Ivica Milosavljevic

RESVERATROL REDUCES BLOOD PRESSURE, CHANGES OF ANTIOXIDANT ENZYME ACTIVITY AND HISTOLOGICAL PARAMETERS IN EXPERIMENTAL MODEL OF MALIGNANT HYPERTENSION: PP.29.171

Objective: Spontaneously hypertensive rats (SHR) treated with NO syntase inhibitor L-NAME, develop malignant hypertension. This study designed to examine the role of chronic administration of resveratrol on blood pressure, activity of antioxidant enzyme and histological parameters in model of malignant hypertension. Design and Methods: Experiments were performed in adult female SHR, weight 250 g. Two groups of 22 SHR each involved: L-NAME group treated with L-NAME (10 mg/kg per os) and COMB group treated with L-NAME (10 mg/kg per os) and resveratrol (10 mg/kg by gavage). Mean arterial pressure (MAP) and aortal blood flow (ABF) were measured at the end of the 4 weeks treatment period and aortal vascular resistance (AVR) was calculated. Activity of antioxidant enzyme, reduced glutathione (GR) and glutathione peroxidase (GSH-Px) were estimated in erythrocyte. The aorta was preceded for histopathological analysis. Results: The mean arterial pressure after treatment significantly dropped in COMB (157.10 ± 7.49 mmHg) compared L-NAME (182.79 ± 2.16 mmHg). Treatment reduced aortal blood flow that leads to significantly decrease of aortal vascular resistance in COMB (35.54 ± 3.31 mmHg min 100 g/ml) vs L-NAME (45.54 ± 3.06 mmHg min 100 g/ml). Resveratrol in COMB increased activity of GR (30951.23 ± 10253.54 μmol NADPH/min/g Hb) and GSH-Px (9.23 ± 2.10 μmol NADPH/min/g Hb) vs L-NAME (24034.13 ± 5743.76 μmol NADPH/min/g Hb; 3.88 ± 1.14 μmol NADPH/min/g Hb). In model of malignant hypertension histological structure of aorta showed disintegration of endothelial, marked atrophic in tunicae media and necrotic modification in smooth muscle cells. Treatment with resveratrol preserved endothelial and reduced morphological changes in tunica media and smooth muscle cells of aorta. Histopatological score of aorta was significantly reduced in the COMB group (2.50 ± 0.13) compared to L-NAME (3.31 ± 0.12). Conclusions: These results suggest that resveratrol reduced mean arterial pressure and aortal blood flow, probably by decreasing peripheral vascular resistance. Chronic treatment with resveratrol in model of malignant hypertension significantly increased activity of antioxidant enzymes, and reduced morphological changes of aorta. Thus, resveratrol possess antioxidative and antihypertensive effects.

Knowledge management system for clinical decision support — Application in cardiology

Clinical decision support systems have been recognized as promising tools for influencing healthcare provider performance to improve and streamline the quality of healthcare delivery. In this paper we present the knowledge management module, developed as part of KardioNet system, aimed to provide clinical decision making support for treatment of patients with Acute Coronary Syndrome.