Italo Capurro V

Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile

: Detection of HPV was 40% in cases without histologicallesion, 88% in low grade lesions, 89% in high grade lesions (HGL) and 93.5% in invasive carcino-ma. Of all HPV positive cases, 89.5% were classified as high risk and only a 4.9% of HPV caseswere of low risk type. Six percent of cases had multiple infections. Distribution of viral genotypesaccording to RFLP was: HPV33 (25.3%), 16 (18.7%), 52 (13.3%), 31 (12%), 35 (6.6%), 18(2.7%).

Estudio comparativo de detección del virus papiloma humano (VPH) en muestras citológicas y biopsias de cuello uterino

Background: The DNA quality for the detection and typification of Human Papilloma Virus (HPV) varies according to the type of sample in which it is studied. This may affect the sensitivity and specificity of the method employed. Aim: To study the yield and specificity of HPV detection and typification in uterine cervical samples obtained by cervical brushing fresh frozen and formalin fixed tissue. Material and methods: Cytological, fresh frozen and fixed tissue samples from 44 patients (nine with low grade lesions and 35 with high grade lesions) were studied. Nested polymerase chain reaction for genes E6/E7 was used to typify HPV groups as low risk or high risk. Results: Of all the cytological samples obtained by brushing 84% of fixed samples and 43% of fresh frozen samples were positive for HPV. The yields were significantly different when comparing brushing with fixed tissue or fresh frozen tissue and fixed tissue with fresh frozen tissue (p <0.05). The frequency of high risk HPV fluctuated from 41% in fresh frozen tissue to 98% in cytological samples. Low risk HPV was detected in 16% of fresh frozen tissue and 68% of cytological samples. A mixed infection was detected in 66%, 41% and 14% of cytological, fresh frozen and fixed tissue samples respectively. Conclusions: Cytological samples obtained by brushing had the highest yield for the detection of cervical infection with HPV (Rev Med Chile 2005; 133: 639-44). (Key Words: Cytological techniques; Cytopathology; Papillomavirus, human)

Detección y tipificación de virus papiloma humano en lesiones preneoplásicas del cuello uterino mediante PCR-RFLP

. Los genotiposde HPV que infectan el tracto genital femenino, sehan clasificado en 2 grupos: de bajo riesgo (BR) y dealto riesgo (AR) segun el potencial oncogenico parael desarrollo de lesiones preneoplasicas o neoplasi-cas. Los genotipos de bajo riesgo (BR) mas frecuen-tes son los HPV: 6, 11, 43 y 44 y se asocian concondilomas o neoplasias cervicales intraepitelialesde bajo grado (LBG). En el grupo de alto riesgo (AR)15 tipos de HPV han sido identificados (HPV 16, 18,31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73 y 82),detectados principalmente en neoplasia cervicalintraepitelial de alto grado (LAG) y carcinomas

Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino

The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.

Genotipificación del virus papiloma humano en mujeres con adenocarcinoma cervical de la Región de La Araucanía-Chile

Resumen El virus papiloma humano (VPH) es el principal factor causal del cancer cervicouterino (CCU). Asi, de-tectar y genotipi fi car el VPH es importante para conocer la frecuencia de los genotipos presentes en la region. En este trabajo se estudiaron 44 biopsias de adenocarcinoma cervical (ACC). Para la deteccion del VPH se empleo una reaccion de polimerasa en cadena anidada dirigida al gen L1 (RPCL1), para la genotipifi cacion viral se utilizaron enzimas de restriccion ( Rsa I, Dde I, Pst I) y secuenciacion. Se detecto ADN viral mediante RPCL1 anidada en 100% de las biopias. Se logro tipifi car 38/44 casos: 81,6% VPH 16; 13,2% VPH 18; 2,6% VPH 33 y 2,6% VPH 18/33. Conclusiones: La metodologia fue exitosa para identifi car el tipo viral en 86% de las biopsias. Se observo una estrecha asociacion ACC-VPH, especialmente con el tipo viral 16, detectado en 81,6% de los casos tipifi cados. Figura 3. RPCL1 anidada- secuenciacion. Se muestra el cromatograma de una secuencia representativa.

Inestabilidad microsatelital en lesiones preneoplásicas y neoplásicas del cuello uterino: Correlación con el genotipo del virus papiloma humano

Biopsy samples of cervical lesions were studied. Sixteen hadlow grade lesions, 22 had high grade lesions and 28 had an epidermoid cancer. Viral types wereidentified with polymerase chain reaction, dot-blot hybridization and restriction fragment lengthpolymorphism. MSI was determined using a panel of eight highly informative microsatellites.