J.B. van Lier

Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays

The application of anaerobic digestion technology is growing worldwide because of its economic and environmental benefits. As a consequence, a number of studies and research activities dealing with the determination of the biogas potential of solid organic substrates have been carrying out in the recent years. Therefore, it is of particular importance to define a protocol for the determination of the ultimate methane potential for a given solid substrates. In fact, this parameter determines, to a certain extent, both design and economic details of a biogas plant. Furthermore, the definition of common units to be used in anaerobic assays is increasingly requested from the scientific and engineering community. This paper presents some guidelines for biomethane potential assays prepared by the Task Group for the Anaerobic Biodegradation, Activity and Inhibition Assays of the Anaerobic Digestion Specialist Group of the International Water Association. This is the first step for the definition of a standard protocol.

A review: The anaerobic treatment of sewage in UASB and EGSB reactors

The anaerobic treatment process is increasingly recognized as the core method of an advanced technology for environmental protection and resource preservation and it represents, combined with other proper methods, a sustainable and appropriate wastewater treatment system for developing countries. Anaerobic treatment of sewage is increasingly attracting the attention of sanitary engineers and decision makers. It is being used successfully in tropical countries, and there are some encouraging results from subtropical and temperate regions. In this review paper, the main characteristics of anaerobic sewage treatment are summarized, with special emphasis on the upflow anaerobic sludge blanket (UASB) reactor. The application of the UASB process to the direct treatment of sewage is reviewed, with examples from Europe, Asia and the Americas. The UASB reactor appears today as a robust technology and is by far the most widely used high-rate anaerobic process for sewage treatment.

Sodium inhibition of acetoclastic methanogens in granular sludge from a UASB reactor

Abstract The effect of sodium on the formation of methane from acetate in granular sludge from a UASB reactor has been determined at various acetate concentrations and pH-levels. At neutral pH sodium concentrations of 5, 10 and 14 g Na+l−1caused 10, 50 and 100% inhibition respectively, relative to the maximum specific acetoclastic methanogenic activity of the granular sludge. These values reflect the sensitivity of Methanothrix sp. towards sodium, as this is the predominant acetoclastic methanogen in the granular sludge used in this study. The pH did not affect the inhibitory action of sodium significantly in the range 6.5–7.2, but at pH-levels near 8 the inhibition was more pronounced. At acetate concentrations below 500 mg l−1diffusion limitation partly masked the influence of sodium on the specific activity of the granular sludge. No adaptation of Methanothrix sp. to high sodium concentrations could be obtained in a period of 12 weeks. Net growth of Methanothrix sp. could be obtained at sodium concentrations of approx. 10 g l−1. At equal sodium concentrations, sodium chloride had a somewhat stronger effect on the specific activity than sodium sulfate, but the difference is unimportant for design purposes. The results obtained with short term activity measurements can reliably be used for predictions of the effect of sodium salts on continuously fed UASB reactors.

Advanced anaerobic wastewater treatment in the near future

New insights into the anaerobic degradation of very different categories of compounds, and into process and reactor technology will lead to very promising new generations of anaerobic treatment system, such as ‘Expanded Granular Sludge Bed’ (EGSB) and ‘Staged Multi-Phase Anaerobic’ (MPSA) reactor systems. These concepts will provide a higher efficiency at higher loading rates, are applicable for extreme environmental conditions (e.g. low and high temperatures) and to inhibitory compounds. Moreover, by integrating the anaerobic process with other biological methods (sulphate reduction, micro-aerophilic organisms) and with physical-chemical methods, a complete treatment of the wastewater can be accomplished at very low costs, while at the same time valuable components can be recovered for reuse.

Effects of thermo-chemical pre-treatment on anaerobic biodegradability and hydrolysis of lignocellulosic biomass.

The effects of different thermo-chemical pre-treatment methods were determined on the biodegradability and hydrolysis rate of lignocellulosic biomass. Three plant species, hay, straw and bracken were thermo-chemically pre-treated with calcium hydroxide, ammonium carbonate and maleic acid. After pre-treatment, the plant material was anaerobically digested in batch bottles under mesophilic conditions for 40 days. From the pre-treatment and subsequent anaerobic digestion experiments, it was concluded that when the lignin content of the plant material is high, thermo-chemical pre-treatments have a positive effect on the biodegradability of the substrate. Calcium hydroxide pre-treatment improves the biodegradability of lignocellulosic biomass, especially for high lignin content substrates, like bracken. Maleic acid generates the highest percentage of dissolved COD during pre-treatment. Ammonium pre-treatment only showed a clear effect on biodegradability for straw.

Sequential anaerobic–aerobic treatment for domestic wastewater – A review

Introduction, consolidation and even standardization of expensive conventional aerobic systems for domestic wastewater treatment imposed significant financial constraints on the expansion of sanitary services including treatment in developing countries. A viable alternative is the sequential anaerobic-aerobic systems. If compared with the conventional aerobic technologies based on activated sludge processes, lower energy consumption and lower excess sludge production can be achieved with a high-rate anaerobic pre-treatment step. Particularly with concentrated sewage, the energy benefit of applying anaerobic pre-treatment will become very significant. This study aims on putting the effectiveness of sequential systems for treatment of domestic wastewater on view, through displaying results presented in literature on the performance of these systems.

Temperature effects on the oxygen transfer rate between 20 and 55°C

Abstract The influence of temperature on the oxygen transfer rate (OTR) was studied in a bubble column. Aeration took place in three different liquids: tapwater, anaerobically pretreated paper process water and thermophilic sludge grown on a mineral medium and volatile fatty acids as carbon source. The OTR was measured in a temperature range of 20–55°C in case of tap- and process water. The OTR in the thermophilic sludge was determined at 55°C.The OTR remained constant over the specified temperature range in case of tapwater and showed a slight increase in case of process water. The constant OTR in case of tapwater was due to the counteracting effect of an increased overall oxygen transfer coefficient versus the decreased oxygen saturation concentration at higher temperatures. At 55°C the OTR in the thermophilic sludge was comparable to both other liquids at this temperature.

Azo dye reduction by thermophilic anaerobic granular sludge, and the impact of the redox mediator anthraquinone-2,6-disulfonate (AQDS) on the reductive biochemical transformation

Azo dye reduction at 55°C by thermophilic anaerobic granular sludge was investigated distinguishing between the biotic and abiotic mechanisms. The impact of the redox mediator anthraquinone-2,6-disulfonate (AQDS) on colour removal and co-substrate oxidation was also investigated. Metabolic activities of the thermophilic inoculum induced a fast azo dye reduction and indicated a biotic predominance in the process. The addition of co-substrate enhanced the decolourisation rates 1.7-fold compared with the bottles free of co-substrate. Addition of AQDS together with co-substrate enhanced the k value 1.5-fold, compared with the incubation containing co-substrate in the absence of AQDS. During a comparative study between sludge samples incubated under mesophilic (30°C) and thermophilic (55°C) conditions, the decolourisation rate at 55°C reached values up to sixfold higher than at 30°C. Biological treatment at 55°C showed a fast initial generation of reducing compounds via co-substrate oxidation, with AQDS increasing the azo dye reduction rate in all the incubations tested. Nevertheless, high concentrations of AQDS showed severe inhibition of thermophilic acetate and propionate oxidation and methane production rates. These promising results indicate that there may be good prospects for thermophilic anaerobic treatment of other reductive transformations such as reduction of nitroaromatics and dehalogenation.

Mesophilic and thermophilic activated sludge post-treatment of paper mill process water.

Increasing system closure in paper mills and higher process water temperatures make the applicability of thermophilic treatment systems increasingly important. The use of activated sludge as a suitable thermophilic post-treatment system for anaerobically pre-treated paper process water from a paper mill using recycled wastepaper was studied. Two lab-scale plug flow activated sludge reactors were run in parallel for 6 months; a thermophilic reactor at 55 degrees C and a reference reactor at 30 degrees C. Both reactors were operated simultaneously at 20, 15 and 10 days SRT. The effects of temperature and SRT on sludge settleability and chemical oxygen demand (COD) removal efficiencies of different fractions were studied. Total COD removal percentages over the whole experimental period were 58+/-5% at 30 degrees C and 48 +/- 10% at 55 degrees C. The effect of the SRT on the total COD removal was negligible. Differences in total COD removal between both systems were due to a lesser removal of soluble and colloidal COD at 55 degrees C compared to the reference system. At 30 degrees C, colloidal COD removal percentages were 65+/-25%, 75+/-17% and 86+/-22% at 20, 15 and 10 days SRT, respectively. At 55 degrees C, these percentages were 48+/-34%, 40+/-28% and 70+/-25%, respectively. The effluent concentrations of colloidal COD in both systems were related to the influent concentration of colloidal material. The thermophilic sludge was not able to retain influent colloidal material as well as the mesophilic sludge causing a higher thermophilic effluent turbidity. Sludge settling properties were excellent in both reactor systems. These were neither temperature nor SRT dependent but were rather caused by extensive calcium precipitation in the aeration tanks creating a very dense sludge. For application in the board industry, a thermophilic in line treatment system seems feasible. The higher effluent turbidity is most likely offset by the energy gains of treatment under thermophilic conditions.

Impact of inflow conditions on activated sludge filterability and membrane bioreactor (MBR) operational performance

Operation and performance of membrane bioreactors (MBRs) for wastewater treatment is influenced by changes in activated sludge properties. Therefore, understanding of the factors influencing sludge filterability is of major importance for efficient operation of MBR installations. This paper assesses the impact of the influent characteristics on activated sludge filterability and treatment performance of full-scale MBRs. The full-scale MBRs were closely monitored both in summer and winter. The measurement campaign, consisting of activated sludge filterability tests and physicochemical analyses, is supplemented with operational performance data evaluation of the various full-scale plants. Based on these data, it is shown that an undesired and refractory composition of incoming wastewater, hydraulic and/or organic load shocks, as well as abrupt temperature changes of the influent, lead to operational problems and affect sludge filterability.