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Dive into the research topics where Iraj Ghazi is active.

Publication


Featured researches published by Iraj Ghazi.


ChemBioChem | 2013

Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution

Pamela Torres‐Salas; Diana M. Mate; Iraj Ghazi; Francisco J. Plou; Antonio Ballesteros; Miguel Alcalde

Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.


FEBS Letters | 2007

Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family

Nieves López-Cortés; Dolores Reyes-Duarte; Ana Beloqui; Julio Polaina; Iraj Ghazi; Olga V. Golyshina; Antonio Ballesteros; Peter N. Golyshin; Manuel Ferrer

An acetylxylan esterase (R.44), belonging to the carbohydrate esterase family 6 (CE6), retrieved from bovine rumen metagenome was analyzed. Molecular modelling and site‐directed mutagenesis indicated that the enzyme possesses a catalytic triad formed by Ser14, His231 and Glu152. The catalytic Ser and His have been identified in highly conserved sequences GQSX and DXXH in the CE6 family, respectively, and the active‐site glutamate was part of a highly conserved sequence HQGE. This motif is situated near to the so‐called Block III in the CE6 family and its role in catalysis has not been identified so far.


Process Biochemistry | 2008

Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers

Adinarayana Kunamneni; Iraj Ghazi; Susana Camarero; Antonio Ballesteros; Francisco J. Plou; Miguel Alcalde


Journal of Biotechnology | 2007

Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus

Iraj Ghazi; Lucia Fernandez-Arrojo; Humberto García-Arellano; Manuel Ferrer; Antonio Ballesteros; Francisco J. Plou


Journal of Molecular Catalysis B-enzymatic | 2005

Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides

Iraj Ghazi; Aránzazu Gómez de Segura; Lucia Fernandez-Arrojo; Miguel Alcalde; M. Yates; M. Luisa Rojas‐Cervantes; Francisco J. Plou; Antonio Ballesteros


Biotechnology Journal | 2007

Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome.

Manuel Ferrer; Ana Beloqui; Olga V. Golyshina; Francisco J. Plou; Alexander Neef; Tatyana N. Chernikova; Lucia Fernandez-Arrojo; Iraj Ghazi; Antonio Ballesteros; Kieran Elborough; Kenneth N. Timmis; Peter N. Golyshin


Process Biochemistry | 2007

Transformation of maltose into prebiotic isomaltooligosaccharides by a novel α-glucosidase from Xantophyllomyces dendrorhous

Lucia Fernandez-Arrojo; Dolores Marín; Aránzazu Gómez de Segura; Dolores Linde; Miguel Alcalde; Patricia Gutiérrez-Alonso; Iraj Ghazi; Francisco J. Plou; María Fernández-Lobato; Antonio Ballesteros


Journal of Agricultural and Food Chemistry | 2006

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

Iraj Ghazi; Lucia Fernandez-Arrojo; Aránzazu Gómez de Segura; Miguel Alcalde; Francisco J. Plou; Antonio Ballesteros


ChemInform | 2008

Enzymatic Synthesis of Sugar Esters and Oligosaccharides from Renewable Resources

Antonio Ballesteros; Francisco J. Plou; Miguel Alcalde; Manuel Ferrer; Humberto García-Arellano; Dolores Reyes-Duarte; Iraj Ghazi


Journal of Molecular Catalysis B-enzymatic | 2005

Immobilisation of fructosyltransferase from on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides

Iraj Ghazi; A Desegura; Lucia Fernandez-Arrojo; Miguel Alcalde; M. Yates; Maria L. Rojas-Cervantes; Francisco J. Plou; Antonio Ballesteros

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Antonio Ballesteros

Spanish National Research Council

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Francisco J. Plou

Spanish National Research Council

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Lucia Fernandez-Arrojo

Spanish National Research Council

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Miguel Alcalde

Spanish National Research Council

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Manuel Ferrer

Spanish National Research Council

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Aránzazu Gómez de Segura

Spanish National Research Council

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Humberto García-Arellano

Spanish National Research Council

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Ana Beloqui

Spanish National Research Council

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Dolores Linde

Spanish National Research Council

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Dolores Reyes-Duarte

Spanish National Research Council

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