Inês C. Silva

Trait-related responses to habitat fragmentation in Amazonian bats

Summary: Understanding how interspecific variation in functional traits influences species’ capacity to persist in fragments and use patches in fragmented landscapes is fundamental for the creation of effective conservation plans. This study uses phylogenetic comparative methods to investigate which functional traits of bat species are correlated with their vulnerability to fragmentation in a tropical landscape with low fragment–matrix contrast. Bats were captured over two years in eight forest fragments, nine control sites in continuous forest, and in the secondary forest matrix at the Biological Dynamics of Forest Fragments Project, Central Amazon, Brazil. We tested the hypothesis that there is a significant relationship between species functional traits, environmental gradients (continuous forest and fragment interiors, edges and matrix) and patterns of species distribution using phylogenetic generalized least squares (PGLS) models, as well as a combination of RLQ and fourth-corner analyses. Mobility, body mass, wing morphology, and trophic level were the most important traits linked to fragmentation sensitivity based on the PGLS analysis, while body mass and trophic level emerged as the best predictors in the fourth-corner analysis. These last two traits were correlated with the loss of continuous forest characteristics, such as high-stature trees and forest cover. Many animalivorous bat species rarely persist in small fragments ( Synthesis and applications. Functional traits of species and environmental variables jointly predict local variation in patterns of bat occupancy and abundance in fragmented tropical landscapes. To minimize local extinctions, we recommend increasing habitat availability and enhancing structural and functional connectivity at the landscape scale through the creation, restoration and maintenance of corridors and stepping stones. These measures should be coupled with improving matrix quality by promoting secondary forest regeneration and persistence to effectively reduce fragment–matrix contrast.

The use of artificial benthic collectors for assessment of spatial patterns of settlement of megalopae of Carcinus maenas (L.) (Brachyura: Portunidae) in the lower Mira Estuary, Portugal

Artificial benthic collectors have been widely used for the assessment of settlement rates of decapod crustaceans. However, to date no consistent works have addressed spatial patterns of settlement in different estuarine habitats, and no specific studies targeted the interaction of artificial surfaces with the surrounding natural substrate. It may be expected that the artificial surface may produce a different thigmotactic response when compared to the natural substrate, which may limit the use of this technique for assessment of natural settlement rates. In this study the settlement rates of megalopae of the estuarine crab Carcinus maenas were addressed, specifically deploying artificial benthic collectors in different habitats both intertidal and subtidal in the lower Mira estuary. A number of experiments were performed concerning stratification and temporal fluctuations of settlement. Further, the interaction of collector surface with the surrounding substrate was investigated, by comparing settlement rates in natural and artificial substrates in different habitats. Results have shown significant differences in settlement between different estuarine habitats, both in spatially replicated experiments and in a high-resolution temporal experiment. However, comparison between settlement rates in artificial and natural substrates has shown that there is a strong interference between collectors and surrounding substrate, limiting interpretation of results concerning settlement rates in artificial substrate alone.

Is there a better chela to use for geometric morphometric differentiation in brachyuran crabs? A case study using Pachygrapsus marmoratus and Carcinus maenas

Landmark-based geometric morphometric methods were used to test which chela is most suitable to use in population differentiation of brachyuran crabs. Two species were analysed: Pachygrapsus marmoratus , a homochelous species, and Carcinus maenas , a heterochelous species. Variance analysis of shape variables and of each chela was calculated to determine which claw presents less variation within populations. The results showed that the right chela of P. marmoratus is more appropriate to use when the goal is to differentiate populations. In C. maenas , the males major chela and females right chela are more suitable to use, probably due to its heterochely. To test these results, specimens from different geographical locations were compared. Pachygrapsus marmoratus populations presented significant differences in manus shape, and C. maenas populations had differences in polex size and shape. Differences in carapace shape were also encountered between populations of both species. This phenotypic variation was not corroborated with genetic data, which were obtained using the mitochondrial DNA gene cytochrome oxidase I as a marker. These opposite results suggest that environmental factors, such as diet and parasitism, can be determining shape differentiation.

A comparison of population differentiation in two shore crab species with contrasting distribution along the Portuguese coast, using two morphological methodologies

Along the Portuguese coast, Pachygrapsus marmoratus has a continuous distribution on rocky shores. In contrast, Carcinus maenas has a discontinuous distribution, inhabiting estuaries. Surveys along a coastal latitudinal gradient were made to assess the effect of the distribution pattern on population differentiation of these two species. Population differentiation was studied using two different morphometric methodologies: linear-based morphometrics and landmark-based morphometrics. The linear-based analysis revealed no significant morphological differentiation among the eleven P. marmoratus populations. Landmark-based analysis showed that the northern and central populations were more similar in shape than the southern populations. Nevertheless, there was still some overlap in shape that could be due to the continuous distribution of P. marmoratus along the coast, promoting population panmixia. In C. maenas, both morphometric techniques revealed the existence of morphological differentiation among populations. This shape differentiation showed a clinal variation, explained by a higher degree of isolation of populations that might be due to limited larval flow between them. Environmental factors may also play an important role in causing variation of shape. Landmark-based morphometrics yielded stronger evidence of morphological differences among forms than a linear approach, suggesting that this method may be more suitable for analysis of body shape.