Ivan Muñoz

Ranking potential impacts of priority and emerging pollutants in urban wastewater through life cycle impact assessment

Life cycle impact assessment (LCIA), a feature of the Life cycle assessment (LCA) methodology, is used in this work outside the LCA framework, as a means to quantify the potential environmental impacts on ecotoxicity and human toxicity of wastewater containing priority and emerging pollutants. In order to do this, so-called characterisation factors are obtained for 98 frequently detected pollutants, using two characterisation models, EDIP97 and USES-LCA. The applicability of this methodology is shown in a case study in which wastewater influent and effluent samples from a Spanish wastewater treatment plant located in the Mediterranean coast were analysed. Characterisation factors were applied to the average concentration of each pollutant, obtaining impact scores for different scenarios: discharging wastewater to aquatic recipient, and using it for crop irrigation. The results show that treated wastewater involves a substantially lower environmental impact when compared to the influent, and pharmaceuticals and personal care products (PPCPs) are very important contributors to toxicity in this wastewater. Ciprofloxacin, fluoxetine, and nicotine constitute the main PPCPs of concern in this case study, while 2,3,7,8-TCDD, Nickel, and hexachlorobenzene are the priority pollutants with highest contribution. Nevertheless, it must be stressed that the new characterisation factors are based on very limited data, especially with regard to toxicology, and therefore they must be seen as a first screening to be improved in the future when more and higher quality data is available.

Life Cycle Assessment of urban wastewater reuse with ozonation as tertiary treatment: A focus on toxicity-related impacts

Life Cycle Assessment has been used to compare different scenarios involving wastewater reuse, with special focus on toxicity-related impact categories. The study is based on bench-scale experiments applying ozone and ozone in combination with hydrogen peroxide to a wastewater effluent from a Spanish sewage treatment plant. Two alternative characterisation models have been used to account for toxicity of chemical substances, namely USES-LCA and EDIP97. Four alternative scenarios have been assessed: wastewater discharge plus desalination supply, wastewater reuse without tertiary treatment, wastewater reuse after applying a tertiary treatment consisting on ozonation, and wastewater reuse after applying ozonation in combination with hydrogen peroxide. The results highlight the importance of including wastewater pollutants in LCA of wastewater systems assessing toxicity, since the contribution of wastewater pollutants to the overall toxicity scores in this case study can be above 90%. Key pollutants here are not only heavy metals and other priority pollutants, but also non-regulated pollutants such as pharmaceuticals and personal care products. Wastewater reuse after applying any of the tertiary treatments considered appears as the best choice from an ecotoxicity perspective. As for human toxicity, differences between scenarios are smaller, and taking into account the experimental and modelling uncertainty, the benefits of tertiary treatment are not so clear. From a global warming potential perspective, tertiary treatments involve a potential 85% reduction of greenhouse gas emissions when compared with desalination.

Molecular Characterization of Ypi1, a Novel Saccharomyces cerevisiae Type 1 Protein Phosphatase Inhibitor

The Saccharomyces cerevisiae open reading frame YFR003c encodes a small (155-amino acid) hydrophilic protein that we identified as a novel, heat-stable inhibitor of type 1 protein phosphatase (Ypi1). Ypi1 interacts physically in vitro with both Glc7 and Ppz1 phosphatase catalytic subunits, as shown by pull-down assays. Ypi1 inhibits Glc7 but appears to be less effective toward Ppz1 phosphatase activity under the conditions tested. Ypi1 contains a 48RHNVRW53 sequence, which resembles the characteristic consensus PP1 phosphatase binding motif. A W53A mutation within this motif abolishes both binding to and inhibition of Glc7 and Ppz1 phosphatases. Deletion of YPI1 is lethal, suggesting a relevant role of the inhibitor in yeast physiology. Cells overexpressing Ypi1 display a number of phenotypes consistent with an inhibitory role of this protein on Glc7, such as decreased glycogen content and an increased growth defect in a slt2/mpk1 mitogen-activated protein kinase-deficient background. Taking together, these results define Ypi1 as the first inhibitory subunit of Glc7 identified in budding yeast.

Analysis of water use impact assessment methods (part A): evaluation of modeling choices based on a quantitative comparison of scarcity and human health indicators

PurposeIn the past decade, several methods have emerged to quantify water scarcity, water availability and the human health impacts of water use. It was recommended that a quantitative comparison of methods should be performed to describe similar impact pathways, namely water scarcity and human health impacts from water deprivation. This is precisely the goal of this paper, which aims to (1) identify the key relevant modeling choices that explain the main differences between characterization models leading to the same impact indicators; (2) quantify the significance of the differences between methods, and (3) discuss the main methodological choices in order to guide method development and harmonization efforts.MethodsThe modeling choices are analysed for similarity of results (using mean relative difference) and model response consistency (through rank correlation coefficient). Uncertainty data associated with the choice of model are provided for each of the models analysed, and an average value is provided as a tool for sensitivity analyses.ResultsThe results determined the modeling choices that significantly influence the indicators and should be further analysed and harmonised, such as the regional scale at which the scarcity indicator is calculated, the sources of underlying input data and the function adopted to describe the relationship between modeled scarcity indicators and the original withdrawal-to-availability or consumption-to-availability ratios. The inclusion or exclusion of impacts from domestic user deprivation and the inclusion or exclusion of trade effects both strongly influence human health impacts. At both midpoint and endpoint, the comparison showed that considering reduced water availability due to degradation in water quality, in addition to a reduction in water quantity, greatly influences results. Other choices are less significant in most regions of the world. Maps are provided to identify the regions in which such choices are relevant.ConclusionsThis paper provides useful insights to better understand scarcity, availability and human health impact models for water use and identifies the key relevant modeling choices and differences, making it possible to quantify model uncertainty and the significance of these choices in a specific regional context. Maps of regions where these specific choices are of importance were generated to guide practitioners in identifying locations for sensitivity analyses in water footprint studies. Finally, deconstructing the existing models and highlighting the differences and similarities has helped to determine building blocks to support the development of a consensual method.

Environmental and human health risk assessment of organic micro-pollutants occurring in a Spanish marine fish farm.

In this work the risk posed to seawater organisms, predators and humans is assessed, as a consequence of exposure to 12 organic micro-pollutants, namely metronidazole, trimethoprim, erythromycin, simazine, flumequine, carbaryl, atrazine, diuron, terbutryn, irgarol, diphenyl sulphone (DPS) and 2-thiocyanomethylthiobenzothiazole (TCMTB). The risk assessment study is based on a 1-year monitoring study at a Spanish marine fish farm, involving passive sampling techniques. The results showed that the risk threshold for irgarol concerning seawater organisms is exceeded. On the other hand, the risk to predators and especially humans through consumption of fish is very low, due to the low bioconcentration potential of the substances assessed.

LCA application to integrated waste management planning in Gipuzkoa (Spain).

Goal, Scope and BackgroundGipuzkoa is a department of the Vasque Country (Spain) with a population of about 700,000 people. By the year 2000 approximately 85% of municipal solid waste in this area was managed by landfilling, and only 15% was recycled. Due to environmental law restrictions and landfill capacity being on its limit, a planning process was initiated by the authorities. LCA was used, from an environmental point of view, to assess 7 possible scenarios arising from the draft Plan for the 2016 time horizon.Main FeaturesIn each scenario, 9 waste flows are analysed: rest waste, paper and cardboard, glass containers, light packaging, organic-green waste, as well as industrial/commercial wood, metals and plastics, and wastewater sludge. Waste treatments range from recycling to energy recovery and landfilling.ResultsRecycling of the waste flows separated at the source (paper and cardboard, glass, light packaging, organic-green waste, wood packaging, metals and plastics) results in net environmental benefits caused by the substitution of primary materials, except in water consumption. These benefits are common to the 7 different scenarios analysed. However, some inefficiencies are detected, mainly the energy consumption in collection and transport of low density materials, and water consumption in plastic recycling. The remaining flows, mixed waste and wastewater sludge, are the ones causing the major environmental impacts, by means of incineration, landfilling of partially stabilised organic material, as well as thermal drying of sludge. With the characterisation results, none of the seven scenarios can be clearly identified as the most preferable, although, due to the high recycling rates expected by the Plan, net environmental benefits are achieved in 9 out of 10 impact categories in all scenarios when integrated waste management is assessed (the sum of the 9 flows of waste). Finally, there are no relevant differences between scenarios concerning the number of treatment plants considered. Nevertheless, only the effects on transportation impacts were assessed in the LCA, since the plant construction stage was excluded from the system boundaries.ConclusionsThe results of the study show the environmental importance of material recycling in waste management, although the recycling schemes assessed can be improved in some aspects. It is also important to highlight the environmental impact of incineration and landfilling of waste, as well as thermal drying of sludge using fossil fuels. One of the main findings of applying LCA to integrated waste management in Gipuzkoa is the fact that the benefits of high recycling rates can compensate for the impacts of mixed waste and wastewater sludge.Recommendations and OutlookAlthough none of the scenarios can be clearly identified as the one having the best environmental performance, the authorities in Gipuzkoa now have objective information about the future scenarios, and a multidisciplinary panel could be formed in order to weight the impacts if necessary. In our opinion, LCA was successfully applied in Gipuzkoa as an environmental tool for decision making.

Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis

Unlike most other organisms, the essential five-step coenzyme A biosynthetic pathway has not been fully resolved in yeast. Specifically, the genes encoding the phosphopantothenoylcysteine decarboxylase (PPCDC) activity still remain unidentified. Sequence homology analyses suggest three candidates-Ykl088w, Hal3 and Vhs3-as putative PPCDC enzymes in Saccharomyces cerevisiae. Notably, Hal3 and Vhs3 have been characterized as negative regulatory subunits of the Ppz1 protein phosphatase. Here we show that YKL088w does not encode a third Ppz1 regulatory subunit, and that the essential roles of Ykl088w and the Hal3 and Vhs3 pair are complementary, cannot be interchanged and can be attributed to PPCDC-related functions. We demonstrate that while known eukaryotic PPCDCs are homotrimers, the active yeast enzyme is a heterotrimer that consists of Ykl088w and Hal3/Vhs3 monomers that separately provides two essential catalytic residues. Our results unveil Hal3 and Vhs3 as moonlighting proteins involved in both CoA biosynthesis and protein phosphatase regulation.

Identification of multicopy suppressors of cell cycle arrest at the G1–S transition in Saccharomyces cerevisiae

Inactivation of HAL3 in the absence of SIT4 function leads to cell cycle arrest at the G1–S transition. To identify genes potentially involved in the control of this phase of the cell cycle, a screening for multicopy suppressors of a conditional sit4 hal3 mutant (strain JC002) has been developed. The screening yielded several genes known to perform key roles in cell cycle events, such as CLN3, BCK2 or SWI4, thus proving its usefulness as a tool for this type of studies. In addition, this approach allowed the identification of additional genes, most of them not previously related to the regulation of G1–S transition or even without known function (named here as VHS1‐3, for viable in a hal3 sit4 background). Several of these gene products are involved in phospho‐dephosphorylation processes, including members of the protein phosphatase 2A and protein phosphatases 2C families, as well as components of the Hal5 protein kinase family. The ability of different genes to suppress sit4 phenotypes (such as temperature sensitivity and growth on non‐fermentable carbon sources) or to mimic the functions of Hal3 was evaluated. The possible relationship between the known functions of these suppressor genes and the progress through the G1–S transition is discussed. Copyright

Integration of Environmental and Economic Performance of Processes. Case Study on Advanced Oxidation Processes for Wastewater Treatment

Abstract The aim of this paper is to introduce an easy-to-use environmental-economic index (EEI) which can be used for comparative purposes when assessing processes and services. The EEI differs from traditional ratiobased Eco-efficiency indicators, since it is defined instead as the sum of two components, one corresponding to environmental performance and the other to economic performance. In order to show the calculation of this index and its potential usefulness, it is used in an example where several Advanced Oxidation Processes (AOPs) for wastewater treatment are compared based on their life-cycle greenhouse gas emissions and life-cycle cost. The options assessed are: solar-driven photo-Fenton, lamp-driven photo-Fenton, and ozonation. Solar-driven photo- Fenton appears as the preferable option from an integrated environmental-economic point of view, if the environmental and economic dimensions are equally weighed. The case study shows that EEI captures, in a single score, both environmental and economic information from a given set of alternative options, allowing us to assess their relative performance in a simple way.

Accounting for greenhouse gas emissions from the degradation of chemicals in the environment

PurposeThe degradation of chemicals in the environment is often excluded from life cycle assessment (LCA) studies. This paper describes a method to account for greenhouse gas (GHG) emissions from degradation, namely carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O).Materials and methodsA multi-media fate model is proposed to estimate the distribution and degradation of chemicals released to the environment. The environmental distribution, along with emission factors and assumptions for each compartment (air, water, soil and sediments), is used to calculate emissions of CO2, CH4 and N2O associated with the initial release of a chemical to either the air, water or soil compartments. These emissions, along with global warming potentials (GWP), are converted to CO2 equivalents from degradation (GWPdeg). The method is described and evaluated with nine organic chemicals.Results and discussionApplying the proposed method to the example chemicals shows that all the end products (CO2, CH4 and N2O) have the potential to significantly contribute to GWPdeg, depending on the chemicals’ composition and environmental fate. These emissions, taken to represent the end-of-life stage of these nine chemicals, are shown to be significant when compared with the corresponding cradle-to-gate emissions. For three of the nine chemicals, the degradation emissions in the environment exceeded the cradle to gate phase.ConclusionsThe method enables the accounting of GHG emissions that are currently neglected in many LCA and carbon footprint studies. The model has been parameterized for a generic environmental scenario, but it can be adapted for more site-specific conditions. Uncertainty in this method is associated to the potential lack of chemical-specific data to use in fate modelling, the generic evaluative environment used in fate models and the uncertainty associated with emission factors. A Monte Carlo analysis on the latter shows that the highest uncertainty is expected for chemicals where N2O emissions are the main contributor to GWPdeg.