J.M. Maia

The Role of Vitamins in Cancer: A Review

Vitamins are essential nutrients for human metabolism, playing an important role as coenzymes or enzymes in many vital processes for the normal functioning of the body. In recent years, it has become apparent that vitamins are crucial in health and human disease, due to several studies that studied this relationship. Currently, it is known that vitamins can have an important role in the prevention and treatment of cancer, but until now no conclusive results were obtained. In this review, we will present the work and more relevant conclusions obtained in recent years of investigation about the relationship between vitamins and cancer, namely vitamin A, vitamin B complex, vitamin C, vitamin D, vitamin E, and vitamin K.

XIPE: the X-ray imaging polarimetry explorer

Abstract X-ray polarimetry, sometimes alone, and sometimes coupled to spectral and temporal variability measurements and to imaging, allows a wealth of physical phenomena in astrophysics to be studied. X-ray polarimetry investigates the acceleration process, for example, including those typical of magnetic reconnection in solar flares, but also emission in the strong magnetic fields of neutron stars and white dwarfs. It detects scattering in asymmetric structures such as accretion disks and columns, and in the so-called molecular torus and ionization cones. In addition, it allows fundamental physics in regimes of gravity and of magnetic field intensity not accessible to experiments on the Earth to be probed. Finally, models that describe fundamental interactions (e.g. quantum gravity and the extension of the Standard Model) can be tested. We describe in this paper the X-ray Imaging Polarimetry Explorer (XIPE), proposed in June 2012 to the first ESA call for a small mission with a launch in 2017. The proposal was, unfortunately, not selected. To be compliant with this schedule, we designed the payload mostly with existing items. The XIPE proposal takes advantage of the completed phase A of POLARIX for an ASI small mission program that was cancelled, but is different in many aspects: the detectors, the presence of a solar flare polarimeter and photometer and the use of a light platform derived by a mass production for a cluster of satellites. XIPE is composed of two out of the three existing JET-X telescopes with two Gas Pixel Detectors (GPD) filled with a He-DME mixture at their focus. Two additional GPDs filled with a 3-bar Ar-DME mixture always face the Sun to detect polarization from solar flares. The Minimum Detectable Polarization of a 1 mCrab source reaches 14 % in the 2–10 keV band in 105 s for pointed observations, and 0.6 % for an X10 class solar flare in the 15–35 keV energy band. The imaging capability is 24 arcsec Half Energy Width (HEW) in a Field of View of 14.7 arcmin × 14.7 arcmin. The spectral resolution is 20 % at 6 keV and the time resolution is 8 μs. The imaging capabilities of the JET-X optics and of the GPD have been demonstrated by a recent calibration campaign at PANTER X-ray test facility of the Max-Planck-Institut für extraterrestrische Physik (MPE, Germany). XIPE takes advantage of a low-earth equatorial orbit with Malindi as down-link station and of a Mission Operation Center (MOC) at INPE (Brazil). The data policy is organized with a Core Program that comprises three months of Science Verification Phase and 25 % of net observing time in the following 2 years. A competitive Guest Observer program covers the remaining 75 % of the net observing time.

Advances in the Micro-Hole & Strip Plate gaseous detector

We report on the performance of a new gaseous electron multiplier: the Micro-Hole & Strip Plate (MHSP). It consists of two independent charge-amplification stages in a single, double-sided micro-structured electrode, deposited on a thin insulating substrate. Charge gains in excess of 10 3 were obtained in a MHSP operated with soft X-rays in Ar/CO2 (70/ 30). We present the results of a systematic study of the MHSP properties and those of a double-stage GEM+MHSP multiplier. Applications to gaseous photomultipliers are discussed. r 2003 Elsevier Science B.V. All rights reserved. PACS: 29.40.� n; 29.40.Cs; 85.60.Gz

Ion-induced effects in GEM and GEM/MHSP gaseous photomultipliers for the UV and the visible spectral range

We report on the progress in the study of cascaded GEM and GEM/MHSP gas avalanche photomultipliers operating at atmospheric pressure, with CsI and bialkali photocathodes. They have single-photon sensitivity, ns time resolution and good localization properties. We summarize operational aspects and results, with the highlight of a high-gain stable gated operation of a visible-light device. Of particular importance are the results of a recent ion-backflow reduction study in different cascaded multipliers, affecting the detectors stability and the photocathodes lifetime. We report on the significant progress in ion-blocking and provide first results on bialkali-photocathode aging under gas multiplication.

THGEM operation in Ne and Ne/CH4

The operation of Thick Gaseous Electron Multipliers (THGEM) in Ne and Ne/CH4 mixtures, features high multiplication factors at relatively low operation potentials, in both single- and double-THGEM configurations. We present some systematic data measured with UV-photons and soft x-rays, in various Ne mixtures. It includes gain dependence on hole diameter and gas purity, photoelectron extraction efficiency from CsI photocathodes into the gas, long-term gain stability and pulse rise-time. Position resolution of a 100 × 100 mm2 X-ray imaging detector is presented. Possible applications are discussed.

Cytotoxicity of Ascorbic Acid in a Human Colorectal Adenocarcinoma Cell Line (WiDr): In Vitro and In Vivo Studies

Vitamin C, available in its reduced form (ascorbic acid; AA) and in its oxidized form (dehydroascorbic acid; DHA), may act in physiological conditions as an antioxidant or pro-oxidant. The aim of this study is to evaluate the cytotoxic effects of pharmacological doses of AA in a human colorectal adenocarcinoma cell line (WiDr) in vitro, through spectrophotometry, clonogenic assays and flow cytometry, and in vivo with xenotransplanted Balb/c nu/nu mice. The results show that the reduced form of vitamin C induces an anti-proliferative and cytotoxic effect in adenocarcinoma colorectal cells under study. The results obtained in vivo after treatment with AA showed a large reduction in the rate of tumor growth. Such understanding can guide decisions about which colorectal cancer patients might potentially benefit from vitamin C pharmacologic therapy.

Operation of a Thick Gas Electron Multiplier (THGEM) in Ar, Xe and Ar-Xe

We present the results of our recent studies of a Thick Gaseous Electron Multiplier (THGEM)-based detector, operated in Ar, Xe and Ar:Xe (95:5) at various gas pressures. Avalanche-multiplication properties and energy resolution were investigated with soft x-rays for different detector configurations and parameters. Gains above 104 were reached in a double-THGEM detector, at atmospheric pressure, in all gases, in almost all the tested conditions; in Ar:Xe (95:5) similar gains were reached at pressures up to 2 bar. The energy resolution dependence on the gas, pressure, hole geometry and electric fields was studied in detail, yielding in some configurations values below 20% FWHM with 5.9 keV x-rays.

Recent investigations of cascaded GEM and MHSP detectors

We present results from our recent investigations on detectors comprising cascaded gas electron multipliers (GEM) and cascaded GEMS with micro-hole and strip (MHSP) electrodes as a final amplification stage. We discuss the factors governing the operation of these fast radiation-imaging detectors, which have single-charge sensitivity. The issue of ion-backflow and ion-induced secondary effects is discussed in some detail, presenting ways for its suppression. Applications are presented in the fields of photon imaging in the UV-to-visible spectral range as well as x-ray and neutron imaging.

MHSP in reversed-bias operation mode for ion blocking in gas-avalanche multipliers

We present recent results on the operation of gas-avalanche detectors comprising a cascade of gas electron multipliers (GEMs) and Micro-Hole and Strip Plates (MHSPs) multiplier operated in reversed-bias (R-MHSP) mode. The operation mechanism of the R-MHSP is explained and its potential contribution to ion-backflow (IBF) reduction is demonstrated. IBF values of 4E-3 were obtained in cascaded R-MHSP and GEM multipliers at gains of about 1E+4, though at the expense of reduced effective gain in the first R- MHSP multiplier in the cascade.

Single-Electron Response Using a GEM-MIGAS Electron Multiplier

A gas electron multiplier with a micro-induction gap amplifying structure (GEM-MIGAS) is formed when a conventional GEM is operated with a short induction gap, typically set at 50 mum. Experimental studies were carried out to investigate the single-electron response of a GEM-MIGAS, using a He/iso-C4H10 (85/15%) gas mixture operated in a flow mode at atmospheric pressure. The additional charge multiplication in the induction gap results in a gain increase up to one order of magnitude when compared to the GEM mode operation alone. A series of measurements were undertaken to examine the pulse height distributions induced by single-electrons under a wide range of bias voltages applied across the GEM holes, 100 to 550 V, and for a wide range of electric fields in the induction gap, 0.6 to 100 kV/cm. It was possible to sustain effective charge gains in excess of 3times105 B and multiplication relative variances around 0.4 over a large range of GEM voltages, enabling us to demonstrate single-electron detection efficiencies above 98%.