Ismail Ozmen

Spinal Morphine Administration Reduces the Fatty Acid Contents in Spinal Cord and Brain by Increasing Oxidative Stress

It is well known that oxidative stress damages bimolecules such as DNA and lipids. No study is available on the morphine-induced oxidative damage and fatty acids changes in brain and spinal tissues. The aim of this work was to determine the effects of morphine on the concentrations and compositions of fatty acid in spinal cord segments and brain tissues in rabbits as well as lipid peroxidation (LP) and glutathione (GSH) levels in cortex brain.Twelve New Zealand albino rabbits were used and they were randomly assigned to two groups of 6 rabbits each. First group used as control although morphine administrated to rats in second group. Cortex brain and (cervical, thoracic, lumbar) samples were taken.The fatty acids between n:18.0 and 21.0 were present in spinal cord sections and n:10 fatty acids in control animals were present in the brain tissues. Compared to n:20.0–24.0 fatty acids in spinal cord sections and 8.0 fatty acids in the brain tissues of drug administered animals. The concentration and composition of the fatty acid methyl esters in spinal cord and brain tissues was decreased by morphine treatments. LP levels in the cortex brain were increased although GSH levels were decreased by the morphine administration.In conclusion, unsaturated fatty acids contents in brain and spinal cord sections and GSH were reduced by administrating spinal morphine although oxidative stress as LP increased. The inhibition oxidative damage may be a useful strategy for the development of a new protection for morphine administration as well as opiate abuse.

Synthesis, Characterization, Thermal Behavior, and DNA-Cleaving Studies of Cyano-Bridged Nickel(II)Copper(II) Complexes of 4-(Pyridin-2-ylazenyl)resorcinol

We present here the syntheses of a mononuclear CuII complex and two polynuclear CuIINiII complexes of the azenyl ligand, 4‐(pyridin‐2‐ylazenyl)resorcinol (HL; 1). The reaction of HL (1) and copper(II) perchlorate with KCN gave a mononuclear complex [CuL(CN)] (4). Using 4, one pentanuclear complex, [{CuL(NC)}4Ni](ClO4)2 (5) and one trinuclear complex, [{CuL(CN)}2NiL]ClO4 (6), were prepared and characterized by elemental analyses, magnetic susceptibility, molar conductance, IR, and thermal analysis. Stoichiometric and spectral results of the mononuclear CuII complex indicated that the metal/ligand/CN ratio was 1 : 1 : 1, and the ligand behaved as a tridentate ligand forming neutral metal chelates through the pyridinyl and azenyl N‐, and resorcinol O‐atom. The interaction between the compounds (the ligand 1, its NiII and CuII complexes without CN, i.e., 2 and 3, and its complexes with CN, 4–6) and DNA has also been investigated by agarose gel electrophoresis. The pentanuclear Cu4Ni complex (5) with H2O2 as a co‐oxidant exhibited the strongest DNA‐cleaving activity.

MONO-, TRINUCLEAR NICKEL(II) AND COPPER(II) DIOXIME COMPLEXES: SYNTHESIS, CHARACTERIZATION, CATECHOLASE AND CATALASE-LIKE ACTIVITIES, DNA CLEAVAGE STUDIES

The synthesis and molecular structures of six new mono- and trinuclear complexes [M1L 2 {M2(phen)} 2 ](ClO 4 ) 2 [phen = 1,10-phenanthroline, H 2 L = dioxime ligand, M1, M2 =Ni(II) and Cu(II)] of the ligand, 4-(4-methylphenylamino)biphenylglyoxime are reported. Structural assignments are supported by a combination of FT-IR, elemental analyses, ICP-OES, magnetic susceptibility and molar conductivity studies. Furthermore these complexes were each tested for their ability to catalyze the oxidation of catechol substrates to quinone with dioxygen at 25oC and disproportionation of hydrogen peroxide in the presence of the added base imidazole. The catalytic results indicated that the heterotrinuclear Cu(II)Ni(II)Cu(II) complex (5) shows greater catecholase activity. On the other hand the trinuclear complexes display efficiency in disproportion reactions of hydrogen peroxide producing water and dioxygen in catalase-like activity and homotrinuclear Cu(II) (6) complex is more active towards catalytic decomposition of hydrogen peroxide. The interaction between these compounds with DNA has also been investigated by agarose gel electrophoresis, the trinuclear copper(II) complexes (5, 6) with H 2 O 2 as a cooxidant exhibited the strongest cleaving activity.

Synthesis of new calix[4]arene amide derivatives and investigation of their DNA cleavage activity

This study comprises the synthesis of new p-tert-butylcalix[4]arene with different amide functional groups and summarises an investigation of their DNA cleavage activities. The structural investigations of the synthesised compounds were examined by FTIR, 1H NMR, 13C NMR, elemental analysis and FAB-MS techniques. The interaction between these compounds and pBR322 plasmid DNA has been investigated via agarose gel electrophoresis and, according to the results, compounds 5, 7, 8 and 13 exhibit efficient DNA cleavage activity. In the electrophoresis images of 5, 7 and 8, Form IV which is small DNA fragment was observed in addition to supercoiled Form I, open circular Form II and linear Form III.

Synthesis, Characterization, Electrochemical Behavior, and Biological Studies of 1-p-Diphenylmethane-2-hydroxyimino-2-(arylamino)-1-ethanone Copper(II) and Ruthenium(III) Complexes

New copper and ruthenium mononuclear complexes of the type [ML2(H2O)X] [X = H2O for M = Cu(II) and X = Cl for M = Ru(III)] have been prepared from 1-p-diphenyl- methane-2-hydroxyimino-2-(4-chloroanilino)-1-ethanone (HL1) and 1-p-diphenylmethane-2-hydroxyimino-2-(4-toluidino)-1-etha- none (HL2). The complexes were characterized by elemental analyses, magnetic susceptibility, molar conductance, IR, thermal analysis, and cyclic voltammetry. Stoichiometric and spectral results of the metal complexes indicated that the metal:ligand ratios in the complexes were found to be 1:2 and the ligands behave as a bidentate ligand forming neutral metal chelates through the carbonyl and oxime oxygen. The electrochemical behavior of the ligands and their complexes were obtained by cyclic voltammetry. The interaction between these complexes with DNA has also been investigated by agarose gel electrophoresis. The copper(II) complexes (3 and 4) with H2O2 as a co-oxidant exhibited strongest cleaving activity. Moreover, catalytic activities of the complexes for the disproportionation of hydrogen peroxide were also investigated in the presence of imidazole.

4,4'-(DIPHENYLMETHANE-4,4'-DIYLDINITRILO)DIPENTAN-2-ONE AND 4,4'-(DIPHENYLETHER-4,4'-DIYLDINITRILO)DIPENTAN-2-ONE LIGANDS AND THEIR Ni(II), Cu(II) COMPLEXES: SYNTHESIS, CHARACTERIZATION, DNA CLEAVAGE STUDIES

The synthesis of two new ligands 4,4’-(diphenylmethane-4,4’-diyldinitrilo)dipentan-2-one (H 2 L1) and 4,4’-(diphenylether-4,4’-diyldinitrilo)dipentan-2one (H 2 L2) is reported. The ligands have two acetylacetone subunits separated by diphenylmethane or diphenylether group. The ligands form stable dinuclear complexes with Ni(II) and Cu(II) ions. Ligands and their complexes were characterized by elemental analysis, m.p., IR, molar conductivity and magnetic moment measurements. The interaction between these compounds with DNA has also been investigated by agarose gel electrophoresis, we found that the nickel(II) and copper(II) complexes can cleave supercoiled pBR322 DNA to nicked and linear forms. The copper(II) complexes with H 2 O 2 as a cooxidant exhibited the strongest cleaving activity. The free ligands were also characterized by 1H NMR spectra. The IR of the free ligands and their complexes are compared and discussed. The extraction ability of the ligands have been examined by the liquid–liquid extraction of selected transition metal [Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, Hg2+ and Pb2+] cations. The ligands show strong binding ability toward mercury(II) ion.