Ilda Abreu

The effects of meteorological factors on airborne fungal spore concentration in two areas differing in urbanisation level

Although fungal spores are an ever-present component of the atmosphere throughout the year, their concentration oscillates widely. This work aims to establish correlations between fungal spore concentrations in Porto and Amares and meteorological data. The seasonal distribution of fungal spores was studied continuously (2005–2007) using volumetric spore traps. To determine the effect of meteorological factors (temperature, relative humidity and rainfall) on spore concentration, the Spearman rank correlation test was used. In both locations, the most abundant fungal spores were Cladosporium, Agaricus, Agrocybe, Alternaria and Aspergillus/Penicillium, the highest concentrations being found during summer and autumn. In the present study, with the exception of Coprinus and Pleospora, spore concentrations were higher in the rural area than in the urban location. Among the selected spore types, spring-autumn spores (Coprinus, Didymella, Leptosphaeria and Pleospora) exhibited negative correlations with temperature and positive correlations both with relative humidity and rainfall level. On the contrary, late spring-early summer (Smuts) and summer spores (Alternaria, Cladosporium, Epicoccum, Ganoderma, Stemphylium and Ustilago) exhibited positive correlations with temperature and negative correlations both with relative humidity and rainfall level. Rust, a frequent spore type during summer, had a positive correlation with temperature. Aspergillus/Penicillium, showed no correlation with the meteorological factors analysed. This knowledge can be useful for agriculture, allowing more efficient and reliable application of pesticides, and for human health, by improving the diagnosis and treatment of respiratory allergic disease.

Airborne pollen concentration in the region of Braga, Portugal, and its relationship with meteorological parameters

The variation in airborne pollen concentrationof the Braga region (Portugal) was studied inspringtime, during the flowering of Vitisvinifera. The data set was obtained for twoconsecutive years (1999 and 2000), using aCour-type sampler.During this period, thirty-six taxa wereobserved in a total of 3,200 pollengrains m-3 of air (CPA). The main pollentypes observed were Olea, Poaceaea,nd Castanea, representing 74% of thepollen spectrum.The airborne pollen concentration (CPA) wassignificantly correlated with certainmeteorological parameters. Pollen concentrationwas positively correlated with temperature andwind direction (East and Northeast) andnegatively correlated with rainfall and numberof rainy days.

Pollen allergenic potential nature of some trees species: a multidisciplinary approach using aerobiological, immunochemical and hospital admissions data.

Trees are considered producers of allergenic pollen. The aims of this work were to characterize the aerobiology of the Platanus, Acer, Salix, Quercus, Betula and Populus pollen, linking it with monthly emergency hospital admissions and to identify the different reactivity levels in sensitized patients. This information would be of great importance to evaluate the convenience of changing the inventory of pollen producer trees related to the risk of allergenic reactions. The study was conducted in Porto, Portugal, from 2005 to 2007. Airborne pollen was sampled using a Hirst-type volumetric trap. The antigenic and allergenic properties of Acer negundo, Betula pendula, Platanus occidentalis, Populus hybrida, Quercus robur and Salix babylonica pollen, collected in public gardens or sidewalks, were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunological techniques using polysensitized-patient sera. Monthly hospital admissions of asthma or dyspnea related with respiratory diseases were obtained from the Emergency Room database of Hospital Geral de Santo António. Tree pollen and hospital admissions were positively correlated. Tree pollen peaked in March which coincides with the hospital admissions maximum. The highest binding affinity was observed with A. negundo, S. babylonica and P. occidentalis pollen extracts and the lowest with P. hybrida. Consistently, Acer and Platanus maximum airborne pollen concentrations were observed during March attaining levels considered moderate to high risk for allergenic reactions. Prominent bands with approximately 71, 35, 31, 22, 19, 16, 14, 13 and 11 kDa were revealed. A 52 kDa band was shared by all analyzed sera. High levels of airborne pollen and emergency hospital admissions were related. High binding affinity of specific IgE to pollen extracts of the most abundant tree pollen present in the atmosphere was observed. Patient sera revealed multiple similar allergenic bands shared by the different extracts. This multidisciplinary approach is useful in day-to-day medical practice to help in diagnostic, therapeutic and allergy alerting system adjusting.

In vitro exposure of acer negundo pollen to atmospheric levels of SO2 and NO2: effects on allergenicity and germination

In the last years, a rising trend of pollen allergies in urban areas has been attributed to atmospheric pollution. In this work, we investigated the effects of SO(2) and NO(2) on the protein content, allergenicity, and germination rate of Acer negundo pollen. A novel environmental chamber was assembled to exposure pollen samples with SO(2) or NO(2) at two different levels: just below and two times the atmospheric hour-limit value acceptable for human health protection in Europe. Results showed that protein content was lower in SO(2)-exposed pollen samples and slightly higher in NO(2)-exposed pollen compared to the control sample. No different polypeptide profiles were revealed by SDS-PAGE between exposed and nonexposed pollen, but the immunodetection assays indicated higher IgE recognition by all sera of sensitized patients to Acer negundo pollen extracts in all exposed samples in comparison to the nonexposed samples. A decrease in the germination rate of exposed in contrast to nonexposed pollen was verified, which was more pronounced for NO(2)-exposed samples. Our results indicated that in urban areas, concentrations of SO(2) and NO(2) below the limits established for human protection can indirectly aggravate pollen allergy on predisposed individuals and affect plant reproduction.

Effect of air pollutant NO2 on Betula pendula, Ostrya carpinifolia and Carpinus betulus pollen fertility and human allergenicity

Pollen of Betula pendula, Ostrya carpinifolia and Carpinus betulus was exposed in vitro to two levels of NO2 (about 0.034 and 0.067 ppm) - both below current atmospheric hour-limit value acceptable for human health protection in Europe (0.11 ppm for NO2). Experiments were performed under artificial solar light with temperature and relative humidity continuously monitored. The viability, germination and total soluble proteins of all the pollen samples exposed to NO2 decreased significantly when compared with the non-exposed. The polypeptide profiles of all the pollen samples showed bands between 15 and 70 kDa and the exposure to NO2 did not produce any detectable changes in these profiles. However, the immunodetection assays indicated higher IgE recognition by patient sera sensitized to the pollen extracts from all exposed samples in comparison to the non-exposed samples. The common reactive bands to the three pollen samples correspond to 58 and 17 kDa proteins.

Cladosporium airborne spore incidence in the environmental quality of the Iberian Peninsula

Abstract Cladosporium spores are ubiquitous in the air and are included in the fungal contaminants that define environmental quality. This paper provides an updated review of their concentration in the Iberian Peninsula, based on data from 12 sampling stations collected using a common non-viable volumetric sampling method and the same subsequent data-treatment techniques. The database comprised 84 annual periods, covering the period 1993–2009. Mean annual total spore concentrations for each station, calculated as the sum of daily concentrations, ranged from 54 459 in Cartagena to 933 485 in Sevilla; other sampling stations recorded annual total spores between 93 052 (Porto) and 579 953 (Mérida). Analysis of annual spore-distribution patterns revealed either one or two peaks (spring and fall) depending on the location and prevailing climate of the area where the sampling stations have been placed. For all stations, average temperature was the meteorological parameter displaying the strongest positive correlation with airborne spore concentrations.

Characterization of soils from the Algarve region (Portugal): A multidisciplinary approach for forensic applications

The Algarve is located at a very short distance from North Africa, in Southern Portugal, and as one of the most touristic regions of Portugal, it is accessible by air, land and sea. It is very susceptible to many illegal activities, such as illegal migration, drug trafficking, kidnapping, and murder, among others. Therefore, an Algarve soils database for forensic purposes is being conducted with the conjunction of geological and palynological methodologies on soils characterization, since this is of fundamental importance to assess reliable evidence on forensic investigations. In this study, the properties of soils from several proximate sites from the Algarve were investigated, namely: (i) colour determined by spectrophotometry; (ii) particle size distribution determined by laser granulometry; (iii) low-field magnetic susceptibility by a susceptibility meter; and (iv) pollen content using a light microscope. Finally, a hierarchical cluster analysis was applied to ascertain the capacity of the different soil properties for discrimination between samples. The study reveals the utility of geobotanical techniques for forensic discrimination of soils. Even though some similarities between some of the samples were found, each one presented a combination of colour, particle size distribution, magnetic susceptibility and pollen features that enable the determination of a fingerprint expected to reveal a specific site for future selection of coastal search areas in the Algarve region.

Changes in the IgE-reacting protein profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen in response to ozone treatment

This study aims to investigate the effects of O3 in protein content and immunoglobulin E (IgE)-binding profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen. Pollen was exposed to O3 in an environmental chamber, at half, equal and four times the limit value for the human health protection in Europe. Pollen total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by SDS-PAGE and immunological techniques using patients’ sera. O3 exposure affected total soluble protein content and some protein species within the SDS-PAGE protein profiles. Most of the sera revealed increased IgE reactivity to proteins of A. negundo and Q. robur pollen exposed to the pollutant compared with the non-exposed one, while the opposite was observed in P. x acerifolia pollen. So, the modifications seem to be species dependent, but do not necessarily imply that increase allergenicity would occur in atopic individuals.

Ozone effects on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen

In this study, the effect of three different ozone (O3) levels on soluble protein content of Acer negundo, Quercus robur and Platanus spp. pollen was investigated. Pollen was directly collected from each plant and exposed to O3 values below, equal and four times the limit for human health protection in an environmental chamber system. With this chamber, it was possible to control and reproduce the O3 levels between different experiments. Total protein content was determined colorimetrically with Coomassie protein assay reagent (Pierce) by the Bradford method. Results showed that O3 has significant effects on pollen causing a decrease in soluble proteins that seem to be dependent on species and gas concentration. Also, the existence of reproducibility in the effects of O3, within the same concentration, on the protein content of pollen was verified.

Influence of meteorological parameters on Olea flowering date and airborne pollen concentration in four regions of Portugal

For calculating the total annual Olea pollen concentration, the onset of the main pollen season and the peak pollen concentration dates, using data from 1998 to 2004, predictive models were developed using multiple regression analysis. Four Portuguese regions were studied: Reguengos de Monsaraz, Valença do Douro, Braga and Elvas. The effect of some meteorological parameters such as temperature and precipitation on Olea spatial and temporal airborne pollen distribution was studied. The best correlations were found when only the pre‐peak period was used, with thermal parameters (maximum temperature) showing the highest correlation with airborne pollen distribution. Independent variables, selected by regression analysis for the predictive models, with the greatest influence on the Olea main pollen season features were accumulated number of days with rain and rainfall in the previous autumn, and temperatures (average and minimum) from January through March. The models predict 59 to 99% of the total airborne pollen concentration recorded and the initial and peak concentration dates of the main Olea pollen season.