Ines Latka

Multimodal imaging to study the morphochemistry of basal cell carcinoma

Basal cell carcinoma is the most abundant malignant neoplasm in humans, the pathology of which is characterized by an abnormal proliferation of basal cells. Basal cell carcinoma can show a variety of different morphologies, which are based on different cellular biology. Furthermore, the carcinoma often grows invisibly to the eye imbedded in the surrounding skin. Therefore, in some cases its clinical detection is challenging. Thus, our work aims at establishing an unsupervised tissue classification method based on multimodal imaging and the application of chemometrics to discriminate basal cell carcinoma from non-diseased tissue. A case study applying multimodal imaging to ex-vivo sections of basal cell carcinoma is presented. In doing so, we apply a combination of various linear and non-linear imaging modalities, i.e. fluorescence, Raman and second-harmonic generation microscopy, to study the morphochemistry of basal cell carcinoma. The joint information content obtained by such multimodal approach in studying various aspects of the malignant tissue alterations associated with basal cell carcinoma is discussed.

Inscription and characterization of Bragg gratings in single-crystal sapphire optical fibres for high-temperature sensor applications

We report the fabrication and high-temperature characteristics of Bragg gratings in single-crystal sapphire fibres inscribed by infrared femtosecond laser pulses. For stable read-out of the multimode Bragg reflection signal, the sapphire fibre was illuminated by broadband light from a superluminescent diode applying a long section of a step-index multimode silica fibre. This evenly distributed excitation of many guided modes in the sapphire fibre resulted in the polychromator measurement of stable reflection peak shapes and allowed for multiplexed temperature sensing up to 1745 °C at remote read-out with repeatability better than 1 °C. During the temperature tests an annealing process of the sapphire grating was observed at temperatures above 1400 °C. Possibilities for further improvement of sensors performance are discussed with a view to avoiding instabilities from the lack of a fibre cladding that shields the sapphire waveguide from the environment.

Multicore fiber with integrated fiber Bragg gratings for background-free Raman sensing

In the last years a variety of fiber optic Raman probes emerged, which are only partly suited for in vivo applications. The in vivo capability is often limited by the bulkiness of the probes. The size is associated with the required filtering of the probes, which is necessary due to Raman scattering inside the fibers. We employed in-line fiber Bragg gratings (FBG) as notch filter for the collection path and integrated them in a novel type of Raman probe. Multicore singlemode fibers (MCSMF) were designed and drawn integrating 19 singlemode cores to achieve better collection efficiency. A Raman probe was assembled with one excitation fiber and six MCSMF with inscribed FBGs as collection fibers. The probe was characterized regarding Raman background suppression, collection efficiency, and distance dependence. First Raman measurements on brain tissue are presented.

Nanoparticle Layer Deposition for Plasmonic Tuning of Microstructured Optical Fibers

Plasmonic nanoparticles with spectral properties in the UV-to-near-IR range have a large potential for the development of innovative optical devices. Similarly, microstructured optical fibers (MOFs) represent a promising platform technology for fully integrated, next-generation plasmonic devices; therefore, the combination of MOFs and plasmonic nanoparticles would open the way for novel applications, especially in sensing applications. In this Full Paper, a cost-effective, innovative nanoparticle layer deposition (NLD) technique is demonstrated for the preparation of well-defined plasmonic layers of selected particles inside the channels of MOFs. This dynamic chemical deposition method utilizes a combination of microfluidics and self-assembled monolayer (SAM) techniques, leading to a longitudinal homogeneous particle density as long as several meters. By using particles with predefined plasmonic properties, such as the resonance wavelength, fibers with particle-adequate spectral characteristics can be prepared. The application of such fibers for refractive-index sensing yields a sensitivity of about 78 nm per refractive index unit (RIU). These novel, plasmonically tuned optical fibers with freely selected, application-tailored optical properties present extensive possibilities for applications in localized surface plasmon resonance (LSPR) sensing.

Single-pulse fiber Bragg gratings and specific coatings for use at elevated temperatures

The technique of recording fiber Bragg gratings (FBGs) with single exposure pulses during the fiber drawing process allows production of such gratings in complex array structures, with high mechanical strength of the fiber and in a simple and cost-efficient way. This is of special interest for the growing field of fiber sensor applications with FBGs. A general advantage of fiber sensor systems is their ability to be used also at elevated temperatures compared with conventional electric or electronic sensors. For this purpose, the fiber itself as well as the grating structure and the fiber coating should be stable under such elevated temperature conditions. We have investigated different coating materials and possibilities of making temperature-stable FBGs of types I and II in the range of 100 degrees C-1000 degrees C with good reflection efficiency by single-pulse exposure during the fiber drawing process.

Combined fiber probe for fluorescence lifetime and Raman spectroscopy

AbstractIn this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated. Graphical AbstractAn image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples

Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin

All-optical microspectroscopic and tomographic tools have a great potential for the clinical investigation of human skin and skin diseases. However, automated optical tomography or even microscopy generate immense data sets. Therefore, in order to implement such diagnostic tools into the medical practice in both hospitals and private practice, there is a need for automated data handling and image analysis ideally implementing automized scores to judge the physiological state of a tissue section. In this contribution, the potential of an image processing algorithm for the automated classification of skin into normal or keloid based on second-harmonic generation (SHG) microscopic images is demonstrated. Such SHG data is routinely recorded within a multimodal imaging approach. The classification of the tissue implemented in the algorithm employs the geometrical features of collagen patterns that differ depending on the constitution, i.e., physiological status of the skin.

Functionalization of Microstructured Optical Fibers by Internal Nanoparticle Mono-Layers for Plasmonic Biosensor Applications

For fully integrated next-generation plasmonic devices, microstructured optical fibers (MOFs) represent a promising platform technology. This paper describes the use of a dynamic technique to demonstrate the wet chemical deposition of gold and silver nanoparticles (NPs) within MOFs. The plasmonic structures were realized on the internal capillary walls of a three-hole suspended core fiber. Electron micrographs, taken of the inside of the fiber holes, confirm the even distribution of the NP in the MOF over a length of up to 6 m. Accordingly, this procedure is highly productive and makes the resulting MOF-based sensors potentially (very) cost efficient. In proof-of-principle experiments with liquids of different refractive indices, the dependence of the localized surface plasmon resonance (LSPR) on the surroundings was confirmed. Comparing Raman spectra of MOFs with and without NP layers, each one filled with crystal violet, a significant signal enhancement demonstrates the usability of such functionalized MOFs for surface-enhanced Raman spectroscopy (SERS) experiments.

ORMOCER Coated Fiber-Optic Bragg Grating Sensors at Cryogenic Temperatures

ORMOCER coated Fiber-Bragg-Gratings (FBGs) were investigated at cryogenic temperatures. Below the Bragg wavelength of uncoated FBG is nearly independent on temperature. ORMOCER coated FBG are temperature dependent over the whole temperature range investigated from 10 to 300 K. For 50-300 K, the ORMOCER coating contributes to an additional linear temperature shift of the Bragg wavelength of 2.4 pm/K. Below 40 K the temperature dependence decreases to 1.0 pm/K. ORMOCER coated FBGs can be used as sensor at cryogenic temperatures.

Diagnosis and screening of cancer tissues by fiber-optic probe Raman spectroscopy

Raman spectroscopy is an emerging biophotonic tool that advanced in recent years due to steady improvements in instrumentation for excitation and collection, and the availability of fiber optic probes. This review describes the principles of fiber optic Raman probes and their applications in cancer research of lung, breast, skin, bladder, brain, cervix, oral cavity and gastrointestinal tract.