J. F. B. Morrison

Two groups of spinal interneurones that respond to stimulation of the abdominal viscera of the cat

1. Recordings have been made from sixty‐three spinal interneurones that received inputs from the abdominal viscera. These were divided into two groups: group A were sacral interneurones without either significant ascending projections or afferent input from the lumbar splanchnic nerves and group B were neurones with short ascending axons and afferent inputs from the lumbar splanchnic nerves, and often also from the pelvic and pudendal nerves.

An electrophysiological study of somatic and visceral convergence in the reflex control of the external sphincters

1. Mass wave and single unit discharges have been recorded from pudendal efferents innervating the external anal and urethral sphincters in chloralose anaesthetized or decerebrate cats.

Inhibitory interactions between colonic and vesical afferents in the micturition reflex of the cat.

1. In anaesthetized cats in which the only intact autonomic pathways innervating the colon and bladder were in the pelvis nerves, distension of the colon resulted in a graded inhibition of spontaneous bladder motility, and a decrease in the reflexly evoked waves of activity in the vesical branches of the pelvic nerves. 2. Electrical stimulation with voltages sufficient to excite only the myelinated fibres in the colonic branches of the pelvic nerve caused inhibition of spontaneous bladder motility, an increase in micturition threshold, and a reduction in the reflexly evoked waves of activity in the vesical efferents of the pelvic nerve. 3. The results demonstrate that the pelvic nerve afferent inflow from the colon produces a marked central inhibitory influence on the micturition reflex.

Factors that determine the excitability of parasympathetic reflexes to the cat bladder

1. Spino‐bulbo‐spinal reflex responses could be recorded from the vesical branches of the pelvic nerve following electrical stimulation of afferents in the vesical or colonic branches of the pelvic nerves, the hypogastric or pudendal nerves. The latencies of responses from these different sources were similar.

The influence of afferent inputs from skin and viscera on the activity of the bladder and the skeletal muscle surrounding the urethra in the rat.

(1) Somato-visceral and viscero-visceral reflex interactions have been studied in the bladder branches of the pelvic nerve and in the electromyographic (EMG) activity of the periurethral skeletal muscles of the anesthetized rat, and by observations of changes in bladder motility. (2) Slow distensions of the bladder caused some elevation of intravesical pressure, and culminated in a micturition contraction. Periurethral EMG activity increased gradually during the bladder distension, and showed an oscillatory marked increase during the bladder contraction. There was a small increase in pelvic nerve efferent activity during slow distension, and there was a substantial increase before, or at the start, of a micturition contraction. (3) Oscillatory bursting activity occurred in recordings of the EMG activity from periurethral skeletal muscle during the rising phase of micturition contraction; this was particularly so during the most rapid rise in intravesical pressure, and periods of electrical silence lasting 80-270 ms alternated with bursts of activity in the periurethral EMG. (4) In the present experiments, the switching mechanism activated by pelvic afferent signals related to intravesical pressure reversed the behavior of a number of reflex pathways. When the bladder pressure was low, nociceptive pinching of the perineal skin usually caused bladder contraction and a rise in pelvic nerve efferent activity and in periurethral EMG activity. When the bladder was full, micturition contractions were present and reduced in size and frequency by pinching of the perineal skin. The pelvic nerve efferent activity was correspondingly reduced, while the EMG activity increased during and following the nociceptive stimulus. Cooling the scrotal skin with ice also decreased the frequency of bladder contractions. (5) When the bladder pressure was low, distension of the anus and colon increased periurethral EMG activity, but did not affect bladder tone. However, when the bladder was full, these stimuli reduced the size and frequency of bladder contractions, associated with a reduction in the pelvic nerve efferent activity. There was usually a simultaneous reduction in the EMG activity in periurethral muscles. Similar results were obtained during distension of the seminal vesicles or vagina, or following injection of 20-60 microliters of saline into the lumen of the vas deferens. Reversal of the responses at extremes of intravesical pressure was observed in every case. (6) Following spinal transection at the upper cervical or thoracic level, micturition contractions were absent at high bladder volumes. However the effects described when the neuraxis was intact and the bladder pressure was low were still observed.(ABSTRACT TRUNCATED AT 400 WORDS)

Sensory and autonomic nerve changes in the monosodium glutamate‐treated rat: a model of type II diabetes

Rats that had been injected with monosodium glutamate (MSG) neonatally were studied for up to 70 weeks and compared with age‐matched control rats to study changes in glucose tolerance and in sympathetic and sensory nerves. At 61 and 65 weeks of age, there were significant differences in glucose tolerance between the MSG and control groups, and the MSG group had raised fasting blood glucose. These changes were not associated with changes in the number of β‐cells in the islets of Langerhans. In addition, the diabetic MSG‐treated rats had central obesity and cataracts. Hypoalgesia to thermal stimuli was present in MSG‐treated rats as early as 6 weeks and persisted at 70 weeks. However, no differences were observed in the distribution of substance P, the neurokinin‐1 receptor or calcitonin gene‐related peptide in the dorsal horn of L3–L5 at this age (70 weeks). Diabetic MSG‐treated animals at 65 and 70 weeks of age had significantly reduced noradrenaline concentrations in the heart, tail artery and ileum, while concentrations in the adrenal gland and corpus cavernosum were significantly increased. There was also a significant increase in adrenal adrenaline, dopamine and serotonin, largely attributable to changes in weight of the adrenal gland in the MSG‐treated animals. The results indicate that MSG‐treated animals develop a form of type II diabetes by about 60 weeks of age, and that there are significant changes in amine levels in various tissues associated with these developments.

The influence of visceral mechanoreceptors on sympathetic efferent discharge in the cat

1. Recordings have been made from eighty‐three single sympathetic efferent units in the hypogastric nerve in two types of preparation. In all animals the baroreceptors were denervated to exclude changes in sympathetic discharge resulting from any variations in arterial pressure, and the spinal cord was sectioned at the 6th lumbar segment to exclude changes in efferent discharge that might have been due to pelvic nerve afferents from the bladder or other viscera. In some animals the afferent pathways were sectioned from all pelvic and lower abdominal viscera other than the bladder, so that the vesical afferent pathway was the only neural pathway that might mediate reflex events from these viscera. The hypogastric nerve afferent pathway was excited by bladder distension or by bladder contractions induced by electrical stimulation of the sacral cord.

LONG-LASTING FACILITATION AND DEPRESSION OF PERIURETHRAL SKELETAL MUSCLE FOLLOWING ACUPUNCTURE-LIKE STIMULATION IN ANESTHETIZED RATS

The effects of acupuncture-like stimulation on the tone of the partially filled bladder and on the periurethral electromyogram (EMG) were examined in urethane-anesthetized rats. Acupuncture-like stimuli were usually applied to the skin and underlying muscles (or other structures), either separately or together, for a period of 1 min; the effects were studied in spinal cord intact and in spinalized animals. Maps have been constructed showing the effects of acupuncture-like stimulation at different sites on the body surface and of similar stimulation applied to individual muscles, the urethra and the testis. When acupuncture-like stimuli were applied to the skin and underlying structures, in the rostral half of the body and the hindpaw, testis or urethra, these stimuli usually induced excitation of periurethral EMG activity. Depression of EMG activity was seen predominantly during stimulation of structures close to the urethra, but not opposed to it. When acupuncture-like stimuli were applied only to structure beneath the skin, depression of EMG activity usually occurred. Acupuncture-like stimulation of the bulbocavernosus, which partly overlies the proximal urethra produced depression of EMG activity in 50% of trials, but the incidence of similar effects from the more distant pubococcygeus, or the dorsal or ventral sacrococcygeal muscles was about 90-100%. Acupuncture-like stimulation for 1 min could produce either excitation or depression of periurethral EMG activity lasting about 5 or 6 min, depending on the site of insertion and rotation of the acupuncture needles. Excitation of short duration (less than 3 min) was consistently observed from areas of the body distant to the bladder, i.e. the nose, forepaw, forelimb, chest, abdominal wall and hindpaw. Longer lasting excitation of EMG activity was often seen from the penile urethra, perineal area and hindlimb. Depression of EMG activity with a duration of more than 3 min was consistently seen from the muscles at the base of the tail (sacrococcygeus) and perineal area (pubococcygeus and bulbocavernosus). The bladder was partially filled in these experiments, so that micturition contractions were never seen; acupuncture-like stimulation of the perineal area induced some increase in bladder tone in 40% of trials. In spinalized animals, the pattern of activity induced by acupuncture-like stimulation was similar to that seen in spinal cord intact animals and the durations of the effects were not significantly different in these two groups. The distribution of sites from which acupuncture-like stimuli can influence the activity of the lower urinary tract is discussed.

Non-Markov negative correlation between interspike intervals in mammalian sympathetic efferent discharges

The background discharge of sympathetic efferent fibres in the hypogastric and splanchnic nerves of the cat was analyzed. Stationary discharges were renewal or had significant negative first order serial correlation coefficients. Negatively correlated discharges were non-Markov and the post-spike depression persisted for up to 5 s, covering the same time course as the prolonged inhibitory phenomena of sympathetic reflexes.

The nitric oxide synthase inhibitor l-NAME reduces inhibitory components of somato-vesical parasympathetic reflexes in the rat

Reflex discharges of pelvic postganglionic parasympathetic efferent fibers on the bladder surface induced by afferent volleys in the hindlimb nerve have been recorded in anesthetized rats, and the effects of the nitric oxide synthase inhibitor, N omega-nitro-L-arginine methyl ester (L-NAME) on the reflex discharges have been investigated. Single electrical stimulation of the tibial nerve at intensities supramaximal for excitation of A- and C-afferents evoked a reflex discharge in the postganglionic parasympathetic efferents with four distinct components, i.e., two inhibitory components with latencies of 49 and 203 ms, respectively, and two excitatory components with latencies of 126 and 308 ms, respectively. These reflexes could be observed when the bladder was expanded, but not markedly when the bladder was empty. Intravenous administration of L-NAME resulted in (a) a reduction in the level of resting discharge, (b) a reduction in the size of the first inhibitory component, (c) the disappearance of the second inhibitory component and (d) the exaggeration of the late excitatory component. Intracisternal injection of L-NAME caused changes similar to those observed following intravenous injection. The results suggest that inhibitory components of the somato-pelvic parasympathetic reflex are mediated by pathways that utilize nitric oxide as a neurotransmitter or neuromodulator at the level of the brainstem.