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Featured researches published by J. S. Otis.


Theoretical and Applied Genetics | 1990

The improbability of irradiated spermatozoa as gene transfer vectors in chickens

Robert N. Shoffner; J. S. Otis; L. A. Snyder; K. S. Guise

SummaryIt is concluded that chromatin fragments derived from irradiated chicken spermatozoa are not viable vectors for gene transfer. In three experiments conducted at sequential intervals over a period of 1 1/2 years, no marker traits were found in 1,065 G0 progeny from irradiated spermatozoa of Minnesota Dominant Marker males inseminated into recessive Rhode Island Red and White Leghorn females. The inability to secure transformants is ascribed to the following factors: a maximum of five and probably fewer potential vector fragments for each G0 progeny because of irradiation effect on spermatozoan ability to enter the germinal disc; uncertainty of DNA integrity from highly irradiated chromatin; no known mechanism for release of chromatin fragments from irradiated spermatozoa supernumerary pronuclei; and the uncertainty of selective integration into the zygotic nucleus.


Cytogenetic and Genome Research | 1981

A cyclical translocation, t(1;8;5), in the domestic chicken (Gallus domesticus).

J. J. Bitgood; J. S. Otis; Robert N. Shoffner; N.S. Fechheimer

A chromosome analysis of a translocation in the domestic chicken ( Gallus domesticus ) revealed that it was a cyclical translocation involving chromosomes 1, 5, and 8. Meiotic prepara


Poultry Science | 1967

Avian Chromosome Methodology

Robert N. Shoffner; Awtar Krishan; G. J. Haiden; R. K. Bammi; J. S. Otis


Poultry Science | 1990

Research Note: Cryopreservation of Semen from Unique Lines of Chicken Germ Plasm

A. Tajima; E.F. Graham; Robert N. Shoffner; J. S. Otis; D. M. Hawkins


Poultry Science | 1980

Mapping of the Genes for Pea Comb, Blue Egg, Barring, Silver, and Blood Groups A, E, H, and P in the Domestic Fowl

J. J. Bitgood; Robert N. Shoffner; J. S. Otis; W. E. Briles


Poultry Science | 1982

The Effect of a Protoporphyrin Mutant on Some Economic Traits of the Chicken

Robert N. Shoffner; R. Shuman; J. S. Otis; J. J. Bitgood; V. A. Garwood; P. C. Lowe


Poultry Science | 1993

Association of Dominant Marker Traits and Metric Traits in Chickens

Robert N. Shoffner; J. S. Otis; V. A. Garwood


Poultry Science | 1983

Refined Linkage Value for Pea Comb and Blue Egg: Lack of Effect of Pea Comb, Blue Egg, and Naked Neck on Age at First Egg in the Domestic Fowl

J. J. Bitgood; J. S. Otis; Robert N. Shoffner


Poultry Science | 1996

Gene Mapping by Chromosome Microdissection and Microisolation in the Chicken

E. M. Shaw; J. S. Otis; S. J. Lamont; K. S. Guise; Robert N. Shoffner


Journal of Heredity | 1991

Estimating Meiotic Disjunction Frequencies in Chicken Translocation Heterozygotes Based on Embryonic Mortality

J. J. Bitgood; Robert N. Shoffner; J. S. Otis

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J. J. Bitgood

University of Wisconsin-Madison

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K. S. Guise

University of Minnesota

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E. M. Shaw

University of Minnesota

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A. Tajima

University of Minnesota

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E.F. Graham

University of Minnesota

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G. J. Haiden

University of Minnesota

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