribute to the mechanism for triggering SDs. In spontaneous rhythmic SDs occurring with the rhythm of EEG spindles, steps representing EPSPs were recordable on the rising phase of each SD. It is suggested that excitatory synaptic inputs to S-TR neurons with the spindle rhythm are responsible for the rhythmic generation of SDs. Ventrobasal relay neurons are presumed as the source of the inputs.

Retinal inputs to the geniculate relay cells in the eastern chipmunk (Tamias sibiricus asiaticus): a comparison between color and non-color sensitive cells

Single unit recordings were made from the relay cells of the lateral geniculate nucleus in the eastern chipmunk. Of 362 relay cells, 47 cells (13%) were classified as color sensitive and the rest as non-color sensitive cells. Non-color sensitive cells were further classified into 5 subclasses: off-phasic, on-phasic, on-off-phasic, on-tonic and uncommon types. Within the color sensitive cells there were 3 subclasses; blue excited and green inhibited (+B-G), blue inhibited and green excited (-B+G), and blue excited (+B) cells. Retinal afferents to color sensitive relay cells had the following characteristics: ganglion cells of their origin were distributed in the central high density areas of the retina and axonal conduction velocities were in the intermediate range, though they were somewhat slow in +B cells.

Maintained activity and responsiveness to flicker stimulation in rat lateral geniculate neurons

Abstract Using anesthetized rats, tonic maintained discharges in light and dark and flicker responses of individual cells of the lateral geniculate body were studied as functions of the response latency to single shock stimulation of the optic tract. The cells were divided into two types according to whether the maintained discharge in light was stronger (on-cell) or weaker (off-cell) than that in dark. The two types of the cells were encountered almost equally frequently. The frequency of maintained discharge in light of the on-cell decreased as the response latency to optic tract stimulation increased. This same relation held good in the off-cells with regard to their maintained activities in dark. The critical corresponding frequency (CCF), defined as the highest flicker frequency at which the cell responded to individual flashes faithfully, decreased as the response latency increased. This was true with the on-cells as well as the off-cells. The frequency of maintained discharges, determined in light for the on-cells and in dark for the off-cells, had a linear positive correlation to the CCF.