J. T. Silva

Evaluation of mannan-oligosaccharides offered in milk replacers or calf starters and their effect on performance and rumen development of dairy calves

The objective of this study was to evaluate the route of administration of mannan-oligosaccharides in the diet of dairy calves and their effects on performance and plasma parameters indicative of rumen development. Following birth, twenty-four male Holstein calves were used in a completely randomized design and assigned to the following treatments: Control; 4 g/d Bio-Mos® (Alltech Biotech.) added to starter concentrate; and 4 g/d Bio-Mos® mixed into milk replacer. Animals were housed in individual hutches with free access to water, and fed 4L/d of milk replacer until weaning at six weeks. Calves also received 23g/kg crude protein of starter concentrate ad libitum. Fecal scores were evaluated daily. Body weights, growth measurements and blood samples for glucose, urea-N and β-hidroxibutyrate analyses were taken weekly until 8 weeks of age. There were no significant effects of treatment or treatment × age interactions for mean starter concentrate intake, weight gain or body growth. However, there was a significant age effect for all parameters. Fecal scores were not affected by treatments. Also, plasma concentration of glucose, urea-N or β-hidroxibutyrate were not affected by treatment or the treatment × age interaction. However, urea-N and β-hidroxibutyrate concentrations significantly increased with age, suggesting adequate rumen development. Under the conditions of this study, there were no calf performance benefits when mannan-oligosaccharides were incorporated into milk replacer or calf starter concentrate.

Intensive liquid feeding of dairy calves with a medium crude protein milk replacer: Effects on performance, rumen, and blood parameters

The objective of this study was to evaluate the effects of different liquid-feeding systems using a medium crude protein milk replacer on performance, rumen, and blood parameters. Thirty newborn Holstein calves were blocked according to birth weight and date of birth, and randomly distributed to different liquid-feeding systems: conventional (4 L/d), intensive (8 L/d), or step-up/step-down (wk 1, 4 L/d; wk 2 to 6, 8 L/d; wk 7 and 8, 4 L/d). The commercial milk replacer (12.5% solids, 20.2% crude protein, 15.6% fat) was fed twice daily (0700 and 1700 h) until calves were weaned, at 8 wk of age. Calves were individually housed in wood hutches, with free access to water and starter concentrate, and to hay only after weaning. They were followed through 10 wk of age. Milk replacer and starter intake were inversely affected by feeding system. After weaning, starter intake and hay intake were similar among feeding systems. Total dry matter intake was higher during the liquid-feeding period for calves on the intensive system compared to calves on the conventional system, but conventional feeding resulted in the highest dry matter intake after weaning. Feed efficiency was similar among feeding systems before and after weaning. Average body weight and daily gain were not affected by feeding system before or after weaning. During liquid feeding, diarrhea occurrence was lower for calves on the conventional system; however, when calves on the step-up/step-down system were fed lower volumes of liquid feed, diarrhea occurrence was similar to that of calves on the conventional system. Plasma concentrations of β-hydroxybutyrate were higher for calves on the conventional system, reflecting starter intake. Rumen pH, short-chain fatty acids, and N-NH3 were not affected by feeding system. Feeding higher volumes of milk replacer with a medium crude protein content had no beneficial effect on the performance of calves up to 10 wk of age.

Desempenho e parâmetros sanguíneos de bezerros leiteiros que receberam sucedâneo lácteo ou silagem de colostro

O objetivo deste estudo foi avaliar o desempenho e os parâmetros sanguineos de bezerros que consumiram colostro bovino fermentado sob condicoes anaerobias. Apos o nascimento, 18 bezerros da raca Holandes foram alojados em abrigos individuais e passaram a receber 4L da dieta liquida, sucedâneo lacteo ou silagem de colostro, divididos em duas refeicoes. O consumo de concentrado inicial e o escore fecal foram registrados diariamente, enquanto a pesagem e as colheitas de amostras de sangue para a determinacao das concentracoes plasmaticas de glicose, nitrogenio ureico, acidos graxos livres, β-hidroxibutirato e proteinas totais sericas foram realizadas semanalmente. Os animais alimentados com silagem de colostro apresentaram menores consumo de concentrado, ganho de peso diario e peso vivo. Todos os parâmetros sanguineos avaliados foram afetados pelos tratamentos, exceto a concentracao plasmatica de proteinas totais. O escore fecal foi afetado pelos tratamentos durante a segunda semana de vida, com animais alimentados com silagem de colostro apresentando fezes anormais e secas. O fornecimento de silagem de colostro como dieta liquida exclusiva nao resultou em desempenho animal adequado, nao sendo uma boa alternativa de substituto de leite.

Crude glycerin as a replacement for corn in starter feed: performance and metabolism of pre-weaned dairy calves

The purpose of this study was to evaluate the effect of partial replacement of corn in the starter feed with crude glycerin on performance and energy metabolism of liquid-fed dairy calves. Twenty-four newborn individually housed Holstein calves were blocked according to weight and date of birth and randomly assigned to different starter feed treatments during 8 weeks: no crude glycerin; 5% crude glycerin; 10% crude glycerin on dry matter basis. All calves were individually fed daily 4 L of a commercial milk replacer (22.3% crude protein and 19.3% fat). Calves had individual access to starter diet and ad libitum access to water daily. Individual feed intake and faecal scores were recorded daily. Bodyweight and corporal measurements (heart girth, hip width, and withers height) were weekly recorded. From the second week of age, blood samples were collected weekly, 2 h after morning feeding, for determination of plasma glucose and β-hydroxybutyrate concentrations. Ruminal fluid was collected at 4, 6 and 8 weeks of age for determination of pH and short-chain fatty acids. At the end of the eighth week, animals were harvested to evaluate development of the proximal digestive tract. Feed intake, average daily gain, bodyweight or body measurements were not affected (P > 0.05) by the inclusion of crude glycerin in the starter feed. Faecal scores indicated that most of the calves had mild diarrhoea, with no treatment effect. Total short-chain fatty acids and the concentration of individual acids, as well as blood parameters, were not affected (P > 0.05) by the feed treatments. The total forestomach weight, the average weight of each compartment, the maximum capacity of reticulum-rumen, and papillae number, height and width were not affected (P > 0.05). In conclusion, crude glycerin can be used as an alternative energy source, replacing corn up to 10% in the starter feed for liquid-fed dairy calves without affecting growth, rumen development or metabolism.

Evaluation of milk replacer supplemented with lysine and methionine in combination with glutamate and glutamine in dairy calves

ABSTRACT This study aimed to evaluate performance, gut health and metabolic changes in dairy calves receiving milk replacer supplemented with lysine and methionine with 0.6% or 1% AminoGut (10% Glutamate and 10% Glutamine). Forty-five Holstein calves were blocked and distributed in the treatments: (1) Control: no supplementation; (2) Lys, Met, AminoGut 0.6%; (3) Lys, Met, AminoGut 1%. Animals were individually housed, with free access to water and concentrate. Milk replacer (6 L/d) was fed until the eighth week of life. Feed intake and faecal score were monitored daily. Calves were weighted and measured weekly. Blood samples were collected every other week to determine metabolites. Supplementation did not affect the performance nor the metabolism of calves. There was no effect of supplementation for faecal score. An age effect was observed for most of the variables, mainly as a response of starter intake and consequent shift from a pre to a functional ruminant metabolism. The supplementation of the milk replacer with lysine and methionine in association with 0.6% or 1% AminoGut had no effect on performance, gut health nor metabolism of dairy calves. More important than the fine adjustment of amino acid requirements is the adoption of practices to improve starter intake.

Effects of immunocastration on the performance and meat quality traits of feedlot-finished Bos indicus (Nellore) cattle

This study aimed to evaluate the effects of immunocastration on the performance, testosterone concentration, carcass traits and meat quality of feedlot-finished Nellore cattle. Fifty-eight males (430 ± 36 kg bodyweight; 24 months old) were separated into three groups in a randomised complete design: surgically castration (n = 15), immunocastrated (anti-gonadotropin-releasing hormone (Bopriva®) vaccine, n = 21) and non-castrated (n = 22). The animals were housed in individual pens and fed ad libitum for 90 days with a common diet comprising 70% concentrate and 30% roughage (corn silage). The non-castrated animals had a greater average daily gain, feed efficiency, hot carcass weight and carcass yield than the surgically castrated and immunocastrated animals (P < 0.05). Surgically castrated cattle grew faster and more efficiently than those castrated immunologically (P < 0.05). The surgically castrated and immunocastrated animals had higher colour values L*, a* and b* and cooking loss than non-castrated animals (P < 0.05). In conclusion, immunocastration decreased cattle performance compared with surgically castrated or non-castrated animals. However, immunocastration resulted in better meat quality compared with non-castrated, making it an alternative to surgical castration with no negative effects on animal welfare.

Desempenho de bezerros leiteiros recebendo probiótico contendo Bacillus subtilis e Bacillus licheniformis

Twenty-four Holstein calves were utilized and received 4L/d of liquid diet consisting of commercial milk replacer, and had free access to water and starter concentrate. Weaning occurred at the 8th week of age. Animals were distributed in a randomized block design, in the following treatments: 1) Control - without supplementation with probiotic, 2) Supplementation: 2g/d of Bacillus subtilis and Bacillus licheniformis, via milk replacer. Every week calves were weighted and body measurements were taken. Blood samples were drawn weekly for determination of hematocrit, glucose, total protein, urea and albumin. Fecal samples were weekly collected for lactic acid bacteria, enterobacteria couting and fecal pH determination. Concentrate starter intake and fecal scores were monitored daily. Body weight, average daily gain, and corporal measurements were not affected by the supplementation of probiotic; however, presented a significant age effect. The fecal score, fecal pH and starter intake were not affected by probiotic supplementation. However, starter intake and fecal pH were affected by age due to calves’ natural growth. The acid lactic bacteria count was higher than the count of enterobacteria during the whole evaluation period. Only enterobacteria were affected by the age of animals, while acid lactic bacteria remained constant despite little variations. Blood parameters were also not affect by supplementation of probiotic, but all of them, except albumin concentrations, were influenced by age. The supplementation with probiotic containing Bacillus subtilis and Bacillus licheniformis via milk replacer presented no benefits in dairy calves performance or metabolism and did not reduced the occurrence of diarrhea.

Performance and metabolism of dairy calves fed starter feed containing citrus pulp as a replacement for corn

The aim of the present study was to evaluate the effect of replacing corn grain with citrus pulp (CSP) in the starter concentrate on performance and metabolism of dairy calves. Twenty-four individually housed Holstein calves were blocked according to birth date and weight and fed one of the following concentrates: (1) concentrate containing 64% corn and 0% CSP (0CSP); (2) concentrate containing 32% corn and 32% CSP (32CSP); and (3) concentrate containing 0% corn and 64% CSP (64CSP). Animals were fed 4 L of milk replacer daily (20% crude protein, 16% ether extract) divided in two meals (at 0700 hours and 1800 hours). The starter feed and water were provided ad libitum. Starter intake and faecal score were monitored daily. Bodyweight and measurements (withers height, hip width and heart girth) were recorded weekly. Blood samples were collected weekly, and ruminal fluid samples at 4, 6 and 8 weeks of age, 2 h after the morning feeding. After 8 weeks, animals were harvested to evaluate the development of the proximal digestive tract. Concentrate composition had no effect (P > 0.05) on concentrate intake, weight gain, bodyweight, body measurements, and faecal score. However, there was a lower concentration of ruminal butyrate (P < 0.05) in animals receiving 0CSP than in those fed the starter containing CSP. The 0CSP concentrate resulted in a lower (P < 0.05) total weight of the proximal digestive tract, weight of the reticulum–rumen and weight of the omasum than did the other concentrates. The length of the rumen papillae was the greatest (P < 0.05) in 32CSP-fed animals. However, papillae area in animals fed 32CSP concentrate was larger (P < 0.05) than that in 0CSP- and 64CSP-fed animals, with no difference observed between these latter two treatments. However, 0CSP-fed animals showed a higher proportion of abomasum than did the animals fed 32CSP and 64CSP concentrates. The replacement of 50% or 100% of corn by CSP in the starter feed of dairy calves did not affect performance and probably contributed to the better development of the proximal digestive tract.

Performance and metabolism of calves fed starter feed containing sugarcane molasses or glucose syrup as a replacement for corn.

The aim of this study was to evaluate the effect of replacing corn grain for sugar cane molasses (MO) or glucose syrup (GS) in the starter concentrate on performance and metabolism of dairy calves. Thirty-six individually housed Holstein male calves were blocked according to weight and date of birth and assigned to one of the starter feed treatments, during an 8 week study: i) starter containing 65% corn with no MO or GS (0MO); ii) starter containing 60% corn and 5% MO (5MO); iii) starter containing 55% corn and 10% MO (10MO); and iv) starter containing 60% corn and 5% GS (5GS). Animals received 4 L of milk replacer daily (20 crude protein, 16 ether extract, 12.5% solids), divided in two meals (0700 and 1700 h). Starter and water were provided ad libitum. Starter intake and fecal score were monitored daily until animals were eight weeks old. Body weight and measurements (withers height, hip width and heart girth) were measured weekly before the morning feeding. From the second week of age, blood samples were collected weekly, 2 h after the morning feeding, for glucose, β-hydroxybutyrate and lactate determination. Ruminal fluid was collected at 4, 6, and 8 weeks of age using an oro-ruminal probe and a suction pump for determination of pH and short-chain fatty acids (SCFA). At the end of the eighth week, animals were harvested to evaluate development of the proximal digestive tract. The composition of the starter did not affect (p>0.05) concentrate intake, weight gain, fecal score, blood parameters, and rumen development. However, treatment 5MO showed higher (p<0.05) total concentration of SCFAs, acetate and propionate than 0MO, and these treatments did not differ from 10MO and 5GS (p>0.05). Thus, it can be concluded that the replacement of corn by 5% or 10% sugar cane molasses or 5% GS on starter concentrate did not impact performance, however it has some positive effects on rumen fermentation which may be beneficial for calves with a developing rumen.