Isaac Peña-Villalobos

Energetic Effects of Pre-hatch Albumen Removal on Embryonic Development and Early Ontogeny in Gallus gallus

Studies on the yolk and albumen content in bird eggs, and the effects of variations in their relative loads in the phenotype of the birds, have revealed multiple consequences at different levels of biological organization, from biochemical traits to behavior. However, little is known about the effect of albumen variation on energetics performance during development and early ontogeny, despite the fact that variation in energy expenditure may have consequences in terms of fitness for both feral and domestic species. In this work, we evaluated experimentally whether variations in the content of albumen of Gallus gallus eggs could generate differences in metabolic rates during embryonic development. Additionally, we assessed changes in the activity of mitochondrial enzymes (cytochrome c oxidase and citrate synthase) in skeletal muscles and liver. Finally, we evaluated the success of hatching of these embryos and their metabolic rates (MR) post-hatching. The results revealed a significant reduction in MR in the last fifth of embryonic life, and reduced catabolic activities in the skeletal muscle of chicks hatched from albumen-removed eggs. However, the same group demonstrated an increase in catabolic activity in the liver, suggesting the existence of changes in energy allocation between tissues. Besides, we found a decrease in hatching success in the albumen-removed group, suggesting a negative effect of the lower albumen content on eggs, possibly due to lower catabolic activities in skeletal muscle. We also found a compensatory phenomenon in the first week after hatching, i.e., birds from albumen-removed eggs did not show a decrease in MR either at thermoneutral temperatures or at 10°C, compared to the control group. Collectively, our data suggest that a reduction in albumen may generate a trade-off between tissue metabolic activities, and may explain the differences in metabolic rates and hatching success, supporting the immediate adaptive response (IAR) hypothesis.

Metabolic cost of osmoregulation in a hypertonic environment in the invasive African clawed frog Xenopus laevis

ABSTRACT Studies of aquatic invertebrates reveal that salinity affects feeding and growth rates, reproduction, survival, and diversity. Little is known, however, about how salinity impacts the energy budget of vertebrates and amphibians in particular. The few studies focused on this topic in vertebrates suggest that the ingestion of salts and the resulting osmoregulatory activity is energetically expensive. We analyzed the effect of saline acclimation on standard metabolic rates (SMR) and the activities of metabolic enzymes of internal organs and osmoregulatory variables (plasma osmolality and urea plasma level) in females of Xenopus laevis by means of acclimating individuals to an isosmotic (235 mOsm NaCl; ISO group) and hyper-osmotic (340 mOsm NaCl; HYP group) environment for 40 days. After acclimation, we found that total and mass-specific SMR was approximately 80% higher in the HYP group than those found in the ISO group. These changes were accompanied by higher citrate synthase activities in liver and heart in the HYP group than in the ISO group. Furthermore, we found a significant and positive correlation between metabolic rates and plasma urea, and citrate synthase activity in liver and heart. These results support the notion that the cost of osmoregulation is probably common in most animal species and suggest the existence of a functional association between metabolic rates and the adjustments in osmoregulatory physiology, such as blood distribution and urea synthesis. Summary: A significant increase in energy expenditure of Xenopus laevis exposed to hyperosmotic conditions is associated with urea concentration and a long-term effect of increasing the metabolic enzyme activities of liver and heart.

Aprovechamiento humano de aves marinas durante el Holoceno medio en el litoral árido del norte de Chile

Se identifico y analizo la osteofauna aviar del sitio arqueologico Copaca 1, ubicado en el litoral arido de la segunda Region de Chile. La muestra estuvo compuesta por 685 fragmentos extraidos desde nueve capas con dataciones que cubren un rango entre 8000 - 5000 anos cal. a.p. Los resultados indican que el ensamble de aves marinas encontrado en este sitio conserva actualmente su rango de distribucion en la zona de estudio, siendo dominantes los ordenes Suliformes, Procellariiformes y Pelecaniformes. Los analisis sugieren un aprovechamiento principalmente sobre Puffinus sp., Pelecanus thagus y Phalacrocorax sp., identificandose un minimo numero de individuos de 74 aves. Ademas, se estimo la masa corporal utilizable aportada por cada taxon y se determino una representacion diferencial de elementos anatomicos apendiculares junto con diversos grados de fraccionamiento y marcas culturales, que indicarian obtencion de alimento y elaboracion de artefactos. Nuestros resultados apoyan el aprovechamiento de aves marinas durante el Holoceno medio en la costa suroccidental de Sudamerica.

Coping with Salt Water Habitats: Metabolic and Oxidative Responses to Salt Intake in the Rufous-Collared Sparrow

Many physiological adjustments occur in response to salt intake in several marine taxa, which manifest at different scales from changes in the concentration of individual molecules to physical traits of whole organisms. Little is known about the influence of salinity on the distribution, physiological performance, and ecology of passerines; specifically, the impact of drinking water salinity on the oxidative status of birds has been largely ignored. In this study, we evaluated whether experimental variations in the salt intake of a widely-distributed passerine (Zontotrichia capensis) could generate differences in basal (BMR) and maximum metabolic rates (Msum), as well as affect metabolic enzyme activity and oxidative status. We measured rates of energy expenditure of birds after 30-d acclimation to drink salt (SW) or tap (fresh) water (TW) and assessed changes in the activity of mitochondrial enzymes (cytochrome c oxidase and citrate synthase) in skeletal muscle, heart, and kidney. Finally, we evaluated the oxidative status of bird tissues by means of total antioxidant capacity (TAC) and superoxide dismutase activities and lipid oxidative damage (Malondialdehyde, MDA). The results revealed a significant increase in BMR but not Msum, which resulted in a reduction in factorial aerobic scope in SW- vs. TW-acclimated birds. These changes were paralleled with increased kidney and intestine masses and catabolic activities in tissues, especially in pectoralis muscle. We also found that TAC and MDA concentrations were ~120 and ~400% higher, respectively in the liver of animals acclimated to the SW- vs. TW-treatment. Our study is the first to document changes in the oxidative status in birds that persistently drink saltwater, and shows that they undergo several physiological adjustments that range that range in scale from biochemical capacities (e.g., TAC and MDA) to whole organism traits (e.g., metabolic rates). We propose that the physiological changes observed in Z. capensis acclimated to saltwater could be common phenomena in birds and likely explain selection of prey containing little salt and habitats associated with low salinity.

Discriminación de taxones de aves a través del análisis comparativo de la microestructura de plumas: una aplicación en el sitio tulán 122, Puna de Atacama, Chile

Results of analysis from feathers recovered in the Early Formative (ca. 2700 B.P.) Tulan-122 site are presented. The site is located in Quebrada Tulan on the eastern slope of the Puna de Atacama. Macroscopic features (coloration and size) of taxidermic and zoorachaeological samples were compared. A protocol for the preparation of feather barbules was performed and the nodes were studied by using amplification (1400X). The genus Sicalis (Boie 1828) was identified. Results of the feather and bones are compared in order to discuss the taphonomic variables and interpretation of the use of birs in the site.

Hyperbaric Oxygen Increases Stem Cell Proliferation, Angiogenesis and Wound-Healing Ability of WJ-MSCs in Diabetic Mice

Hyperbaric oxygen therapy (HBOT) is effective for the medical treatment of diverse diseases, infections, and tissue injury. In fact, in recent years there is growing evidence on the beneficial effect of HBOT on non-healing ischemic wounds. However, there is still yet discussion on how this treatment could benefit from combination with regenerative medicine strategies. Here we analyzed the effects of HBOT on three specific aspects of tissue growth, maintenance, and regeneration: (i) modulation of adult rodent (Mus musculus) intestinal stem cell turnover rates; (ii) angiogenesis dynamics during the development of the chorio-allantoic membrane (CAM) in Gallus gallus embryos; (iii) and wound-healing in a spontaneous type II diabetic mouse model with a low capacity to regenerate skin. To analyze these aspects of tissue growth, maintenance, and regeneration, we used HBOT alone or in combination with cellular therapy. Specifically, Wharton Jelly Mesenchymal Stem cells (WJ-MSC) were embedded in a commercial collagen-scaffold. HBOT did not affect the metabolic rate of adult mice nor of chicken embryos. Notwithstanding, HBOT modified the proliferation rate of stem cells in the mice small intestinal crypts, increased angiogenesis in the CAM, and improved wound-healing and tissue repair in diabetic mice. Moreover, our study demonstrates that combining stem cell therapy and HBOT has a collaborative effect on wound-healing. In summary, our data underscore the importance of oxygen tension as a regulator of stem cell biology and support the potential use of oxygenation in clinical treatments.