J. F. van den Bosch
University of Amsterdam
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by J. F. van den Bosch.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 1988
Elisa Garcia; Hans E. N. Bergmans; J. F. van den Bosch; I. Ørskov; B.A.M. van der Zeijst; Wim Gaastra
A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. These strains have been characterised with respect to their O, K, H, and fimbrial antigens, colicin production, antibiotic resistance, plasmid content and their ability to haemagglutinate erythrocytes from various species. Crossed immunoelectrophoresis of fimbrial extracts, as well as the reaction of partly purified fimbriae of a number of these strains with monoclonal antibodies revealed homology or a strong crossereaction with an F12 fimbrial subunit protein of human uropathogenic E. coli strains. Unlike human F12 fimbriae producing strains, the dog isolates did agglutinate dog erythrocytes in the presence of D-mannose but not human erythrocytes, indicating that the adhesin carried by these strains is different from the adhesin on fimbriae of human uropathogenic E. coli. Similar indications were obtained from experiments with latex beads coated with the receptor for P-fimbriae. These beads were agglutinated by Escherichia coli strains from human urinary tract infections, but not by the dog isolates described here. Preliminary adhesion experiments of human and dog Escherichia coli to human bladder epithelial and canine kidney epithelial cells also showed differences in adhesion depending on the origin of the strain tested.
Journal of Medical Microbiology | 1981
J. F. van den Bosch; Pieter W. Postma; J. de Graaff; D. M. Maclaren
The influence of haemolysin production on virulence was studied in an experimental mouse model. Urinary strains of Escherichia coli can be divided into three virulence groups by determining their kinetics in the mouse kidney after intravenous injection. Virulent strains of groups II and III were more often haemolytic than avirulent group-I strains. Haemolytic virulent strains often caused haemoglobinuria in the mice, and killed the mice more rapidly than did non-haemolytic virulent strains. No relationship was found between alpha-haemolytic activity and virulence in wild-type haemolytic strains. When haemolysin production was reduced or eliminated by treatment with actinomycin-D or rifampicin, six out of seven group-II strains tested gave the same results as avirulent group-I strains. However, the kinetics in the mouse kidney of four haemolytic group-III strains tested was not changed after reduction or elimination of haemolysin production; only a small decrease in toxicity was observed. It is concluded that haemolysin production by E. coli is a decisive virulence factor in most of the mouse-nephropathogenic group-II strains, but not in the virulent group-III strains.
Journal of Hygiene | 1982
J. F. van den Bosch; Pieter W. Postma; P. A. R. Koopman; J. de Graaff; D. M. MacLaren; D. van Brenk; P. A. M. Guinée
The virulence of faecal and urinary Escherichia coli strains was studied in relation to serotype, haemolysin production and haemagglutination pattern. By means of an experimental mouse model E. coli strains can be divided into avirulent (I), mouse nephropathogenic (II), and generally virulent (III) strains. Virulent group II and group III strains were more often haemolytic and haemagglutinating than avirulent group I strains. Presence of K antigen could not be associated with virulence. Discriminant analysis for qualitative variables revealed that no combination of the investigated properties contributed more to a strains virulence level than did one single property. It is concluded that other virulence factors, apart from haemolysin production in group II strains and haemagglutinins in group III strains, must be involved in the determination of a strains virulence level. All O2, O6 and O18 ac strains tested were virulent, and by far the most O75 strains were avirulent, whereas other O groups were more variable with regard to virulence. Pyelonephritis strains were more often mannose-resistance haemagglutinating than faecal and other urinary isolates, indicating that mannose-resistant adhesins may be important in the pathogenesis of pyelonephritis.
Journal of Medical Microbiology | 1983
A. M. J. J. Verweij-Van Vught; J. F. van den Bosch; F. Namavar; M. Sparrius; D. M. Maclaren
The importance of K antigens of Escherichia coli as virulence factors was studied by comparing groups of mice given either strains of E. coli isolated from urinary tract infection in humans or mutant strains differing only in the absence of the K antigen. K antigens proved to be of minor importance for mouse nephropathogenicity; however, with the exception of the K(A) antigen, they contributed substantially to deaths attributed to more general infection. Possible mechanisms for the virulence of strains with K antigens are discussed in terms of the bactericidal effect of serum and phagocytosis.
Microbial Pathogenesis | 1987
J. M. de Ree; Paul Schwillens; J. F. van den Bosch
Pap fimbriae were purified from a recombinant strain and used for the production of monoclonal antibodies (MAbs). These MAbs were screened in a fimbriae ELISA with eight different P fimbriae as well as 1A and 1C fimbriae. Five MAbs were specific for Pap fimbriae whereas one MAb did react with Pap, F7(2) and F11 fimbriae. Previously, we described two F11 MAbs which also reacted with Pap, F7(2) and F11 fimbriae. In a whole bacteria ELISA it was shown that the MAbs, which recognized Pap, F7(2) and F11 fimbriae, reacted with recombinant strains which did not express Pap or F11 fimbriae, but still expressed the globoside binding properties. Not one of the five MAbs which are specific for Pap fimbriae reacted with these globoside binding recombinant strains. In a haemagglutination and adherence assay it was shown that only the MAbs which recognized the Pap, F7(2) and F11 fimbriae inhibited the adhesive properties of the globoside binding recombinant strain. Therefore it is concluded that in the present study MAbs are presented which recognize the minor components responsible for adhesion.
Journal of Medical Microbiology | 1989
J. M. de Ree; J. F. van den Bosch
Surmmary Strains of Escherichia coli isolated from urinary tract infections and meningitis were characterised by their O:K serotype, haemolysin production, mannose-resistant haemagglutination, and the serotype of the P-fimbriae. The P-fimbriae of 71% of the mannose-resistant haemagglutination-positive strains from urinary tract infection and meningitis could be determined with specific monoclonal antibodies. Many strains expressed multiple P-fimbriae serotypes. The serotypes of P-fimbriae found most frequently among mannose-resistant haemagglutination-positive E. coli from urinary tract infections were the F11, F71 and F8 fimbriae, and among meningitic strains, F11, F8 and F9 fimbriae. The expression of certain F-serotypes did not correlate with O:K antigens.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 1980
J. F. van den Bosch; P. L. Oe; Pieter W. Postma; J. de Graaff; D. M. MacLaren; W. H. Jansen; P. A. M. Guinée
E. coli strains were isolated from urine specimens of hospitalised patients with acute pyelonephritis, acute cystitis or asymptomatic bacteriuria (ABU), and tested for virulence in an experimental mouse model. Of 12 pyelonephritisstrains 11 were shown to be virulent and 1 avirulent; of 12 cystitis-strains 4 were virulent and 8 avirulent; of 12 ABU-strains 5 were virulent and 7 avirulent. It is concluded that, while no difference in virulence was found between cystitis-and ABU-strains, pyelonephritis-strains were more often virulent than cystitis-and ABU-strains.No associations could be shown between virulence of the isolated strains and the presence of antibody-coated bacteria in the urine. Common urinary O types were not more often virulent than other O types. No relationship was seen between virulence and the presence of K antigen or the presence of particular K types.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 1981
J. F. van den Bosch; Pieter W. Postma; D. van Brenk; P. A. M. Guinée; J. de Graaff; D. M. MacLaren
E. coli strains were isolated from the urine of patients with acute cystitis in general practice and from the faeces of a comparable reference group of healthy individuals. These strains were serotyped and tested for virulence in an experimental mouse model. Of 30 cystitis-strains 18 were virulent, and of 30 faeces-strains 15 were virulent.It is concluded that the cystitis-strains were not more often virulent than the faeces-strains.O antigens commonly found among urinaryE. coli isolates were present in 60% of the cystitis-strains and in 37% of the faeces-strains. K antigens commonly found in urinaryE. coli strains were present in 33% of the cystitis-strains and in 12% of the faeces-strains. Neither the presence of common urinary O-antigens, nor the presence of common urinary K antigens could be associated with virulence of the isolated strains. However, it is suggested that certain O and K antigens (O2, O6, K23) may be associated with virulence for the urinary tract.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 1985
A. C. J. M. Antonissen; P. J. M. R. Lemmens; R. Gonggrijp; J. F. van den Bosch; C. P. A. van Boven
Crude ribosomes were isolated fromListeria monocytogenes serotype 4b and separated into two fractions by molecular sieve chromatography. Chemical analysis indicated that fraction I contained cell envelope components while fraction II contained the ribosomes. Both fractions protected mice againstListeria, but only in combination with the adjuvant dimethyldioctadecylammonium bromide (DDA). RNase-treatment; but not proteinase K-treatment destroyed the protective properties of fraction II, and RNA purified from fraction II also induced protection. Protection induced by fraction I was not affected by either RNase- or proteinase K-treatment. Both subcutaneous and intraperitoneal, but not intravenous administration of fraction I, fraction 11, or purified RNA induced significant protection against intraperitoneal infection, the intraperitoneal route of administration being the most effective. All preparations induced high levels of protection 3 to 7 days after administration, but protection was already decreased after 14 days. Protection induced with RNA appeared to be biphasic, because it also protected mice 1 day, but not 2 days after administration. Protection induced with both fraction I and RNA was at least in part nonspecific, because both preparations also protected mice againstL. monocytogenes serotype 3,Streptococcus pneumoniae andPseudomonas aeruginosa. Results are discussed in relation to previous work with analogous preparations fromP. aeruginosa.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 1984
R. Gonggrijp; A. C. J. M. Antonissen; J. F. van den Bosch; C. P. A. van Boven
This paper presents an analysis of the protective properties of the components in ribonuclease (RNase)-sensitive ribosomal vaccines, in particular the ribonucleic acid (RNA). The protective activities in mice of purified ribosomes derived fromPseudomonas aeruginosa and fromListeria monocytogenes were compared. Both ribosomal vaccines had to be combined with the adjuvant dimethyldioctadecylammonium bromide (DDA) in order to be protective, and both lost their activity after RNase treatment. The ribosomal vaccines as well as RNA purified from the ribosomes induced non-specific protection. Intraperitoneal injection of RNA with DDA induced an influx of peritoneal cells. Furthermore, RNA with DDA activated macrophages as shown by, a.o., enhanced phagocytic activity and killing capacity forL. monocytogenes. The results suggest that the observed macrophage activation is probably T-cell-independent. With regard to the ribosomal vaccine ofP. aeruginosa it is concluded that RNA also contributed to the protective activity by increasing the humoral response against suboptimal concentrations of contaminating cell surface antigens.In conclusion, it is proposed that ribosomal vaccines may be considered as a combination of a non-specific immunomodulator (RNA) with pathogen-specific cell surface antigens. This concept of ribosomal vaccines is discussed in relation to the literature concerning RNase-sensitive ribosomal vaccines.