J.R. Reddan

The Control of Cell Division in the Ocular Lens

Publisher Summary This chapter discusses the control of cell division in the ocular lens and primarily deals with the nature of the effects of environmental factors on the control of cell division. In certain very important related areas, such as differentiation, regeneration, and aging, the lens has proved to be the most valuable model system for analysis. These active areas of investigation, including studies on cataract formation are addressed in the chapter. Moreover, emphasis is placed on concepts of the mechanisms that underlie the control of cell division, particularly as they apply to the ocular lens. It is assumed that the knowledge gained for one tissue on a fundamental mechanism such as the control of cell division, may apply, at least in part, to control mechanisms in other tissues.

An enzymatic assay for DNA in epoxy-tissue-sections by light microscope-autoradiography

Thick and thin sections of glutaraldehyde- and osmium-fixed and epoxy-embedded tissues were incubated with calf-thymus terminal deoxynucleotidyl transferase, and the resulting incorporation of 3H-dAMP was located by light microscope-autoradiography. This cyto-enzymatic method is rapid and permits detection of nuclear DNA in a large variety of cell types from the rat trachea, rabbit lens epithelium, and newt ovaries.

Regression of Cataracts in the Offspring of Galactose Fed Rats

3 days after conception, pregnant Sprague-Dawley rats were fed a diet of galactose + ground lab chow, glucose + ground lab chow, or ground lab chow alone. After delivery, mothers were fed lab chow alo

Experimental Modification of the Temporal Aspects of the Serum-Induced Cell Cycle in the Cultured Adult Rabbit Lens

The adult rabbit lens in organ culture has been used as a model system for studies on the control of cell division in an organized tissue. Freshly isolated lenses, placed immediately in culture media