Ichiro Yahara

DEVELOPMENT OF NEW TECHNIQUES FOR IDENTIFICATION, PURIFICATION AND CHARACTERIZATION OF CANCER CELL-SPECIFIC PROTEINS

Publisher Summary This chapter discusses the development of new techniques for identification, purification, and characterization of cancer cell-specific proteins and proteins deficient in cancer cells. The chapter discusses the development of new techniques to identify the proteins based upon multiple criteria. The chapter discusses the development of new techniques to extract and purify these proteins from various cells and tissues, and to characterize these proteins on the basis of their intracellular localization, biological activity, and molecular interaction with other cellular constituents. The chapter describes the roles of cancer cell-specific proteins in altered growth properties associated with cancer cells. It also describes the roles of growth-regulating proteins, which normally function in normal cells but aberrant in cancer cells. The chapter presents new methods to purify HSP90 and the acidic isoform of mouse β-tubulin. The isolated proteins are examined for their properties and intracellular distribution.