Jack Bech Nielsen
Novo Nordisk
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Publication
Featured researches published by Jack Bech Nielsen.
Journal of Biotechnology | 2008
Aubrey Jones; Michael Lamsa; Torben P. Frandsen; Tina Spendler; Paul Harris; Alan Sloma; Feng Xu; Jack Bech Nielsen; Joel Cherry
Directed evolution coupled with a high-throughput robotic screen was employed to broaden the industrial use of the maltogenic alpha-amylase Novamyl from Bacillus sp. TS-25. Wild-type Novamyl is currently used in the baking industry as an anti-staling agent in breads baked at neutral or near neutral pH. However, the enzyme is rapidly inactivated during the baking process of bread made with low pH recipes and Novamyl thus has very limited beneficial effect for this particular application. In an effort to improve the performance of Novamyl for low pH bread applications such as sourdough and rye, two error-prone PCR libraries were generated, expressed in Bacillus subtilis and screened for variants with improved thermal stability and activity under low pH conditions. Variants exhibiting improved performance were iteratively recombined using DNA shuffling to create two generations of libraries. Relative to wild-type Novamyl, a number of the resulting variants exhibited more than 10 degrees C increase in thermal stability at pH 4.5, one of which demonstrated substantial anti-staling properties in low pH breads.
Archive | 1995
Martin Schülein; Karen Margrethe Oxenboll; Lene Nonboe Andersen; Søren Flensted Lassen; Markus Sakari Kauppinen; Jack Bech Nielsen
Archive | 1998
Henrik Lund; Jack Bech Nielsen; Martin Schülein; B O Damgaard; Kim Vilbour Andersen
Archive | 1995
Lene Lange; Jack Bech Nielsen; Martin Schülein
Archive | 1995
Dorrit Anita Aaslyng; Jack Bech Nielsen; Lone Nielsen
Archive | 1995
Dorrit Anita Aaslyng; Jack Bech Nielsen; Nielsen Lone Kierstein
Archive | 1994
Jack Bech Nielsen
Archive | 2002
Tina Spendler; Jack Bech Nielsen
Archive | 1998
Henrik Lund; Jack Bech Nielsen; Martin Schülein; Bo Damgaard; Kim Vilbour Andersen
Archive | 1998
Mads Eskelund Bjørnvad; Mariko Hatakeyama; Martin Schülein; Jack Bech Nielsen