Ivo Bussoloti Filho

Prevalence of human papillomavirus (HPV) in oral cavity and oropharynx

The prevalence of human papillomavirus (HPV) in the oral cavity and oropharynx has not yet been as well studied as its infection of the vaginal tract. However, new study are emerge after the development of molecular biology techniques. The objective of this study is to show the prevalence of HPV in the oral cavity and the oropharynx. An ample bibliographic review was done showing a prevalence of HPV 6, 11 in a normal oral mucous membrane (latent infection). In oral benign lesions associated with HPV, a prevalence of HPV 6 and 11 was observed in squamous cell papilloma (SCP) and condylomas acuminatum, while HPV 2 and 57 were more prevalent in verruca vulgaris lesions. As for focal epithelial hyperplasia (FEH) and oral cancer, especially squamous cell carcinoma (SCC), the prevalence was of HPV 13 and 32, and HPV 16, respectively. The last findings are, nonetheless, controversial. The last findings are, nonetheless, controversial. Showed also discrepancy result the prevalence of human papillomavirus (HPV) in normal oral mucous (latent infection) and in oral cancer, however evidenced confirmatory result in oral benign lesions associated with virus.

Prevalência do papilomavírus humano (HPV) na cavidade oral e na orofaringe

A prevalencia do papilomavirus humano (HPV) na cavidade oral e na orofaringe ainda nao esta bem esclarecida como nos estudos do trato genital, na qual e bem definida. Entretanto, novas pesquisas estao surgindo apos o aparecimento dos exames de biologia molecular. Neste estudo foi realizada uma revisao da literatura com o objetivo de verificar a prevalencia do papilomavirus humano na cavidade oral e na orofaringe. Os resultados desta pesquisa mostraram uma prevalencia do HPV 16 na mucosa oral normal (infeccao latente). Ja nas lesoes benignas orais associadas ao HPV mostraram uma prevalencia do HPV 6 e 11 em papilomas de celulas escamosas e condilomas, e, nas verrugas, uma prevalencia do HPV 2 e 57, enquanto na hiperplasia epitelial focal prevaleceram os HPVs 13 e 32, e no câncer oral, principalmente, no carcinoma de celulas escamosas (CCE), foi evidenciada uma alta prevalencia do HPV 16, o que sugere sua participacao na carcinogenese oral, apesar de ser um assunto controverso. Constatou-se tambem uma enorme discrepância nos resultados da prevalencia do papilomavirus humano (HPV) na mucosa oral normal (infeccao latente) e no câncer oral, enquanto nas lesoes benignas associadas ao virus, os resultados foram confirmatorios.

The frequency of human papillomavirus findings in normal oral mucosa of healthy people by PCR

UNLABELLED The human papillomavirus (HPV) is a DNA virus, which belongs to papillomaviridae family, being of low and high risk, which infect the skin and mucous membranes and can induce benign and malign tumor formation. In the oral mucosa they have been associated with oral papilloma, focal epithelial hyperplasia, leucoplakia and oral neoplasia. AIM to study the frequency of HPV finding in oral mucosa of normal people. MATERIALS AND METHODS Prospective study, cross-sectional cohort. One hundred volunteers, young adults, healthy, aged between 20 and 31 years, university students with no history, no complains, without oral or oropharyngeal lesions. They were submitted to a questionnaire with questions regarding HPV infection epidemiology. The samples were harvested by brushing and analyzed by PCR. RESULTS The results were negative for HPV in all samples. CONCLUSION It seems we had high social and economical class individuals, with nutrition rich in carotenoyds and vitamin C, low smoking and alcohol consumption and heterosexual habits with predominant monogamy and regular use of condoms.

HPV detection in the oral and genital mucosa of women with positive histopathological exam for genital HPV, by means of the PCR

UNLABELLED Infection by the Human Papilloma Virus (HPV) is one of the most frequent sexually transmitted diseases all over the world. The relationship between oral and genital HPV remains uncertain, as it is with its role on oral carcinogenesis. The goal of the present investigation was to check for the presence of HPV DNA in the oral and genital mucosas of women with HPV genital infection, using the polymerase chain reaction (PCR). STUDY METHOD Cross-sectional cohort. MATERIALS AND METHODS This is a pilot and prospective study involving 30 women, aged between 14 and 51 years, with HPV genital infection, confirmed by histopathology. All the patients were submitted to the exam and sample collection by swabbing the oral and genital mucosas in order to test for HPV DNA through the PCR technique. RESULTS None of the oral cavity samples were positive for HPV, while in the genital tract, HPV was detected in 17 (57%) of the 30 patients, especially HPVs 6b and 16. CONCLUSION Results show a higher percentage of genital HPV in comparison to the oral cavity, and suggest that genital HPV does not seem to be a predisposing factor for the oral infection in the same patient.

Detecção de HPV na mucosa oral e genital pela técnica PCR em mulheres com diagnóstico histopatológico positivo para HPV genital

Infection by the Human Papilloma Virus (HPV) is one of the most frequent sexually transmitted diseases all over the world. The relationship between oral and genital HPV remains uncertain, as it is with its role on oral carcinogenesis. The goal of the present investigation was to check for the presence of HPV DNA in the oral and genital mucosas of women with HPV genital infection, using the polymerase chain reaction (PCR). STUDY METHOD: Cross-sectional cohort. MATERIALS AND METHODS: this is a pilot and prospective study involving 30 women, aged between 14 and 51 years, with HPV genital infection, confirmed by histopathology. All the patients were submitted to the exam and sample collection by swabbing the oral and genital mucosas in order to test for HPV DNA through the PCR technique. RESULTS: none of the oral cavity samples were positive for HPV, while in the genital tract, HPV was detected in 17 (57%) of the 30 patients, especially HPVs 6b and 16. CONCLUSION: Results show a higher percentage of genital HPV in comparison to the oral cavity, and suggest that genital HPV does not seem to be a predisposing factor for the oral infection in the same patient.

Prevalence of human papillomavirus (HPV) DNA in oral mucosa of men with anogenital HPV infection

OBJECTIVE The aim of the present study was to determine the prevalence of concurrent oral and anogenital human papillomavirus (HPV) infection in male patients using polymerase chain reaction and reverse hybridization. STUDY DESIGN Thirty consecutive men were recruited among those attending the Clinic for Sexually Transmitted Diseases of the Outpatient Department of Dermatology and Sexually Transmitted Diseases, Santa Casa Hospital, São Paulo. The criteria for enrollment in the study were: age between 15 and 60 years, negative human immunodeficiency virus (HIV) status, and the presence of HPV anogenital lesion(s) confirmed by polymerase chain reaction and reverse hybridization. As a part of their management, all patients were subjected to punch biopsy of the anogenital lesions to confirm HPV and an HIV blood test to ensure that they were HIV negative. All patients had their oral mucosa examined with an artificial light. All oral lesions were sampled by biopsy, and subjects with no detectable lesions were sampled by oral mucosal scraping for HPV testing by polymerase chain reaction and reverse hybridization. All patients also completed a questionnaire that recorded their sexual preferences (heterosexual or homosexual, monogamous or polygamous), frequency of sexual activity, practice of oral and/or anal sex, and the use of condoms. RESULTS Only 3 patients presented with a clinically detectable oral lesion. Among them, just 1 was HPV positive by the molecular assay. In all of the other patients (27 out of 30), oral clinical lesions were not detected and scrapings were all negative for HPV infection. CONCLUSION The prevalence of concurrent oral and anogenital HPV infection was very low in this study (1 out of 30).

Evaluation of the concordance of sialometry and salivary glands scintigraphy in dry mouth patients

INTRODUCTION Many diagnostic tests are used to evaluate dry mouth patients, especially the ones with Sjögrens Syndrome, to whom these tests are part of classification criteria for scientific studies. AIM Thus, the concordance between results of sialometry and salivary glands scintigraphy was evaluated; if positive, it would enable the choice of one or the other for diagnosis. PATIENTS AND METHOD Seventy-two dry mouth patients were divided into non-Sjögrens Syndrome group, primary Sjögrens Syndrome group and secondary Sjögrens Syndrome group. The concordance among sialometry and scintigraphy results was evaluated by Kappa test. RESULTS It was observed that their concordance was equal or near to zero. CONCLUSION It is not possible to make a choice between these tests and both should be performed.

Prevalência de achados sugestivos de papilomavírus humano (HPV) em biópsias de carcinoma espinocelular de cavidade oral e orofaringe: estudo preliminar

Human papillomavirus (HPV) is considered to be an etiologic agent of cervical cancer and, recently its relation to oral and oropharyngeal cancer has also been investigated. Oral squamous cell carcinoma (SCC) represents 90% of all malignant tumors that affect the oral cavity. The prevalence of HPV in patients with SCC ranges from 0 to 100%. The most known viral cytopathic effect is koilocytosis, considered to be a major characteristic of HPV infection. AIM: The aim of this study was to verify the prevalence of some peculiar characteristics of HPV - koilocytosis - in oral and oropharyngeal SCC. STUDY DESIGN: transversal cohort. MATERIAL AND METHOD: Twenty slides with oral and/or oropharyngeal SCC were examined under microscopy. RESULTS: in 15 of them, koilocytosis was found, amounting to 75%. Although we know that polymerase chain reaction (PCR) is the method with the best sensitivity for HPV detection, we began this research looking for koilocytosis, which is highly suggestive of HPV infection. CONCLUSION: This study is a trial project and we will continue this research with PCR measures to confirm this high prevalence of HPV infection in oral and oropharyngeal SCC.

Prevalence of histological findings of human papillomavirus (HPV) in oral and oropharyngeal squamous cell carcinoma biopsies: preliminary study

UNLABELLED Human papillomavirus (HPV) is considered to be an etiologic agent of cervical cancer and, recently its relation to oral and oropharyngeal cancer has also been investigated. Oral squamous cell carcinoma (SCC) represents 90% of all malignant tumors that affect the oral cavity. The prevalence of HPV in patients with SCC ranges from 0 to 100%. The most known viral cytopathic effect is koilocytosis, considered to be a major characteristic of HPV infection. AIM The aim of this study was to verify the prevalence of some peculiar characteristics of HPV--koilocytosis--in oral and oropharyngeal SCC. STUDY DESIGN Transversal cohort. MATERIAL AND METHOD Twenty slides with oral and/or oropharyngeal SCC were examined under microscopy. RESULTS In 15 of them, koilocytosis was found, amounting to 75%. Although we know that polymerase chain reaction (PCR) is the method with the best sensitivity for HPV detection, we began this research looking for koilocytosis, which is highly suggestive of HPV infection. CONCLUSION This study is a trial project and we will continue this research with PCR measures to confirm this high prevalence of HPV infection in oral and oropharyngeal SCC.

Craniofacial pain and anatomical abnormalities of the nasal cavities.

The causal relation between anatomical variations of the nose and headaches and facial pain is analyzed through literature review of the topic. The pathogenesis that can be involved in this relation proves to be wider than simple alteration of nasal septum and turbinates that can cause mechanical stimulus through contact between these structures, which covers infectious factors, neurogenic inflammation, correlation with migraines and the role of nasal obstruction. The clinical findings of a lot of authors including the test with topical anesthetic to prove this causal relation, the indication of surgical treatment, in addition to good results of this treatment, are reported. The mechanism of pain relief obtained through surgical correction of nasal septum and turbinate is discussed. These data make us conclude that there are multiple etiologic factors involved, which makes us question the fundamental role of the mechanical aspect.