J. P. Minyard

Reaction chromatography : I. Gas-liquid/thin-layer chromatographic derivatization technique for the identification of carbonyl compounds☆

Abstract The technique for the identification of reactive compounds by derivative formation on thin-layer plates at the exhaust of a gas chromatograph (gas-liquid/thin-layer chromatography) was applied to alcohols. Advantages, limitations, and reaction conditions for 3,5-dinitrobenzoates (DNBs) and o -nitrophenylurethans (ONPUs) as derivatives of representative alcohols used in this study are discussed and thin-layer chromatographic behavior in several systems is tabulated. DNBs are readily formed from 0.5 mg and ONPUs from 0.05 mg of the same primary and secondary alcohols. Some tertiary alcohols failed to give either derivative from 10 mg of reactant, while 0.5–5 mg of other reacted discernibly.

Constituents of the cotton bud—VII. : Identification of the anthocyanin as chrysanthemin☆

Abstract The only anthocyanin m cotton buds and flowers of Gossypium hirsutum L., var. Delta Pine Smooth Leaf, was identified as chrysanthemin, the 3-β-monoglucoside of cynidin. Buds and flowers contained 0-030 and 0-041 per cent pigment, respectively.

Constituents of the cotton bud: XII. The carotenoids in buds, seeds and other tissue.

Eleven carotenoid pigments were found in the bud, leaf, flower petal, seedling and seed of the cotton plant; nine were identified and quantitated. The most abundant carotenoids in the green tissue of the cotton plant were β-carotene and lutein. Carotene hydrocarbons comprised 12% of the total carotenoids in the seed, 15% in the 1-day-old flower petal, 51% in the bud and 57% in green leaves. Only 5,8-epoxy carotenoids were found in the flower petals and only 5,6-epoxides in the other tissue but both were present in the seed. The colorless phytoene precursors to the carotenoids comprised from 20% to 38% of the total carotenoid pigment in the growing tissue of the plant.