Ioannis Ioannou
Ministry of Interior (Bahrain)
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Featured researches published by Ioannis Ioannou.
Microbial Ecology | 2012
Dimosthenis Chochlakis; Ioannis Ioannou; Vassilios Sandalakis; Theodoros Dimitriou; Nikolaos Kassinis; Byron Papadopoulos; Yannis Tselentis; Anna Psaroulaki
In two surveys conducted from March 1999 to March 2001 and from January 2004 to December 2006, a total of 3,950 ticks (belonging to ten different species) were collected from seven domestic and wild animals (goat, sheep, cattle, dog, fox, hare, and mouflon) from different localities throughout Cyprus. In order to establish their infection rate with Spotted Fever Rickettsiae (SFG), ticks were pooled and tested by polymerase chain reaction targeting gltA and ompA genes, followed by sequencing analysis. When tick pools tested positive, individual ticks were then tested one by one, and of the 3,950 ticks screened, rickettsial DNA was identified in 315 ticks (infection rate, 8%). Five SFG Rickettsiae were identified: Rickettsia aeschlimannii in Hyalomma marginatum marginatum, Rickettsia massiliae in Rhipicephalus turanicus and Rhipicephalus sanguineus, Rickettsia sibirica mongolotimonae in Hyalomma anatolicum excavatum, and a Rickettsia endosymbiont of Haemaphysalis sulcata (later described as Rickettsia hoogstraalii) in Haemaphysalis punctata. Two additional genes, 17xa0kDa and ompB, were targeted to characterize a new genotype of “Candidatus Rickettsia barbariae” genotype in R. turanicus, designated here as “Candidatus Rickettsia barbariae” Cretocypriensis. These results confirm the presence of a spectrum of SFG Rickettsiae on the island. Further studies are necessary to gain better knowledge on the epidemiology of SFG Rickettsiae in Cyprus.
Journal of Comparative Pathology | 2011
Nektarios D. Giadinis; D. Chochlakis; Ioannis Ioannou; M. Kritsepi-Konstantinou; E. Papadopoulos; A. Psaroulaki; H. Karatzias
A 10-month-old ram with fever, inappetence and haemorrhagic diathesis had petechiae and ecchymoses at various body sites and was infested by ticks. Haematological examination revealed pancytopenia, while serum biochemistry indicated hepatic dysfunction. Blood smears were negative for Ehrlichia spp. and other haemoparasites. Paired sera revealed infection by Anaplasma phagocytophilum, but testing by polymerase chain reaction was negative. Treatment with oxytetracycline was effective. This is the first reported clinical case of ovine anaplasmosis in Greece caused by A. phagocytophilum.
Acta Veterinaria Hungarica | 2013
George Filioussis; Ioannis Ioannou; Evanthia Petridou; Maria Avraam; Nektarios D. Giadinis; S. K. Kritas
A major concern with the use of tetracycline against mycoplasmas is the development of resistance. Infections in small ruminants due to tetracyclineresistant Mycoplasma agalactiae strains are becoming a frequent problem worldwide. In the present paper the detection and analysis of three tetracycline-resistant M. agalactiae strains, isolated from infected goats in Cyprus, are reported. The three field isolates were identified as M. agalactiae by polymerase chain reaction (PCR) showing 98% identity to the M. agalactiae PG2 reference strain. Furthermore, they were found sensitive to tylosin, enrofloxacin, spiramycin and lincomycin. In contrast, they were resistant to tetracycline. None of the putative genes [tet(M), tet(O) and tet(S)] that commonly contribute to high-level resistance to tetracycline could be amplified from their genome. Contrarily, the field isolates were found to carry ISMag1, an insertion sequence related to the IS30 family of mobile elements. Although ISMag1 is widely believed to induce high-frequency chromosomal rearrangements resulting in phenotypic changes of microorganisms, its potential role in tetracycline resistance of mycoplasmas requires further studies.
6th World Congress on Mountain Ungulates and 5th International Symposium on Mouflon | 2016
Ioannis Ioannou; Panayiotis Loukopoulos; Chrysostomos I. Dovas; S. Georgiadou; Taxiarhis Chassalevris; Nikolaos Kassinis
10th Panhellenic Virology Conference | 2015
Evangelia Apostolidi; Ioannis Ioannou; Panayiotis Loukopoulos; Chrysostomos I. Dovas
CL Davis and S.Thompson Foundation Diagnostic Exercise Series | 2014
Panayiotis Loukopoulos; Nikolaos Kassinis; Francisco Alejandro Uzal; Chrysostomos I. Dovas; Ioannis Ioannou
Archive | 2013
Panayiotis Loukopoulos; Ioannis Ioannou
Revue De Medecine Veterinaire | 2012
Panayiotis Loukopoulos; Ioannis Ioannou; Nikolaos Kassinis; Soteria Georgiadou; H. Papastephanou; E. Kaldrymidou; Nektarios D. Giadinis
Journal of Comparative Pathology | 2012
Ioannis Ioannou; Nektarios D. Giadinis; Chrysostomos I. Dovas; P. Konstantinou; S. Georgiadou; V. Psychas; E. Kaldrymidou; Panayiotis Loukopoulos
Journal of Comparative Pathology | 2011
Ioannis Ioannou; Nektarios D. Giadinis; Chrysostomos I. Dovas; Konstantinou Panayiotis; Georgiadou Sotiria; V. Psychas; E. Kaldrymidou; Panayiotis Loukopoulos