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Dive into the research topics where Iwona Gientka is active.

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Featured researches published by Iwona Gientka.


Applied Microbiology and Biotechnology | 2015

Accumulation and metabolism of selenium by yeast cells.

Marek Kieliszek; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel

This paper examines the process of selenium bioaccumulation and selenium metabolism in yeast cells. Yeast cells can bind elements in ionic from the environment and permanently integrate them into their cellular structure. Up to now, Saccharomyces cerevisiae, Candida utilis, and Yarrowia lipolytica yeasts have been used primarily in biotechnological studies to evaluate binding of minerals. Yeast cells are able to bind selenium in the form of both organic and inorganic compounds. The process of bioaccumulation of selenium by microorganisms occurs through two mechanisms: extracellular binding by ligands of membrane assembly and intracellular accumulation associated with the transport of ions across the cytoplasmic membrane into the cell interior. During intracellular metabolism of selenium, oxidation, reduction, methylation, and selenoprotein synthesis processes are involved, as exemplified by detoxification processes that allow yeasts to survive under culture conditions involving the elevated selenium concentrations which were observed. Selenium yeasts represent probably the best absorbed form of this element. In turn, in terms of wide application, the inclusion of yeast with accumulated selenium may aid in lessening selenium deficiency in a diet.


Applied Microbiology and Biotechnology | 2016

Rhodotorula glutinis—potential source of lipids, carotenoids, and enzymes for use in industries

Anna M. Kot; Stanisław Błażejak; Agnieszka Kurcz; Iwona Gientka; Marek Kieliszek

Rhodotorula glutinis is capable of synthesizing numerous valuable compounds with a wide industrial usage. Biomass of this yeast constitutes sources of microbiological oils, and the whole pool of fatty acids is dominated by oleic, linoleic, and palmitic acid. Due to its composition, the lipids may be useful as a source for the production of the so-called third-generation biodiesel. These yeasts are also capable of synthesizing carotenoids such as β-carotene, torulene, and torularhodin. Due to their health-promoting characteristics, carotenoids are commonly used in the cosmetic, pharmaceutical, and food industries. They are also used as additives in fodders for livestock, fish, and crustaceans. A significant characteristic of R. glutinis is its capability to produce numerous enzymes, in particular, phenylalanine ammonia lyase (PAL). This enzyme is used in the food industry in the production of l-phenylalanine that constitutes the substrate for the synthesis of aspartame—a sweetener commonly used in the food industry.


Molecules | 2014

Evaluation of the efficiency of different disruption methods on yeast cell wall preparation for β-glucan isolation.

Anna Bzducha-Wróbel; Stanisław Błażejak; Anna Kawarska; Lidia Stasiak-Różańska; Iwona Gientka; Ewa Majewska

Selected methods for yeast cell disruption were evaluated to establish their suitability for cell wall preparation in the process of β-glucan isolation. The effect of different disruption methods on contents of total saccharides, β-glucans and proteins in the produced cell walls preparations was analyzed. The degree of cell wall purification from intracellular components was established on the basis of the ratio of solubilised material. The investigated methods included: cell exposure to hot water (autoclaving), thermally-induced autolysis, homogenization in a bead mill, sonication and their combinations. Experimental systems were prepared in water (pH 5.0 and pH 7.0) and Tris-HCl buffer (pH 8.0). The Saccharomyces cerevisiae yeast cell wall preparations with the highest degree of cytosol component release and purification of β-glucans were produced by 30 min of cell homogenization with zirconium-glass beads (0.5 mm in diameter). This was confirmed by the highest ratio of solubilised material (approx. 64%–67%). The thus-produced preparations contained ca. 60% of total saccharides, 13%–14% of β(1,3)/(1,6)-glucans, and approx. 35% of crude proteins. Similar results were obtained after autolysis coupled with bead milling as well as with sonication, but the time required for these processes was more than 24 h. Homogenization in a bead mill could be valuable for general isolation procedures because allows one to eliminate the different autolytic activity of various yeast strains.


Microbial Cell Factories | 2018

Torulene and torularhodin: “new” fungal carotenoids for industry?

Anna M. Kot; Stanisław Błażejak; Iwona Gientka; Marek Kieliszek; Joanna Bryś

Torulene and torularhodin represent the group of carotenoids and are synthesized by yeasts and fungi. The most important producers of these two compounds include yeasts of Rhodotorula and Sporobolomyces genera. The first reports confirming the presence of torulene and torularhodin in the cells of microorganisms date to the 1930s and 1940s; however, only in the past few years, the number of works describing the properties of these compounds increased. These compounds have strong anti-oxidative and anti-microbial properties, and thus may be successfully used as food, feedstock, and cosmetics additives. In addition, tests performed on rats and mice showed that both torulene and torularhodin have anti-cancerous properties. In order to commercialize the production of these two carotenoids, it is necessary to obtain highly efficient yeast strains, for example, via mutagenization and optimization of cultivation conditions. Further studies on the activity of torulene and torularhodin on the human body are also needed.


BioMed Research International | 2017

Identification and Characterization of Oleaginous Yeast Isolated from Kefir and Its Ability to Accumulate Intracellular Fats in Deproteinated Potato Wastewater with Different Carbon Sources

Iwona Gientka; Marek Kieliszek; Karolina Jermacz; Stanisław Błażejak

The search for efficient oleaginous microorganisms, which can be an alternative to fossil fuels and biofuels obtained from oilseed crops, has been going on for many years. The suitability of microorganisms in this regard is determined by their ability to biosynthesize lipids with preferred fatty acid profile along with the concurrent utilization of energy-rich industrial waste. In this study, we isolated, characterized, and identified kefir yeast strains using molecular biology techniques. The yeast isolates identified were Candida inconspicua, Debaryomyces hansenii, Kluyveromyces marxianus, Kazachstania unispora, and Zygotorulaspora florentina. We showed that deproteinated potato wastewater, a starch processing industry waste, supplemented with various carbon sources, including lactose and glycerol, is a suitable medium for the growth of yeast, which allows an accumulation of over 20% of lipid substances in its cells. Fatty acid composition primarily depended on the yeast strain and the carbon source used, and, based on our results, most of the strains met the criteria required for the production of biodiesel. In particular, this concerns a significant share of saturated fatty acids, such as C16:0 and C18:0, and unsaturated fatty acids, such as C18:1 and C18:2. The highest efficiency in lipid biosynthesis exceeded 6.3 g L−1. Kazachstania unispora was able to accumulate the high amount of palmitoleic acid.


Polish Journal of Food and Nutrition Sciences | 2009

SHIKIMATE PATHWAY IN YEAST CELLS: ENzYMES, FUNCTIONING, REGULATION - A REVIEW

Iwona Gientka; Wanda Duszkiewicz-Reinhard


Electronic Journal of Biotechnology | 2017

Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis

Anna M. Kot; Stanisław Błażejak; Agnieszka Kurcz; Joanna Bryś; Iwona Gientka; Anna Bzducha-Wróbel; Magdalena Maliszewska; Lidia Reczek


Electronic Journal of Biotechnology | 2015

Biodegradation of deproteinized potato wastewater and glycerol during cultivation of Rhodotorula glutinis yeast

Anna Maria Kot; Stanisław Błażejak; Agnieszka Kurcz; Iwona Gientka


Fungal Biology Reviews | 2017

Biotechnological use of Candida yeasts in the food industry: A review

Marek Kieliszek; Anna M. Kot; Anna Bzducha-Wróbel; Stanisław BŁażejak; Iwona Gientka; Agnieszka Kurcz


European Food Research and Technology | 2014

Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka

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Stanisław Błażejak

Warsaw University of Life Sciences

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Anna Bzducha-Wróbel

Warsaw University of Life Sciences

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Lidia Stasiak-Różańska

Warsaw University of Life Sciences

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Marek Kieliszek

Warsaw University of Life Sciences

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Agnieszka Kurcz

Warsaw University of Life Sciences

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Anna M. Kot

Warsaw University of Life Sciences

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Anna Maria Kot

Warsaw University of Life Sciences

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Edyta Lipińska

Warsaw University of Life Sciences

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Joanna Bryś

Warsaw University of Life Sciences

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