Ihj Koh

0096. Evaluation by videomicroscopy (SDF) of the renal cortex microcirculation and convoluted tubules in acute renal failure during severe sepsis. Experimental study

The microcirculatory dysfunction as the triggering event of organ dysfunction in sepsis is a universal concept1-3,but the microcirculatory dysfunction and its relationship with the deterioration of adjacent tissue is still unsolved, thus, the macrocirculation parameters guide therapeutic decisions although the impairment of microcirculation precedes the macrocirculation dysfunction.

0732. Sequential changes in the pattern of liver architecture in the acute phase of severe sepsis under the vision of videomicroscopy. Experimental study

Therapeutic grounded in the specific state of microcirculatory dysfunction in septic patients is still an impediment due to technological limitations, and consequently there is little knowledge about the pathophysiological microcirculatory dynamics associated with organ failure.

Kinetic study of gut and systemic tissue perfusion following one challenge of bacterial translocation

The pathogenesis of sepsis and multiple organ failure has been associated with bacterial translocation (BT). In a previous study we observed intestinal and systemic tissue hypoperfusion 2 hours after a BT process. In this study we examined the perfusion kinetics a longer period after one unique challenge of BT.

Sepsis provokes host's microbiota overgrowth of commensal Gram-negative bacteria and subsequent induction of bacterial translocation in rats

The literature has shown the participation of intestinal microbiota in the genesis of primary infections as well as of sepsis. In this study we examine the role of sepsis on the microbiota by examining the most frequently recovered Gram-negative bacteria (G-).

Influence of bacterial translocation in the genesis of the microcirculation: hypoperfusion in sepsis.

Increasing evidence suggests that bacterial translocation (BT) has been implicated in the pathogenesis of sepsis and multiple organ failure. In this study we examined the role of the mesenteric lymph during the BT process on the intestinal and systemic tissue perfusion in association with nonlethal sepsis.

Comparison between intravital microscopy and laser Doppler microcirculation monitoring methods in experimental sepsis: preliminary results

Sepsis (S) produces regional perfusion abnormalities by causing vasodilatation and blood flow redistribution, and this process mostly affects the mesenteric circulation. In addition, gut mucosal hypoperfusion can perpetuate the inflammatory process and contributes to the multiple organ failure. Thus, following experimental sepsis induction we examined gut blood flow by intravital microscopy and the gut tissue perfusion by laser Doppler, in order to detect the onset of the intestinal microcirculation changes at the acute phase of sepsis.

Can bacterial translocation be a beneficial event

Infection is a major concern in intestinal transplant recipients. Bacterial migration to extraintestinal sites is a central component of the gut hypothesis of sepsis. However, some studies have cited the beneficial effects of bacterial translocation (BT) on the host acquired immune system. We evaluated the role of previous BT on a subsequent BT challenge, examined the BT index in organs as well as changes in white blood cell (WBC) count in mesenteric lymph and blood for correlation with outcomes. Wistar rats (n = 60) were divided into a BT group (n = 20), which underwent inoculation of 10 mL of 10(10) CFU/mL Escherichia coli R-6 confined to the small intestine as opposed to a BT1-14 group (n = 20), which underwent the BT procedure on days 1 and 14 or a S1-BT14 group (n = 20) that received 10 mL of saline on day 1 and the BT procedure on day 14. Half of the animals were killed 2 hours following the BT procedure. Samples from different compartments were collected for culture. Mesenteric lymph and peripheral blood were examined for WBC counts. The other half of the hosts was subjected to outcome evaluation concerning weight gain and mortality. Animals undergoing double BT showed a significantly lower index of bacterial recovery (liver, spleen, and blood) compared with those having a single BT (P < .05). The WBC count of mesenteric lymph cells after double BT was similar to naïve animals, but significantly lower than the single BT group (P < .05). The outcome was unchanged among double BT versus other groups. A previous BT challenge was efficient to generate a host-defense mechanism against a second BT episode induced by intestinal overgrowth with the same bacterial strain.

The gut mesenteric lymph during bacterial translocation carries factor(s) related to mortality in sepsis

Experimental and clinical studies have shown that bacterial translocation (BT) has been implicated in the pathogenesis of sepsis and multiple organ dysfunction syndrome (MODS). In this study we examined the role of the intestinal lymph during the BT process on the clinical outcome in a pre-established sepsis state.

Iron-deficiency anemia increases intestinal bacterial translocation in rats

Bacterial translocation has been related as the main causal event in the genesis of the systemic infection. Particularly in pediatric patients, worse prognosis of systemic infection has been related to malnourishment, impaired immune response and other debilitating diseases. In this study we therefore decided to examine the effect of iron-deficiency anemia in bacterial translocation (BT).

Previous bacterial translocation challenge enhances peripheral blood bacterial clearance in the subsequent sepsis

A bacterial translocation (BT) event has been strongly related to the pathogenesis of sepsis as well as to the sepsis progression to the state of multiple organ failure, and increasing scientific findings have pointed out the beneficial role of BT by building a gut immune defense repertoire. In a previous study, we have demonstrated that a previous BT challenge reduces significantly the translocation index at the second BT challenge with the same bacterial strain. Thereby, in this study we sought to evaluate the effect of previous BT challenge on experimentally induced sepsis, examining the bacterial clearance index from the systemic blood circulation, in order to evaluate its influence on the hosts immunological defense response.