Ioana Roman

Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania

BackgroundThe theoretical, but especially the practical values of identifying the biochemical compounds from the Rosa canina L. fruits are of present interest, this aspect being illustrated by the numerous researches. It was reported that the Rosa canina L. fruit, with its high ascorbic acid, phenolics and flavonoids contents, have antioxidant, antimutagenic and anticarcinogenic effects.This study was performed on order to evaluate the amount of the main phytochemicals (vitamin C, total polyphenols, and total flavonoids) content and their antioxidant activity.ResultsThe results obtained revealed that the average amounts of vitamin C within the studied genotypes were: 360.22 mg/100 g frozen pulp (var. transitoria f. ramosissima, altitude 1250 m) and 112.20 mg/100 g frozen pulp (var. assiensis, altitude 440 m), giving a good correlation between the vitamin C content of the rosehip and the altitude. The total polyphenols content varied from 575 mg/100 g frozen pulp (var. transitoria f. ramosissima) to 326 mg/100 g frozen pulp (var. lutetiana f. fallens). The total flavonoids content showed the highest value for var. assiensis variant 163.3 mg/100 g frozen pulp and the lowest value attributed to var. transitoria f. montivaga 101.3 mg/100 g frozen pulp. The antioxidant activity of eight rose hip extracts from wild Transylvania populations was investigated through DPPH method. The antioxidant activity revealed a good correlation only with vitamin C content and total polyphenols.ConclusionEight Rose hip fruit species were compared taking into consideration the ascorbic acid, total polyphenols, total flavonoids contents and their antioxidant activity. Based on these results, two of the rosehip genotypes that were analysed could be of perspective for these species’ amelioration, due to their content of phytochemicals mentioned above. These varieties are var. transitoria f. ramosissima (Bistrita-Nasaud, Agiesel) and var. transitoria f. montivaga (Bistrita-Nasaud, Salva) which can be used as a potential source of natural antioxidants.

Chemo-mapping and biochemical-modulatory and antioxidant/prooxidant effect of Galium verum extract during acute restraint and dark stress in female rats

Galium verum is a well-known medicinal plant which is used in various pathologies. G. verum extracts are characterized here using chromatography, where among the rich pool of phenolic acids of flavonoids two known anti-stress modulators, chlorogenic acid and rutin are identified in high quantities. Additionally, the extracts are characterized using a series of in vitro assays (EPR, DPPH, TPC and TEAC). Considering the chemical findings, the potential beneficial effects of the G. verum extract are explored here in a living organism exposed to stress induced oxidative damages. Thus, the biochemical-modulatory and antioxidant roles of two doses of G. verum extract are examined in animals exposed to acute restraint and dark stress (S). The animals were divided in groups [control, S, SG1 (exposed to 25 mg G. verum extract), SG2 (50 mg extract)]. Increased levels of lipid peroxidation (TBARS from 4.43 to 8.06 nmol/mL), corticosterone from 0.43 to 1.96 μg/dL and epinephrine from 44.43 to 126.7 μg/mL, as well as decreased antioxidant enzymes activities (SOD/CAT) were observed in the S group. The G. verum extract afforded a near-normal equilibrium within the biochemical parameters of animals exposed to RS, by reducing oxidative damage (TBARS at a 3.73 nmol/mL; CS at 0.90 μg/dL; EP at 63.72 μg/mL) and by restoring the antioxidant balance.

THE INFLUENCE OF FOLIAR FERTILIZATION UPON GROWING AT FIVE APPLE VARIETIES FORM E.U. IN SUPERINTENSIVE CULTURE

SUMMARY In order to reveal the behavior during production p rocess of several apple varieties over Cluj-Napoca tree-growing area, there has been organ ized a competition culture including five varieties of apple. Trees were planted in autumn 20 05, at 3,5/0,9 m distance from one another, thus reaching a thickness of 3174 per ha. The fertilizers utilized in the experience were : F erticare 1, Ferticare 2, Ferticare 3, Ferticare Starter and Power in different concentrat ion amounts. Several aspects regarding trees-size and girth, phe nology of flowering, yielding potential, fruit quality from the point of view of taste and chemical compositions (dry matter and sugar contents, acidity); then, feeding value a nd disease hardiness were taken under study. Apples are very appreciated for their flavor and nu tritional value being used all over the year in the human diet. They are rich in carbohydra tes (glucose, fructose, pectic substances, cellulose, hemicellulose). The organic acids (citri c, malic, succinic, oxalic) are the most important components of the flavor of apples and th ey represent 0.3-0.5 %. The most important vitamins in the apple fruit are: vitamin C (ascorbic acid, around 35 mg/100 g), provitamin A, B etc.