J.J. Gamero

Y-chromosome STR haplotypes in a southwest Spain population sample

The Y-chromosome polymorphism of eight STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392; DYS393, DYS385) were studied in 111 unrelated individuals from the population of southwest Spain. The most common haplotype was shared by 3.6% of the sample, while 99 haplotypes were unique. The gene diversity was 0.9977.

A study of Spanish attitudes regarding the custody and use of forensic DNA databases.

One of the issues that has resulted in much disagreement in many countries at different levels concerns the kind of institution that should be given the responsibility of exercising custody over biological samples and the DNA profiles obtained from these samples. In the field of forensic genetics, there is no doubt that the existence of DNA criminal databases benefits the control and investigation of crime. However, certain criticism, supported to a great extent by the particular vision of genetic exceptionalism has been aimed at the ethical and social consequences resulting from the inappropriate use of such databases. In this sense, it was stated that the support of the population was required for those regulations that propose the extension of police powers in the collection and storage of biological samples, as well as their corresponding DNA analyses. Without such backing, such measures may cause society to distrust the nature of the protection afforded by the legal system and be interpreted as interference in the civil liberties and human rights of the individual. We believe that the opinion poll which has been carried out among the Spanish population may serve to reveal the public attitudes/criteria which society has with regard to those institutions responsible for the custody of DNA profile databases. Finally, it must be pointed out that when the interviewees were asked about what institution or institutions should protect and maintain data confidentiality 59.7% considered that custody should remain in the hands of the National Agency for DNA Profiles (a judicially backed, autonomous public institution).

Y-chromosome STR haplotypes in two population samples: Azores Islands and Central Portugal

The Y-chromosome haplotypes defined by nine STRs (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392 and DYS393) were studied in 207 unrelated individuals from Central Portugal and 63 from Azores Islands. The most common haplotype in Central Portugal was shared by 3.4% of the males, while 160 haplotypes were unique. In Azores Islands the most common haplotype was shared by 6.4% of the males, while 40 haplotypes were unique. The values of haplotype diversity were 0.993 for Central Portugal and 0.976 for Azores Islands.

A study on ten short tandem repeat systems: African immigrant and Spanish population data.

This work presents the results obtained from a genetic-population study for the D1S1656 system in the population of Southwest Spain (Huelva, Cádiz and Sevilla), Spaniards of Caucasian origin from North Africa (Ceuta), as well as in the black Central West African and Moroccan immigrant populations in Spain. The results of a study of the autochtonous population of the Canary Islands (n=138), and immigrant Central West African populations in Spain (n=132), obtained for nine short tandem repeat (STR) loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820), as well as the amelogenin locus, all contained in Profiler Plus (Perkin-Elmer) PCR amplification kits, are also presented. Except for the FGA and VWA data on immigrant Central West African populations in Spain, no deviations from the Hardy-Weinberg equilibrium were detected.

Y-chromosome STR haplotypes in Central-West African immigrant in Spain population sample.

Blood samples were obtained from 103 healthy unrelated blackWest African immigrant population in Spain from different coun-tries situated to the south of the Sahara desert. The DNA was ex-tracted using Chelex 100 protocol as described by Walsh et al. (1). The quantity of recovered DNA was determined using Quan-tiBlot® Human DNA Quantitation Kit (Perkin Elmer). DYS19,DYS389I, DYS389II, DYS390, and DYS393 were amplified asdescribed by Gusmao et al. (2). The DYS385 amplification con-ditions complied with the methodology described by Schneider etal. (3). The PCR was performed in a Perkin Elmer 9600 thermo-cycler.Allele nomenclature was proposed by Kayser et al. (4) and deKnijff (5) with the exception of the DYS389 locus. The nomencla-ture of this locus was according Gusmao et al. (2).Electrophoresis was carried out on 4% polyacrylamide dena-turing sequencing gels in a 377 automated system (AppliedBiosystems Division/Perkin Elmer). Genotype classification wasdone using Genescan PCR analysis software with Local SouthernMethod and by side-to-side comparison with allelic ladders.These ladders were kindly provided by Dr. Carracedo (Institute ofLegal Medicine of Santiago de Compostela), Dr. P.M. Schneider(Institut fur Rechtsmedizin, Mainz, Germany). Haplotype diver-sity was calculated according to Nei (6). Analysis of MolecularVariance (AMOVA) was performed by Markov test using the Ar-lequin software 1.1 (7). Genetic distance matrix between popula-tions were obtained by using the pairwise difference genetic dis-tance.A total of 98 different haplotypes were observed (Table 1), 93 ofthem being unique. The most common haplotype (DYS19: 15,DYS385: 16/16, DYS389I: 10, DYS389II: 28, DYS390: 21, andDYS393: 13) had frequency of 1.94% (h16, h21, h22, h38, andh54). Haplotype diversity for each of the markers is shown in Table1. Haplotype diversity for all seven Y-specific STR loci in Central-West African Immigrant in Spain population was calculated to be0.9990 (standard error: 0.0015).Pairwise haplotype analysis using the population studied andother Iberian andAfrican populations (8,9) shows the valencian (

A population-genetic study of the DYS385 haplotypes in two Spanish populations and the African immigrant population in Spain.

A Population-Genetic Study of the DYS385 Haplotypes in Two Spanish Populations and the African Immigrant Population in Spain

Analysis of Y-chromosomal haplotypes and haplogroups distribution in a population sample from Portugal (central area)

Abstract The aim of this study was to analyse the distribution of Y-chromosomal haplotypes and haplogroups found in central Portugal. In this work, we analysed 102 unrelated individuals of central Portugal. Combining the allelic state of 10 biallelic markers (YAP, SRY-8299, 92R7, 12f2, SRY-1532, SRY-2627, Tat, SY81, M9, LLY22g), we defined the haplogroup to which each sample belonged. Y-chromosomal haplotypes were defined by 16 Y-Short Tandem Repeats (STR) (DYS19, DYS3855 a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4 and GATA H4). This population study defined 8 different haplogroups and 101 different haplotypes, where 100 haplotypes were unique and 1 was found in two apparently unrelated individuals, both belonging to the same haplogroup.

Y-chromosome STRs DYS385, DYS19, DYS389-I and II, DYS390, DYS391, DYS392 and DYS393 in five African populations

Background: The Y chromosome has been used to compare the relationship between populations, representing a rich source of potential information to trace paternal lineages and providing a record of our relatedness. Among different population groups, African populations seem to be very interesting to study, considering the theory of the origin of modern humans and the ethnic variability usually existing. Methods: Five male populations from Angola (n=48), Cap Verde (n=47), GuineaBissau (n=32), Mozambique (n=36) and S. Tome and Prince (n=30) were studied for Ychromosome Short Tandem Repeats (STRs) DYS385, in addition to DYS19, DYS389-I and II, DYS390, DYS391, DYS392 and DYS393 previously searched. Results: The high DYS385 polymorphism confers a considerable variability to the Y-chromosome haplotype. Conclusions: The comparisons between the African populations and other data, namely from a Portuguese population, showed an interesting location of insular populations, with an intermediate position between Caucasoid and African continental populations, probably due to the European presence in the colonization of those islands. D 2003 Elsevier Science B.V. All rights reserved.

A study on four short tandem repeat systems: African immigrant, Portuguese, and Spanish population data.

POPULATION: Portuguese, Spaniards from the southwest of Spain, Spaniards of Caucasian origin from North Africa (Ceuta), immigrants from Morocco and immigrants from West Africa.