J.P. Allen

Overview and Design Biospherics and Biosphere 2, mission one (1991–1993)

Abstract This paper outlines concepts, construction and operation of Biosphere 2, the large glass closed life facility in the mountains of southern Arizona, USA. Plans used concepts of systems ecology and biospherics from the early writings of V.I. Vernadsky, work of the Russian space program on closed ecological life support systems and other leading proponents of a total systems approach to ecology. Mission one was the first experimental closure of Biosphere 2 with eight crew members for 2 years, 1991–1993.

Earth applications of closed ecological systems : relevance to the development of sustainability in our global biosphere

The parallels between the challenges facing bioregenerative life support in artificial closed ecological systems and those in our global biosphere are striking. At the scale of the current global technosphere and expanding human population, it is increasingly obvious that the biosphere can no longer safely buffer and absorb technogenic and anthropogenic pollutants. The loss of biodiversity, reliance on non-renewable natural resources, and conversion of once wild ecosystems for human use with attendant desertification/soil erosion, has led to a shift of consciousness and the widespread call for sustainability of human activities. For researchers working on bioregenerative life support in closed systems, the small volumes and faster cycling times than in the Earths biosphere make it starkly clear that systems must be designed to ensure renewal of water and atmosphere, nutrient recycling, production of healthy food, and safe environmental methods of maintaining technical systems. The development of technical systems that can be fully integrated and supportive of living systems is a harbinger of new perspectives as well as technologies in the global environment. In addition, closed system bioregenerative life support offers opportunities for public education and consciousness changing of how to live with our global biosphere.

INITIAL EXPERIMENTAL RESULTS FROM THE LABORATORY BIOSPHERE CLOSED ECOLOGICAL SYSTEM FACILITY

An initial experiment in the Laboratory Biosphere facility, Santa Fe, New Mexico, was conducted May-August 2002 using a soil-based system with light levels (at 12 h per day) of 58-mol m-2 d-1. The crop tested was soybean, cultivar Hoyt, which produced an aboveground biomass of 2510 grams. Dynamics of a number of trace gases showed that methane, nitrous oxide, carbon monoxide, and hydrogen gas had initial increases that were substantially reduced in concentration by the end of the experiment. Methane was reduced from 209 ppm to 11 ppm, and nitrous oxide from 5 ppm to 1.4 ppm in the last 40 days of the closure experiment. Ethylene was at elevated levels compared to ambient during the flowering/fruiting phase of the crop. Soil respiration from the 5.37 m2 (1.46 m3) soil component was estimated at 23.4 ppm h-1 or 1.28 g CO2 h-1 or 5.7 g CO2 m-2 d-1. Phytorespiration peaked near the time of fruiting at about 160 ppm h-1. At the height of plant growth, photosynthesis CO2 draw down was as high as 3950 ppm d-1, and averaged 265 ppm h-1 (whole day averages) during lighted hours with a range of 156-390 ppm h-1. During this period, the chamber required injections of CO2 to continue plant growth. Oxygen levels rose along with the injections of carbon dioxide. Upon several occasions, CO2 was allowed to be drawn down to severely limiting levels, bottoming at around 150 ppm. A strong positive correlation (about 0.05 ppm h-1 ppm-1 with r2 about 0.9 for the range 1000-5000 ppm) was observed between atmospheric CO2 concentration and the rate of fixation up to concentrations of around 8800 ppm CO2.

Closed Ecological Systems, Space Life Support and Biospherics

This chapter explores the development of a new type of scientific tool – man-made closed ecological systems. These systems have had a number of applications within the past 50 years. They are unique tools for investigating fundamental processes and interactions of ecosystems. They also hold the potentiality for creating life support systems for space exploration and habitation outside of Earth’s biosphere. Finally, they are an experimental method of working with small “biospheric systems” to gain insight into the functioning of Earth’s biosphere. The chapter reviews the terminology of the field, the history and current work on closed ecological systems, bioregenerative space life support and biospherics in Japan, Europe, Russia, and the United States where they have been most developed. These projects include the Bios experiments in Russia, the Closed Ecological Experiment Facility in Japan, the Biosphere 2 project in Arizona, the MELiSSA program of the European Space Agency as well as fundamental work in the field by NASA and other space agencies. The challenges of achieving full closure, and of recycling air and water and producing high-production crops for such systems are discussed, with examples of different approaches being used to solve these problems. The implications for creating sustainable technologies for our Earth’s environment are also illustrated.

Soil in the agricultural area of Biosphere 2 (1991–1993)

Abstract The agricultural area (intensive agriculture biome) of Biosphere 2 was started with a meter of alkaline soil with high organic content in 1990 and used to support eight persons during closure 1991–1993. Wastes were recycled to maintain fertility. Soils sampled at the end were analyzed for major and minor inorganic constituents and organic matter. C/N ratio was high (13:1 to 17:1). Organic matter decreased 0.2 to 1.4% per year. With exception of high salinity developing in one plot and denitrification in the rice paddy, the soils at the end were functional in supporting crop production.

Effects of a Spaceflight Environment on Heritable Changes in Wheat Gene Expression

Once it was established that the spaceflight environment was not a drastic impediment to plant growth, a remaining space biology question was whether long-term spaceflight exposure could cause changes in subsequent generations, even if they were returned to a normal Earth environment. In this study, we used a genomic approach to address this question. We tested whether changes in gene expression patterns occur in wheat plants that are several generations removed from growth in space, compared to wheat plants with no spaceflight exposure in their lineage. Wheat flown on Mir for 167 days in 1991 formed viable seeds back on Earth. These seeds were grown on the ground for three additional generations. Gene expression of fourth-generation Mir flight leaves was compared to that of the control leaves by using custom-made wheat microarrays. The data were evaluated using analysis of variance, and transcript abundance of each gene was contrasted among samples with t-tests. After corrections were made for multiple tests, none of the wheat genes represented on the microarrays showed a statistically significant difference in expression between wheat that has spaceflight exposure in their lineage and plants with no spaceflight exposure. This suggests that exposure to the spaceflight environment in low Earth orbit space stations does not cause significant, heritable changes in gene expression patterns in plants.

Designing a Small-scale Infra-free (IF) System for Community Applications: Managing Energy, Water and Waste

abstract This paper explores alternative infra(structure)-free (IF) scenarios at a community level to promote machi-zukuri (community/neighborhood planning), a bottom-up decentralization approach improving citizen’ and municipality involvement in city planning in Japan. Demographic analysis in Japan shows that the population is becoming more urbanized, with an increasingly centralized infrastructure, but that per capita waste generation is increasing because the number of people in each household is decreasing; therefore, integration of the energy, water and waste (EWW) cycles becomes more important. For residents who are unconnected to centralized sewage treatment in Japan, mainly concentrated in municipalities whose population is less than 100,000, there is a lack of alternatives for wastewater treatment, except the current technically-demanding ‘joukasou’ on-site treatment system. The authors evaluated the 30-year life-cycle cost performance of three current systems with alternative (integrated-technology) IF scenarios focusing on wastewater treatment for a small community (20 households). These systems are; wastewater gardens with biogas production, an anaerobic digester gas system integrated with fuel cell technology and a heat and power unit (CHP) combined with a biogas-producing reed bed system, all of which treat wastewater and result in useful end products-, closing the life cycle with low maintenance, a lower environmental load-, and two to four times smaller development cost than centralized options in both rural and urban communities.