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Dive into the research topics where J. Badenhorst is active.

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Featured researches published by J. Badenhorst.


International Journal of Environmental Science and Technology | 2011

Effect of nitrogen loading rates on nitrogen removal by using a biological filter proposed for ventilated improved pit latrines

M. Coetzee; M Van der Merwe; J. Badenhorst

Pit latrines are the most frequently used sanitation systems in developing countries because of weak infrastructure and poor economic wealth. A modified ventilated improved pit latrine, with a biological filter beneath is proposed to stabilize and to remove the bulk of the nitrogen from the liquid phase. Although the hydraulic loading rate in the proposed biological filter system was calculated to be ca 36 L/m2/d, significantly lower than the rates that are typical applied in standard rate biological filters (in the range of 1000–4000 L/m2/d) used to treat domestic wastewater; the total Kjeldahl nitrogen and chemical oxygen demand concentrations are significantly higher in faecal sludge, namely 3–5 g /L and 20–50 g /L, respectively compared to ca 60 mg/L and 500 mg/L in standard rate biological filters. The biological filter was operated at nitrogen loading rates of 72, 145, 290 and 435 g/m2/d, respectively, until stable state conditions were obtained. The biological filter showed effective nitrogen removal between 72 and 434 g/m2/d and the best total nitrogen removal was obtained at 145 g/m2/d, namely 92 %. These results suggest that it should be possible to remove nitrogen effectively using a biological filter beneath a modified ventilated improved pit latrine.


Journal of Applied Microbiology | 2017

Hydrolysis of nitriles by soil bacteria: Variation with soil origin

Okl Rapheeha; M.P. Roux-van der Merwe; J. Badenhorst; Varsha Chhiba; Moira L. Bode; Kgama Mathiba; Dean Brady

The aim of this study was to explore bacterial soil diversity for nitrile biocatalysts, in particular, those for hydrolysis of β‐substituted nitriles, to the corresponding carboxamides and acids that may be incorporated into peptidomimetics. To achieve this, we needed to compare the efficiency of isolation methods and determine the influence of land use and geographical origin of the soil sample.


African Zoology | 2016

DNA barcoding for identification of cryptic species in the field and existing museum collections: a case study of Aethomys and Micaelamys (Rodentia: Muridae)

Metlholo Andries Phukuntsi; Helene Brettschneider; Desiré L. Dalton; Teresa Kearney; J. Badenhorst; Antoinette Kotze

DNA barcoding has been proposed as a method for species identification. However, this method has been criticised for its over-reliance on a single mitochondrial gene. In this study, four mitochondrial gene regions and one nuclear gene region were used to investigate their different abilities to identify tissue associated with museum specimens of Aethomys chrysophilus, Aethomys ineptus and Micaelamys namaquensis. Aethomys chrysophilus and the more recently elevated A. ineptus are indistinguishable on morphological grounds; however, their ranges are largely parapatric with only one syntopic locality currently known. All of the mitochondrial gene regions were able to separate M. namaquensis from A. chrysophilus and A. ineptus, but they varied in their abilities to resolve differences between A. chrysophilus and A. ineptus. The sequence results identified a specimen from KwaZulu-Natal that was misclassified and should have been identified as A. ineptus. Seven specimens that had not been reclassified following the elevation of A. ineptus to species level were identified as A. ineptus. Individuals of A. chrysophilus from Malawi could not be classified as either A. chrysophilus or A. ineptus, and may be a hybrid or a new, distinct species. This study indicates that DNA barcoding may be used to separate M. namaquensis from A. chrysophilus and A. ineptus, and although it was not able to separate A. chrysophilus and A. ineptus, it did indicate specimens from Malawi may be a new cryptic species.


Journal of Phylogenetics & Evolutionary Biology | 2016

Hidden Identities: Cryptic Species in the Otomys Genus (Cuvier 1824) (Rodentia: Muridae: Otomyinae) Revealed by Mitochondrial and Nuclear DNA in South Africa

Metlholo Andries Phukuntsi; Teresa Kearney; Helene Brettschneider; Desiré L. Dalton; Maria Oosthuizen; Gerhard Goldner; J. Badenhorst; Antoinette Kotze

The recent taxonomy and phylogeny of Otomyinae has been in a state of flux as new systematic revisions combining molecular, karyotypic and morphometric information have identified changes at various taxonomic levels. Currently two genera of Otomyinae and eight species of Otomys are recognized in South Africa. However, the position of Otomys sloggetti on the phylogeny of Otomyinae has not been resolved, and since this species was not well represented in recent revisions it may also reveal multiple cryptic evolutionary species. In this study four mitochondrial and one nuclear gene regions and external morphological characters were analysed to elucidate relationships within O. sloggetti, as well as between O. sloggetti and other Otomys species occurring in South Africa. The data from this study suggested O. sloggetti belongs to neither the Otomys, nor the Parotomys genera. Instead, we propose returning to the classification of Otomyinae and recognize Myotomys as the valid genus for O. sloggetti. Within O. sloggetti, our data does not support the traditional view of the distribution and intraspecific variation of the species, and invites a new hypothesis. Specimens identified in the field as O. sloggetti were found to represent two different clades. One of the clades was genetically and morphologically consistent with the description for O. sloggetti, while the other was distinct from O. sloggetti and other Otomys species known to occur in South Africa. Our data suggests that this is a novel species within the Otomys genus.


Current Microbiology | 2013

Characterisation of Two Bifunctional Cellulase–Xylanase Enzymes Isolated from a Bovine Rumen Metagenome Library

K. J. Rashamuse; Daniel F. Visser; Fritha Hennessy; J. Kemp; M.P. Roux-van der Merwe; J. Badenhorst; T. Ronneburg; R. Francis-Pope; Dean Brady


World Journal of Microbiology & Biotechnology | 2005

Fungal Treatment of an Edible-oil-containing Industrial Effluent

M. P. Roux Van der Merwe; J. Badenhorst; Trevor J. Britz


Applied Clay Science | 2013

Microwave assisted green synthesis and characterization of silver/montmorillonite heterostructures with improved antimicrobial properties

S. Kesavan Pillai; S. Sinha Ray; Manfred Scriba; Jayita Bandyopadhyay; M.P. Roux-van der Merwe; J. Badenhorst


Industrial Crops and Products | 2015

Control of Fusarium spp. causing damping-off of pine seedlings by means of selected essential oils

L. Seseni; T. Regnier; M.P. Roux-van der Merwe; E. Mogale; J. Badenhorst


Water SA | 2011

The effect of air supply on nitrogen removal using a biological filter proposed for ventilated pit latrines

Martha A.A. Coetzee; Magaretha P. Roux-Van der Merwe; J. Badenhorst


Journal of Applied Phycology | 2018

Evaluating the usability of 19 effluents for heterotrophic cultivation of microalgal consortia as biodiesel feedstock

E. Jordaan; M.P. Roux-van der Merwe; J. Badenhorst; G. Knothe; B. M. Botha

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M.P. Roux-van der Merwe

Tshwane University of Technology

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Dean Brady

University of the Witwatersrand

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Antoinette Kotze

University of the Free State

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Desiré L. Dalton

University of the Free State

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Metlholo Andries Phukuntsi

Tshwane University of Technology

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Moira L. Bode

University of the Witwatersrand

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Teresa Kearney

National Museum of Natural History

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Kgama Mathiba

Council of Scientific and Industrial Research

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Varsha Chhiba

Council of Scientific and Industrial Research

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