J. San Román

Observation of spontaneous self-channeling of light in air below the collapse threshold

We report the observation of the self-guided propagation of 120 fs, 0.56 mJ infrared radiation in air for distances greater than 1 m. In contrast with the known case of filamentation, in the present experiment the laser power is lower than the collapse threshold. Therefore the counterbalance between Kerr self-focusing and ionization induced defocusing as the stabilizing mechanism is ruled out. Instead, we find evidence of a process in which the transversal beam distribution reshapes into a form similar to a Townes soliton, with the particularity of a very high stability. We include numerical support for this conclusion.

Self-compression controlled by the chirp of the input pulse

Self-compressed (SC) pulses have been achieved through the filamentation process in air without any additional dispersion compensation, using the input pulse chirp as the control parameter. For any studied input pulse energy (3-5 mJ), we have found two opposite sign input group-delay dispersion values for which SC pulses can be achieved systematically. In addition, we have observed that the energy coupled into the inner core of the filament is always of the order of 20% of the total input pulse energy, which opens the way to a scalable technique to obtain intense short pulses directly from the filamentation process.

Quantum-path signatures in attosecond helical beams driven by optical vortices

High-order harmonic generation (HHG) driven by beams carrying orbital angular momentum has been recently demonstrated as a unique process to generate spatio-temporal coherent extreme ultraviolet (XUV)/x-ray radiation with attosecond helical structure. We explore the details of the mapping of the driving vortex to its harmonic spectrum. In particular we show that the geometry of the harmonic vortices is complex, arising from the superposition of the contribution from the short and long quantum paths responsible of HHG. Transversal phase-matching and quantum path interferences provide an explanation of the dramatic changes in the XUV vortex structure generated at different relative positions of the target respect to the laser beam focus. Finally, we show how to take advantage of transversal phase-matching to select helical attosecond beams generated from short or long quantum paths, exhibiting positive or negative temporal chirp respectively.

Glucocorticoids effects on exocrine pancreatic secretion in caerulein-induced acute pancreatitis in the rat.

The present work reports on exocrine pancreatic secretion in control rats, adrenalectomized rats and hydrocortisone-treated (10 mg/Kg/d) rats during 7 days, under normal conditions and after induction of acute pancreatitis with caerulein (20 micrograms/Kg) by 4 subcutaneous injections at hourly intervals. Pancreatic secretion was seen to be affected by the procedure of adrenalectomy, which led to a marked reduction in the secretion of proteins and amylase with respect to control values. This was probably due to the decrease occurring in the zymogen granules in the acinar cells of the exocrine pancreas, a phenomenon which also led to a decrease in pancreatic weight observed in these animals. Treatment with hydrocortisone induced a decrease in the secretion of proteins and amylase, as well as an increase in pancreatic weight. This agrees with the accepted hypothesis that large amounts glucocorticoids stimulate the synthesis and storage of proteins in the exocrine pancreas, reducing the secretory phase. The administration of high doses of caerulein under these conditions led to acute pancreatitis in the three groups of animals. This was paralleled by a dramatic decrease in protein and amylase secretion and by severe interstitial edema of the pancreas and by increases in serum amylase values. In the case of the animals treated previously with hydrocortisone, the latter were tripled with respect to the control animals. The conclusion is offered that since the storage of enzyme proteins is governed by glucocorticoids, which furthermore increase the sensitivity of the acinar cells to stimulation by secretagogues, the administration of these substances during the development of pancreatic lesions such as acute pancreatitis is highly compromising to the organism.

Cholinergic pathways are involved in secretin and VIP release and the exocrine pancreatic response after intraduodenally perfused acetic and lactic acids in the rat.

The response of the exocrine pancreas to intraduodenal perfusion of acetic and lactic acids in normal and previously atropinized rats was studied. Secretin and vasoactive intestinal peptide (VIP) plasma levels in portal plasma were also measured. Intraduodenal perfusion of both acetic and lactic acids significantly stimulated flow rate (from 0.29 ± 0.03 μl/min to a maximum of 1.06 ± 0.08 μl/min after acetic and from 0.35 ± 0.05 μl/min to a maximum of 1.13 ± 0.12 μl/min after lactic acid perfusion) and protein output (from 11.16 ± 2.33 μg/min to a maximum of 35.1 ± 7.4 μg/min after acetic and from 8.98 ± 0.95 μg/min to a maximum of 22.5 ± 1.3 μg/min after lactic acid perfusion). Atropine treatment significantly inhibited pancreatic flow rate and protein output after acetic acid perfusion, but no inhibition of flow rate and a slight decrease in the protein output after lactic acid perfusion were seen. With respect to plasma peptide concentrations, significant increases in secretin and VIP levels were found after perfusion of both organic acids; atropine administration significantly decreased plasma secretin levels after acetic acid administration although it did not affect plasma VIP concentrations. By contrast, atropine significantly increased plasma secretin levels, but significantly lower values of plasma VIP concentrations were observed after lactic acid perfusion. Therefore, cholinergic mechanisms are involved in the release of secretin and VIP and different types of control of exocrine pancreatic secretion occur, depending on the features of the intraduodenal stimulant.

Effect of adenosine and adenosine agonists on amylase release from rat pancreatic lobules

The effects of adenosine on the amylase secretion from rat pancreatic lobules have been studied. Adenosine induces a dose-dependent stimulation on amylase release, which is maximal at a concentration of 10(-4) M. It has been observed a clear inhibition of this secretory action when atropine was added whereas no amylase release was seen in isolated acini after adenosine. The effect of adenosine is completely blocked by the adenosine receptors antagonist theophylline (10(-4) M), but not by dipyridamole (10(-3) M), a drug that inhibits the transport of adenosine into the cell. The increase of amylase secretion induced by adenosine is inhibited by either the relatively selective A1 receptor antagonist PD116,948 (10(-6) M) and by the A2 receptor antagonist PD115,199 (10(-6) M). Significant increases of amylase release are observed after the relatively selective A1 receptor agonist R-PIA (10(-5) M) and after the relatively selective A2 receptor agonist NECA (10(-4) M). Finally, the effect of R-PIA is not modified by coincubation with PD115,199 and the effect of NECA is not affected by coincubation with PD116,948. These results suggest that the action of adenosine is mediated through the release of acetylcholine and probably by the simultaneous occupation of both A1 and A2 adenosine receptors, whereas the intracellular action of adenosine could be discarded.

Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation.

The pancreatic ductal tree conveys enzymatic acinar products to the duodenum and secretes the fluid and ionic components of pancreatic juice. The physiology of pancreatic duct cells has been widely studied, but many questions are still unanswered concerning their mechanisms of ionic transport. Differences in the transport mechanisms operating in the ductal epithelium has been described both among different species and in the different regions of the ductal tree. In this review we summarize the methods developed to study pancreatic duct secretion both in vivo and in vitro, the different mechanisms of ionic transport that have been reported to date in the basolateral and luminal membranes of pancreatic ductal cells and the regulation of pancreatic duct secretion by nervous endocrine and paracrine influences.ResumenEl árbol ductal pancreático transporta las enzimas pancreáticas de origen acinar hasta el duodeno y secreta el componente hidroelectrolítico del jugo pancreático. Aunque existen numerosos estudios sobre la fisiología de las células ductulares pancreáticas todavía quedan muchas cuestiones sin resolver con respecto a los mecanismos de transporte iónico en estas células. Existen diferencias en los mecanismos de transporte iónico en el epitelio ductular, tanto entre las diferentes especies estudiadas como entre las distintas zonas del árbol ductal. En esta revisión se hace un resumen de los diferentes métodos aplicados al estudio de la secreción pancreática ductular, tanto in vivo como in vitro; se describen los distintos mecanismos de transporte iónico, tanto en la membrana basolateral como en la luminal, descritos hasta la fecha en las células ductulares; y, finalmente, se detallan los mecanismos de regulación, nerviosos, endocrinos y paracrinos de la secreción pancreática ductular.

Caerulein-induced acute pancreatitis in the rat. Pancreatic secretory response to cholecystokinin

The response of pancreatic exocrine secretion to cholecystokinin (CCK), has been studied in experimental acute pancreatitis induced in rats by supramaximal doses of caerulein. Several doses of caerulein were used (4, 20 and 40 micrograms/Kg) and each one was administered by four subcutaneous injections over 3 h at hourly intervals. Pancreatic juice was collected 9 h after the first injection. The caerulein-treated animals showed a statistically significant increase in serum amylase levels. Secretory activity of ductular cells remained unchanged in all the caerulein-treated animals, but total protein and amylase secretion decreased significantly at all the caerulein doses used, both in resting conditions and under stimulation with CCK (1.25 micrograms/Kg/h). Despite this the acinar cells of rats treated with the lowest dose of caerulein retained a certain degree of secretory function since amylase activity in pancreatic juice was greater than in other groups of rats treated with higher doses of caerulein. Moreover, the percentage of increase observed in total protein and amylase in response to CCK respect to basal secretion is similar to that of the untreated animals. At higher doses (20 and 40 micrograms/Kg) the secretory capacity in response to CCK was inhibited. Therefore CCK administration in slight acute pancreatitis could be used as a therapy since it favours the secretion of pancreatic enzymes at percentual levels similar to those of the controls.

Dynamics of the formation of bright solitary waves of Bose-Einstein condensates in optical lattices

We present a detailed description of the formation of bright solitary waves in optical lattices. To this end, we have considered a ring lattice geometry with large radius. In this case, the ring shape does not have a relevant effect in the local dynamics of the condensate, while offering a realistic setup to implement experiments with conditions usually not available with linear lattices (in particular, to study collisions). Our numerical results suggest that the condensate radiation is the relevant dissipative process in the relaxation towards a self-trapped solution. We show that the source of dissipation can be attributed to the presence of higher-order dispersion terms in the effective mass approach. In addition, we demonstrate that the stability of the solitary solutions is linked with particular values of the width of the wave packet in reciprocal space. Our study suggests that these critical widths for stability depend on the geometry of the energy band, but are independent of the condensate parameters (momentum, atom number, etc.). Finally, the nonsolitonic nature of the solitary waves is evidenced showing their instability under collisions.

Acinar cholecystokinin (CCK) receptors in the exocrine pancreas of the rat: effect of adrenalectomy.

The binding of [125I]BH-CCK-8 to membranes of acinar cells from rats at 6 and 21 days after adrenalectomy was studied. The optimum conditions of time and temperature were previously established as being 120 min and 30°C. Under these conditions, the membranes of the adrenalectomized animals of both groups (6 and 21 days) bound more radioligand than those from control rats. However, a qualitative study of the binding showed that the affinities of binding were much lower; in particular, the high affinity receptors had a Kd of 0.94 ± 0.33 nM in the controls and this was 14.9 ± 1.29 nM in the 6-day adrenalectomized animals, although the maximum binding capacity did not vary significantly. However, in the case of the low affinity receptors, there was a gradual increase in the maximum binding capacity as the time after adrenalectomy progressed: 717 ± 121, 1,987 ± 183, and 10,175 ± 862 fmol/mg for the control, 6-day, and 21-day adrenalectomized rats, respectively. In the latter situation, the high affinity receptors completely disappeared. These results, which coincide with a marked deficit in protein secretion already described in adrenalectomized rats, can be accounted for in terms of the possible negative cooperativity exerted by the low affinity receptors on the high affinity hormone-receptor complex, the protein secretion of the acinar cells normally mediated by the high affinity receptors becoming paralyzed.