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Dive into the research topics where J.E. Lillywhite is active.

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Featured researches published by J.E. Lillywhite.


Transactions of The Royal Society of Tropical Medicine and Hygiene | 1993

Immunodiagnosis of Strongyloides stercoralis infection: a method for increasing the specificity of the indirect Elisa

David J. Conway; Neil S Atkins; J.E. Lillywhite; J. W Bailey; Ralph D Robinson; John F Lindo; Donald A. P Bundy; Albert E Bianco

Indirect enzyme-linked immunosorbent assay (ELISA) allows sensitive detection of serum immunoglobulin (Ig) G against a soluble extract of Strongyloides stercoralis infective larvae. In this study, 40/40 (100%) human strongyloidiasis sera had high levels of anti-S. stercoralis IgG, but 30/40 (75%) filariasis sera, and 12/40 (30%) necatoriasis sera also had higher levels than control sera from UK residents. In attempts to increase the assay specificity by absorption of cross-reactive IgG, the effectiveness of pre-incubation of sera with extracts of different parasitic nematodes was investigated. One hour of incubation with 20 micrograms/ml aqueous extract of Onchocerca gutturosa absorbed cross-reactive IgG in most filariasis and necatoriasis sera, reducing the proportion with IgG levels above the positivity threshold by more than one-half. Preliminary results suggest that absorption with extracts of other filarial nematodes is equally effective, and that some of the cross-reactive IgG is directed against phosphorylcholine. Cross-reactive IgG in most necatoriasis sera was effectively absorbed with 20 micrograms/ml extract of Necator americanus. Cross-reactive IgG was not effectively absorbed with an extract of Ascaris lumbricoides. Absorption of cross-reactive IgG is an effective means of increasing the specificity of the indirect ELISA, for use in the immunodiagnosis and immuno-epidemiology of S. stercoralis infection.


Parasite Immunology | 1991

Humoral immune responses in human infection with the whipworm Trichuris trichiura

J.E. Lillywhite; D. A. P. Bundy; J.M. Didier; Ed Cooper; Albert E Bianco

Summary The humoral immune response to infection with Trichuris trichiura was investigated by ELISA and immunoblotting using human sera from the Caribbean island of St Lucia. Immunoblot analysis of the degree of cross‐reactivity with the related trichuroid Trichinella spiralis and with the other commonly co‐existent nematodes. Ascaris lumbricoides and Toxocara canis, was carried out using selected sera. The IgM, IgA, IgE, and IgG subclass antibody levels were measured in ELISA using a detergent solubilized extract of adult T. trichiura. The IgG and IgE responses were highly Trichuris specific. Anti‐T. trichiura IgM responses were totally cross‐reactive with A. lumbricoides and were completely ablated by pre‐incubation of sera with Ascaris antigen. The IgG response was predominantly of the IgG1 subclass with a minimal IgG3 response. Only 1 person out of 130 tested had a delectable IgG3 response. The IgG2 response appeared to be directed primarily against carbohydrate or polysaccharide antigens as pre‐treatment of the ELISA plates with poly‐L‐lysine was necessary before a response could be detected. These data are the first demonstration of human isotypic responses to infection with T. trichiura.


Parasitology | 1992

The relationship between Trichuris trichiura transmission intensity and the age-profiles of parasite-specific antibody isotypes in two endemic communities

C. S. Needham; D. A. P. Bundy; J.E. Lillywhite; J. M. Didier; I. Simmons; Albert E Bianco

The present study compares parasite-specific antibody responses in two Caribbean communities with high and low levels of Trichuris trichiura transmission. The age-dependency of antibody levels suggest that IgG1 and IgG2 levels relate to the current intensity of infection (as assessed by density of eggs in stool (e.p.g.) and reflect the age-intensity profile at the population level. IgG4, IgE and IgA levels persist into early adulthood and the subsequent decline is gradual. In the low transmission area, lower infection levels are reflected in lower parasite-specific antibody levels (of all isotypes) in the community as a whole. Despite a significantly greater past experience of infection in the high transmission area, antibody levels are not maintained at significantly higher levels throughout adulthood. The production of IgA appears to require a threshold for triggering, and a vigorous IgA response is maintained into early adulthood only in the high transmission village where peak intensity is greatest and the age-convexity of intensity is most marked. Experimental and theoretical studies focusing on the dynamic nature of host-helminth interactions in hosts exposed to high and low infection levels, and the putative role of acquired immunity, are discussed in relation to the data presented.


Parasite Immunology | 1991

Age-dependency of infection status and serum antibody levels in human whipworm (Trichuris trichiura) infection

Donald A. P Bundy; J.E. Lillywhite; J.M. Didier; I. Simmons; Albert E Bianco

Summary This study examines the age‐dependency of the relationships between human infection with whipworm (Trichuris trichiura) and parasite‐specific antibody level measured by ELISA against an extract of adult worms after preincubation of the sera with Ascaris lumbricoides adult worm extract. The convex age‐profile of parasite infection intensity is shown to be mirrored ban ase‐dependent change in age‐class mean levels of IgG (all subclasses except IgG3). IgA. IgM and IgE. Mean antibody levels rise with increasing acquisition of infection in childhood and decline as the intensity of infection falls in adulthood. Immunobiot analysis of selected sera from different age‐classes indicates that antigen recognition is simitath dependent on infection intensity. In individual children, antibody levels correlate positively with acquisition of infection, consistent with a simple model of antigen dosage specifying the magnitude of the humoral immune response. In adults, Igd correlates positively and IgA negatively with intensity of infection, suggesting involvement of these isolypes in functional roles of immune blockade or effector mechanisms, respectively.


Transactions of The Royal Society of Tropical Medicine and Hygiene | 1994

2. Immunological correlates with patterns of Trichuris infection

C. S. Needham; J.E. Lillywhite

The significance of parasite-specific serum and secretory immunoglobulin (Ig) isotype responses as determinants of Trichuris trichiura infection intensity in endemic communities is discussed. Comparison of age-dependent isotype responses and the age profiles of infection intensity in 2 endemic communities with markedly different levels of T. trichiura transmission suggest that serum IgA responses may reflect the accumulated past experience of infection and thus may be relevant in acquired immunity to T. trichiura and contribute to the age-convexity of infection intensity in areas of intense transmission. Preliminary analysis of data from a second community-based study shows that parasite-specific secretory IgA in saliva increases with age and correlates negatively with infection intensity, suggesting that secretory IgA may also be implicated in acquired immunity to this gut-dwelling nematode.


Parasite Immunology | 1995

Identification and characterization of excreted/secreted products of Trichuris trichiura

J.E. Lillywhite; Edward S Cooper; C. S. Needham; S. Venugopal; D. A. P. Bundy; Albert E. Bianco

This study provides the first description of the range and immunogenicity of proteins excreted and/or secreted by living T. trichiura adult worms following their recovery from the human large intestine. Metabolic labelling ofT. trichiura excretory/secretory (ES) products with [35S]‐methionine revealed a range of proteins with prominent components at 52–54 kDa, 35–45 kDa & 17 kDa. In contrast, the major component of unlabelled T. trichiura ES, somatic whole worm and isolated stichosome extracts, and of [35S]‐methionine labelled somatic extracts, was present at approximately 47 kDa. Similarly, the major 43 kDa protein present in unlabelled T. muris ES, somatic worm extract and [35S]‐methionine labelled somatic worm extract, was only weakly detected in labelled T. muris ES. Pulse chase experiments demonstrated that after 20 h, the 43 kDa was a prominent component of T. muris ES. These data suggest that the 43/47 kDa protein of Trichuris adult worms is not a major constituent of newly synthesized ES but is either synthesized at a slower rate than other proteins, or sequestered or stored, most likely in the stichocytes, before release. Immunoprecipitations using a range of sera from T. trichiura‐infec ted individuals demonstrated that many of the ES components are immunogenic. Antibody responses were vigorous in children with intense infections and negligible in parasitologically negative children. There was marked heterogeneity in responses to a 17 kDa antigen, with the age profile of anti‐17 kDa antibody levels reflecting age‐dependent infection intensities at the population level.


Parasite Immunology | 1993

Age-dependency of serum isotype responses and antigen recognition in human whipworm (Trichuris trichiura) infection

C. S. Needham; J.E. Lillywhite; J.M. Didier; Albert E. Bianco; D. A. P. Bundy

The present study examines the age‐dependency of parasite‐specific isotype responses and antigen recognition profiles of individuals within a Trichuris trichiura endemic community, in order to evaluate the significance of serum antibodies as determinants of observed age‐related patterns of infection intensity. A high degree of individual heterogeneity is observed in isotype responses to separated T. trichiura antigens by Western blot. Recognition by IgG1 antibodies exhibits marked age‐dependency. The age‐profiles of IgG1 responses to selected antigens of 16–17 kDa and 90 kDa molecular weight reflect the age‐related changes in current infection intensity at the population level. Similarly, mean age patterns of IgG2 responses to a 90 kDa antigen, and mean IgG4 responses to a 16–17 kDa antigen reflect mean infection levels. IgG3 responses are negligible, and for methodological reasons, both IgE and IgM specificities are not presented. IgA responses to separated antigens of 16–17 kDa and 90 kDa, exhibit age‐profiles which may suggest the development of an IgA‐mediated acquired resistance to T. trichiura with age. IgA levels remain elevated throughout early adulthood, when infection intensity levels markedly decrease, supporting the hypothesis that IgA antibodies may be significant in generating the convex nature of the age‐infection profile of T. trichiura.


Transactions of The Royal Society of Tropical Medicine and Hygiene | 1990

Dissociation of antibody responses during human schistosomiasis and evidence for enhancement of granuloma size by anti-carbohydrate IgM

Andrew J.G. Simpson; Xinyuan Yi; J.E. Lillywhite; Pirlanta Omer Ali; Charles Kelly; Richard Mott; S.Ronald Smithers; Rodrigo Corrêa Oliveira; Naftale Katz; Franklin David Rumjanek; Paul Hagan

Immunoglobulin (Ig) G and IgM antibody levels to soluble egg antigens (SEA), adult worm glycoproteins (AWGP), carbohydrate antigens (CHO) and cationic exchange fraction 6 (CEF6) were measured in serum specimens taken from Brazilian patients with acute, intestinal, hepato-intestinal and hepatosplenic schistosomiasis mansoni. The antibody levels varied among the groups, with the highest anti-egg antigen responses in the acute patients and the highest anti-adult worm responses in patients with chronic disease. The responses to the component parts of the egg antigens were dissociated, with anti-carbohydrate IgG and IgM responses being highest in the acute infection group and anti-CEF6 IgG responses being uniform among the clinical groups. The possibility of a direct role for anti-CHO antibody responses in egg-induced pathology was investigated using the mouse lung model. The anti-carbohydrate monoclonal antibody NIMP/M45 significantly enhanced granuloma formation. Mice given NIMP/M45 produced granulomas larger than those of naive mice or mice given an unrelated monoclonal antibody, and as large as those produced by mice which had been presensitized to egg antigens. The independent regulation of responses to egg antigens may indicate that such responses are minimized to reduce the pathological consequences of infection whilst allowing the development of protective anti-worm responses.


Parasitology | 1994

Temporal changes in Trichuris trichiura infection intensity and serum isotype responses in children

C. S. Needham; J.E. Lillywhite; J. M. Didier; Albert E. Bianco; D. A. P. Bundy

The present study describes Trichuris trichiura infections in a cohort of children over a period of approximately 5 years, and examines the relationships between changes in infection intensity and changes in parasite-specific isotype responses (measured by ELISA). The decrease in mean infection intensity with time was mirrored by time-dependent changes in IgG subclass responses, with IgA and IgE levels remaining relatively constant in the cohort. At the individual level, changes in infection intensity between the initial and final time-points correlated positively and significantly with changes in all isotype levels with the exception of IgA.


Acta Tropica | 1994

Comparison of age-dependent antigen recognition in two communities with high and low Trichuris trichiura transmission

Catherine S. Needham; J.E. Lillywhite; Joan M. Didier; A.Ted Bianco; Donald A. P Bundy

A previous ELISA-based study using whole worm extract, compared age-antibody profiles in two communities with high and low levels of Trichuris trichiura transmission (Needham et al., 1992). This showed that specific IgG1 levels mirrored infection intensity at the population level, while IgA levels exhibited a weak trend to remain elevated in the adult age classes in the area of highest transmission. This was interpreted as preliminary evidence for IgA-mediated resistance in the population with greatest prior experience of infection. The present study extends this work to compare IgG1 and IgA isotype recognition of separated antigens by Western blot between the two communities. Comparison of age-dependent antigen recognition in the two communities shows that both qualitative and quantitative recognition by IgG1 antibodies is related to the current intensity of infection (as assessed by eggs per gram of faeces, epg). The magnitude of the IgA response to separated antigens of 16-17 kDa and 90 kDa exhibits a stronger trend to remain elevated in adults and to reflect the past experience of infection: IgA antibodies are present at significantly higher levels in adults from the high transmission area compared with those from the community with low levels of T. trichiura endemicity, despite infection levels in these age groups being of similar magnitude. This comparative study therefore, provides further evidence to support a role for IgA in acquired immunity to T. trichiura in areas of intense transmission.

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Albert E. Bianco

Liverpool School of Tropical Medicine

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J.M. Didier

Imperial College London

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A.Ted Bianco

Liverpool School of Tropical Medicine

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Ed Cooper

Imperial College London

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