Ido J. Bontekoe

Riboflavin and UV light treatment of platelets: a protective effect of platelet additive solution?

Pathogen reduction technologies (PRTs) increase the safety of the blood supply, but are also associated with cell damage. Our aim was to investigate the effect of Mirasol PRT on platelet (PLT) concentrates stored in plasma and whether the use of a PLT additive solution (PAS) is able to improve in vitro quality.

Evaluation of the role of the GPIb‐IX‐V receptor complex in development of the platelet storage lesion

In mice, loss of sialic acid resulting in shedding of glycoprotein (GP) Ibα and GPV has been linked to platelet survival. The aim of this study was to determine whether loss of sialic acid and the GPIb‐IX‐V complex contributes to development of the platelet storage lesion (PSL) in human platelet concentrates (PCs).

Determination of thromboelastographic responsiveness in stored single-donor platelet concentrates

Thromboelastography (TEG) is widely used in hospitals but less commonly in blood banks for evaluation of platelet (PLT) concentrates (PCs). A TEG‐PC assay for testing fresh or stored PLTs must reflect the quality of the PLTs. The added value could be measurement of donor‐dependent PLT quality.

Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processor.

The Reveos system (Terumo BCT) is a fully automated device able to process four whole blood (WB) units simultaneously into a plasma unit, a red blood cell (RBC) unit, and an interim platelet (PLT) unit (IPU). Multiple IPUs can be pooled to form a transfusable PLT product. The aim of our study was to evaluate the quality of components made with the Reveos system from either fresh (2‐8 hr) or overnight‐held WB.

Separation of centrifuged whole blood and pooled buffy coats using the new CompoMat G5: 3 years experience

Semi‐automatic separation devices can be used for the separation of centrifuged whole blood into leucoreduced red cell concentrate (LR‐RCC), plasma and buffy coat (BC) and to make platelet concentrates (PC) from pooled BCs. To improve and to obtain a more uniform and standardized process, the CompoMat G5 (Fresenius) was implemented, a new‐generation semi‐automated device.

Volume‐reduced platelet concentrates: optimization of production and storage conditions

BACKGROUND: Plasma can be removed from platelet (PLT) concentrates (PCs) when volume reduction for PLT transfusion is indicated. Volume‐reduced PCs are currently produced from pooled buffy coat (BC) PCs or apheresis PCs by pretransfusion volume reduction, followed by transfer to a syringe for immediate transfusion. We evaluated the maximal storage time of the volume‐reduced PCs in gas‐permeable containers.

Effect of rate and delay of cooling during initial cooling process: in vitro effect on red cells

Background  Whole blood is stored at room temperature (RT) until processing into components. After separation and filtration, the RCC has to be cooled from RT to +2 – 6°C. Different start times of the cooling process and different cooling rates can be encountered in daily routine. The effect of these parameters of the initial cooling of leucoreduced red cell concentrates (LR‐RCC) on in vitro quality is not known.

Comparison of haemostatic function of PAS-C-platelets vs. plasma-platelets in reconstituted whole blood using impedance aggregometry and thromboelastography.

There are concerns about the haemostatic function of platelets stored in platelet additive solution (PAS). Aim of this study was to compare the haemostatic function of PAS‐C–platelets to plasma–platelets in reconstituted whole blood.

Platelet storage performance is consistent by donor: a pilot study comparing “good” and “poor” storing platelets

In retrospective studies, it has been shown that differences in storage variables of platelet (PLT) concentrates (PCs) are partially donor dependent. It was our aim to prospectively determine the donor effect on PLT quality.

Effect of solvent/detergent-treated pooled plasma on fibrinolysis in reconstituted whole blood

Hyperfibrinolysis has been observed in patients heavily transfused with solvent/detergent‐treated pooled plasma (S/D plasma). We compared coagulation and fibrinolytic variables in blood containing S/D plasma with blood containing fresh‐frozen plasma (FFP), with and without α2‐antiplasmin or tranexamic acid (TXA) supplementation.