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Dive into the research topics where Inés Santiuste is active.

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Featured researches published by Inés Santiuste.


Journal of Immunology | 2007

CD4+CD25+ T Cell-Dependent Inhibition of Autoimmunity in Transgenic Mice Overexpressing Human Bcl-2 in T Lymphocytes

J. González; Esther Tamayo; Inés Santiuste; Regina Marquina; Luis Buelta; Miguel A. González-Gay; Shozo Izui; Marcos López-Hoyos; Jesús Merino; Ramón Merino

Regulation of lymphocyte survival is essential for the maintenance of lymphoid homeostasis preventing the development of autoimmune diseases. Recently, we described a systemic lupus erythematosus associated with an IgA nephropathy in autoimmune-prone (NZW × C57BL/6)F1 overexpressing human Bcl-2 (hBcl-2) in B cells (transgenic (Tg) 1). In the present study, we analyze in detail a second line of hBcl-2 Tg mice overexpressing the transgene in all B cells and in a fraction of CD4+ and CD8+ T cells (Tg2). We demonstrate here that the overexpression of hBcl-2 in T cells observed in Tg2 mice is associated with a resistance to the development of lupus disease and collagen type II-induced arthritis in both (NZW × C57BL/6)F1 and (DBA/1 × C57BL/6)F1 Tg2 mice, respectively. The disease-protective effect observed in autoimmune-prone Tg2 mice is accompanied by an increase of peripheral CD4+CD25+ hBcl-2+ regulatory T cells (Tregs), expressing glucocorticoid-induced TNFR, CTLA-4, and FoxP3. Furthermore, the in vivo depletion of CD4+CD25+ Tregs in (DBA/1 × C57BL/6)F1 Tg2 mice promotes the development of a severe collagen type II-induced arthritis. Taken together, our results indicate that the overexpression of hBcl-2 in CD4+ T cells alters the homeostatic mechanisms controlling the number of CD4+CD25+ Tregs resulting in the inhibition of autoimmune diseases.


European Journal of Immunology | 2005

The Escherichia coli heat-labile enterotoxin induces apoptosis of immature lymphocytes in vivo via a glucocorticoid-dependent pathway

Esther Tamayo; Ramón Merino; Jovanna González‐Rojas; Regina Marquina; Inés Santiuste; José A. Amado; Rino Rappuoli; Giuseppe Del Giudice; Jesús Merino

Escherichia coli heat‐labile enterotoxin (LT) exhibits a broad range of immunomodulatory activities, including the induction of lymphocyte‐programmed cell death. However, the nature of the lymphoid populations sensitive to LT‐induced apoptosis and the mechanisms used by this toxin to promote such activity are still unclear. In this study, we demonstrate that LT induces in mice a rapid increase in the levels of circulating corticosterone, resulting in a dramatic induction of cell death of immature CD4+CD8+, B220+IgM– and IgM+IgD– T and B cell progenitors, respectively. Apoptosis of these cell populations is similar to that reported after experimental treatment with corticosteroids, it is inhibited by mifepristone, a glucocorticoid receptor antagonist, and does not occur in adrenalectomized animals. These results clearly indicate that endogenous glucocorticoids are the mediators of the LT‐induced cell death, which involves Bcl‐2‐dependent apoptotic pathways. The LT‐mediated programmed cell death requires systemic exposure and the enzymatic activity of LT, since a mutant devoid of any enzymatic activity have no pro‐apoptotic effect at any dose tested.


Arthritis & Rheumatism | 2013

p27Kip1 inhibits systemic autoimmunity through the control of Treg cell activity and differentiation

Marcos Iglesias; Jorge Postigo; Inés Santiuste; J. González; Luis Buelta; Esther Tamayo; Jesús Merino; Ramón Merino

OBJECTIVE Despite the importance of Treg cells in the maintenance of immunologic tolerance, the mechanisms that control their generation and activity are unknown. Since the cell cycle inhibitor p27(Kip1) (p27) was involved in T cell anergy, we undertook this study to explore its role in both Treg cell processes. METHODS The development of type II collagen-induced arthritis (CIA) and lupus-like abnormalities was compared between transgenic mice overexpressing human Bcl-2 in T cells (BCL2-TgT mice) and nontransgenic mice that were deficient or not deficient in p27. The contribution of Treg cells to disease evolution was also explored. Finally, the in vitro activity of Treg cells and their differentiation from naive CD4+ cells was compared between these strains of mice. RESULTS BCL2-TgT mice were protected against CIA by a Treg cell-dependent mechanism. In association with this protection, the overexpression of Bcl-2 in T cells enhanced the differentiation and activity of Treg cells. Both Bcl-2 effects were independent of its antiapoptotic activity but dependent on its capacity to induce the expression of p27 that augmented the strength of transforming growth factor β (TGFβ) signaling in T cells. Accordingly, down-modulation of p27 expression in BCL2-TgT mice promoted CIA. In addition, p27 deficiency in aged C57BL/6 mice reduced the number and activity of Treg cells and induced the development of mild lupus-like abnormalities. CONCLUSION Our results point to p27 as a critical regulator of Treg cell differentiation and function through the positive modulation of TGFβ signaling strength in T cells.


Journal of Autoimmunity | 2010

B-cell overexpression of Bcl-2 cooperates with p21 deficiency for the induction of autoimmunity and lymphomas

Inés Santiuste; Luis Buelta; Marcos Iglesias; Fernanda Genre; Francisco Mazorra; Shozo Izui; Jesús Merino; Ramón Merino

Genetic abnormalities predisposing to autoimmunity generally act in a cooperative manner affecting one or several mechanisms regulating immunological tolerance. In addition, many of these genetic abnormalities are also involved in the development of lymphoproliferative diseases. In the present study, we have determined the possible cooperation between deficiencies in members of the Cip/Kip family of cell cycle regulators (p21(WAF1/Cip1) or p27(kip1)) and the overexpression of human Bcl-2 in B lymphocytes in the induction of autoimmune and lymphoproliferative diseases in non-autoimmune C57BL/6 (B6) mice. Unlike single mutant mice, B6.p21(-/-) mice transgenic for human Bcl-2 in B cells developed a lethal autoimmune syndrome characterized by the production of autoantibodies, the prominent expansion of memory B and CD4(+) T cells and the development of severe glomerular lesions resembling IgA nephropathy. Furthermore, these mice presented a high incidence of B-cell lymphoproliferative disorders. Such genetic cooperation in the induction of autoimmunity was not observed in B6.p27(-/-) mice transgenic for human Bcl-2 in B cells. Altogether, what we have demonstrated here is the existence of preferential interactions among particular regulators of the G(1)/S transition of the cell cycle and B-cell survival in the induction of systemic autoimmune and lymphoproliferative diseases.


European Journal of Immunology | 2010

GITR contributes to the systemic adjuvanticity of the Escherichia coli heat-labile enterotoxin

Esther Tamayo; Jorge Postigo; J. González; Maigualida Tamara Fernández-Rey; Marcos Iglesias; Inés Santiuste; Carlo Riccardi; Rino Rappuoli; Giuseppe Del Giudice; Ramón Merino; Jesús Merino

The Escherichia coli heat‐labile enterotoxin (LT) possesses a powerful mucosal and systemic adjuvant effect. However, little is known about the cellular and molecular basis of the immunostimulatory activity of LT at the mucosal level, and even less information is available on the mechanisms underlying its systemic adjuvant activity. In this study, we show that distinct mechanisms are responsible for the parenteral and mucosal adjuvanticity of LT. Indeed, the systemic administration of LT upregulates the expression of glucocorticoid‐induced TNFR‐related protein (GITR), but not other activation markers, in naive T cells. Using WT and GITR‐deficient mice and LT and its enzymatically inactive mutant LTK63 as adjuvants, we show that the induction of GITR expression in T cells accounts for the systemic immunostimulatory capacity of LT, which requires an intact enzymatic activity. In contrast, the mucosal administration of LT does not induce GITR expression on Peyers patche T cells and accordingly no differences are observed in the mucosal adjuvanticity of LT between WT and GITR‐deficient mice. Altogether, our results demonstrate the distinct effect of LT after parenteral administration when compared with the mucosal delivery, and describe a new mechanism of LT adjuvanticity related to its ability to induce the expression of GITR in CD4+ T cells.


PLOS ONE | 2016

Selective Impairment of TH17-Differentiation and Protection against Autoimmune Arthritis after Overexpression of BCL2A1 in T Lymphocytes

Marcos Iglesias; Juan Jesús Augustin; Pilar Álvarez; Inés Santiuste; Jorge Postigo; Jesús Merino; Ramón Merino

The inhibition of apoptotic cell death in T cells through the dysregulated expression of BCL2 family members has been associated with the protection against the development of different autoimmune diseases. However, multiple mechanisms were proposed to be responsible for such protective effect. The purpose of this study was to explore the effect of the T-cell overexpression of BCL2A1, an anti-apoptotic BCL2 family member without an effect on cell cycle progression, in the development of collagen-induced arthritis. Our results demonstrated an attenuated development of arthritis in these transgenic mice. The protective effect was unrelated to the suppressive activity of regulatory T cells but it was associated with a defective activation of p38 mitogen-activated protein kinase in CD4+ cells after in vitro TCR stimulation. In addition, the in vitro and in vivo TH17 differentiation were impaired in BCL2A1 transgenic mice. Taken together, we demonstrated here a previously unknown role for BCL2A1 controlling the activation of CD4+ cells and their differentiation into pathogenic proinflammatory TH17 cells and identified BCL2A1 as a potential target in the control of autoimmune/inflammatory diseases.


Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation | 2009

Leflunomide derivative FK778 inhibits production of antibodies in an experimental model of alloreactive T-B cell interaction.

Ma Ángeles Ramos-Barrón; C. Gómez-Alamillo; Inés Santiuste; Consuelo Agüeros; Lorena San Cosme; Adalberto Benito; Teresa Gimenez; Jesús Merino; Ramón Merino; Manuel Arias


Archive | 2011

Papel de p27KIP1 en el control de la actividad supresora de los linfocitos CD4+CD25+ reguladores en ratones transgénicos para Bcl-2 en linfocitos T

Jorge Postigo; Marcos Iglesias; Esther Tamayo; Inés Santiuste; Ramón Merino; Jesús Merino


Archive | 2010

La sobre-expresión de Bcl-2 incrementa la actividad supresora de los linfocitos CD4+CD25+ reguladores por un mecanismo dependiente de TGFbeta

Marcos Iglesias; Inés Santiuste; Luis Buelta; Jorge Postigo; Jesús Merino; Ramón Merino


Archive | 2008

Interacciones entre reguladores del ciclo celular y de la apóptosis en el desarrollo de autoinmunidad y linfoma

Inés Santiuste; Marcos Iglesias; Luis Buelta; María Sánchez; Esther Tamayo; Jesús Merino; Ramón Merino

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Ramón Merino

Spanish National Research Council

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Luis Buelta

University of Cantabria

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J. González

University of Cantabria

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Regina Marquina

Spanish National Research Council

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