Ina Scheuerpflug
Max Planck Society
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Featured researches published by Ina Scheuerpflug.
Molecular Microbiology | 1997
Roland Richard Ryll; Thomas Rudel; Ina Scheuerpflug; Roland Barten; Thomas F. Meyer
Type 4 pili produced by the pathogenic Neisseria species constitute primary determinants for the adherence to host tissues. In addition to the major pilin subunit (PilE), neisserial pili contain the variable PilC proteins represented by two variant gene copies in most pathogenic Neisseria isolates. Based upon structural differences in the conserved regions of PilE, two pilus classes can be distinguished in Neisseria meningitidis. For class I pili found in both Neisseria gonorrhoeae and N. meningitidis, PilC proteins have been implicated in pilus assembly, natural transformation competence and adherence to epithelial cells. In this study, we used primers specific for the pilC2 gene of N. gonorrhoeae strain MS11 to amplify, by the polymerase chain reaction, and clone a homologous pilC gene from N. meningitidis strain A1493 which produces class II pili. This gene was sequenced and the deduced amino acid sequence showed 75.4% and 73.8% identity with the gonococcal PilC1 and PilC2, respectively. These values match the identity value of 74.1% calculated for the two N. gonorrhoeae MS11 PilC proteins, indicating a horizontal relationship between the N. gonorrhoeae and N. meningitidispilC genes. We provide evidence that PilC functions in meningococcal class II pilus assembly and adherence. Furthermore, expression of the cloned N. meningitidis pilC gene in a gonococcal pilC1,2 mutant restores pilus assembly, adherence to ME‐180 epithelial cells, and transformation competence to the wild‐type level. Thus, PilC proteins exhibit indistinguishable functions in the context of class I and class II pili.
Nature | 1995
Thomas Rudel; Ina Scheuerpflug; Thomas F. Meyer
Proceedings of the National Academy of Sciences of the United States of America | 1995
Thomas Rudel; Dirk Facius; Roland Barten; Ina Scheuerpflug; Edgar Nonnenmacher; Thomas F. Meyer
Infection and Immunity | 1999
Ina Scheuerpflug; Thomas Rudel; Roland Richard Ryll; Jasmine Pandit; Thomas F. Meyer
Archive | 1996
Thomas F. Meyer; Thomas Rudel; Ina Scheuerpflug; Eckhard Fischer; Jürgen Maier; Sandra Eickernjäger; Thomas Schwan
Archive | 2003
Thomas F. Meyer; Thomas Rudel; Ina Scheuerpflug; Jürgen Maier; Sandra Eickernjäger; Thomas Schwan; Eckhard Fischer
Archive | 2005
Thomas F. Meyer; Thomas Rudel; Ina Scheuerpflug; Eckhard Fischer; Jürgen Maier; Sandra Eickernjäger; Thomas Schwan
Archive | 1995
Thomas F. Meyer; Thomas Rudel; Ina Scheuerpflug; Eckhard Fischer; Juergen Maier; Sandra Eickernjaeger; Thomas Schwan
Archive | 1994
Thomas F. Meyer; Thomas Rudel; Roland Richard Ryll; Ina Scheuerpflug
Archive | 1994
Thomas Franz Ferdinand Meyer; Thomas Rudel; Roland Richard Ryll; Ina Scheuerpflug