Jae Young Oh

Protective effect of polysaccharides from Celluclast-assisted extract of Hizikia fusiforme against hydrogen peroxide-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish

Polysaccharides were extracted by Celluclast-assisted hydrolysis and ethanol precipitation from Hizikia fusiforme (HFPS), and their in vitro antioxidant effects were evaluated in Vero cells. HFPS contains 63.56±0.32% sulfate polysaccharides, which comprise fucose (53.53%), galactose (23.15%), glucose (5.95%), and xylose (17.37%). HFPS scavenged DPPH, alkyl, and hydroxyl radicals at IC50 values of 0.81±0.02, 0.25±0.02, and 0.21±0.03mg/mL, respectively. HFPS significantly reduced H2O2-induced cytotoxicity in Vero cells. Furthermore, HFPS reduced intracellular ROS levels, and H2O2-induced apoptosis. HFPS has a strong protective effect against H2O2-stimulated oxidative stress in vivo in zebrafish, and showed improved survival rate, decreased heart rate, and reduced ROS generation and cell death. These results suggest that HFPS may be useful as an antioxidant in medical and cosmetic industries.

Anti-inflammatory Effects of Galactose-Taurine Sodium Salt: A Taurine Derivate in Zebrafish In Vivo Model

Taurine, the plentiful amino acids in mammalian cells exerts various biological activities including antioxidant and anti-inflammatory effects. Inflammation can cause several diseases such as cancer, heart disease, rheumatoid arthritis and immune system reactions. Here, we investigated anti-inflammatory effects of Galactose-Taurine sodium salt (Gal-Tau), a newly synthesized taurine derivate in LPS-stimulated zebrafish embryos in vivo model. The result showed that Gal-Tau improved the survival rate and the edema in LPS-treated zebrafish embryos. Also, Gal-Tau effectively reduced the productions of nitric oxide (NO), reactive oxygen species (ROS) and cell death induced by LPS in zebrafish embryos. In addition, Gal-Tau regulated the expression levels of inflammatory mediators such as inducible NOS (iNOS) and cycloxygenase 2 (COX-2) as well as IL-6 and TNF-α, inflammatory cytokines known as important key mediators of inflammation. Taken together, this study first indicates that Gal-Tau could be considered as an effective anti-inflammatory material with its anti-inflammatory activity.

Protective Effects of An Enzymatic Hydrolysate from Octopus ocellatus Meat against Hydrogen Peroxide-Induced Oxidative Stress in Chang Liver Cells and Zebrafish Embryo

Octopus ocellatus, a marine cephalopod distributed in the coast of South Korea, China, Japan and tropical sea, contains high amounts of taurine. In this study, an enzymatic hydrolysate obtained from O. ocellatus meat was evaluated for its antioxidant effects using a human liver cell line and zebrafish embryo model. Enzymatic hydrolysates of the O. ocellatus meat (OOM) were prepared using six different enzymes. Among the enzymatic hydrolysates, Alcalase hydrolysate of OOM (OOMAH) showed the highest scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals and hydrogen peroxide (H2O2). Moreover, it showed a high oxygen radical absorbance capacity (ORAC). OOMAH treatment effectively reduced the hydroxyl radical-induced DNA damage. OOMAH reduced the production of reactive oxygen species (ROS) in H2O2-treated hepatocytes without cytotoxicity. Furthermore, OOMAH improved the survival rate and reduced the intracellular ROS levels in H2O2-treated zebrafish embryos. Compositional analysis of amino acids indicated a high content of taurine in OOMAH. Current results suggest that OOMAH possesses antioxidant bioactivities and could provide protective effects against H2O2-induced oxidative stress. Therefore, OOMAH might be used as a potential resource of functional foods.

Anti-inflammatory Effects of Galactose-Taurine Sodium Salt in LPS-Activated RAW 264.7 Cells

In this study, we synthesized Galactose-Taurine sodium salt (G-T) as a functional food ingredient to enhance biological activities of taurine. Also, anti-inflammatory effects of G-T were investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. G-T found to reduce the generations of the LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) via down-regulating the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Also, G-T reduced the secretion of inflammatory cytokines including interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF-α) in LPS-treated RAW 264.7 cells. Finally, we identified that G-T inhibits the activation of nuclear factor-κB (NF-κB) and the phosphorylation of inhibitor κB (IκB)-α. From these results, this study first suggests that G-T could be considered as an effective anti-inflammatory agent.

Protective Effects of An Water Extracts Prepared from Loliolus beka Gray Meat Against H 2 O 2 -Induced Oxidative Stress in Chang Liver Cells and Zebrafish Embryo Model

In this study, we first evaluated protective effects of Loliolus beka in a human liver cell line and zebrafish embryo model with its anti-oxidant activity. First, we prepared the water extract from L. beka meat (LBMW) at room temperature for 24 h and revealed it consisted of a rich taurine. LBMW exhibited the scavenging effects against 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and hydrogen peroxide (H2O2) as well as the high value of oxygen radical absorbance capacity (ORAC). Also, the hydroxyl radical-induced DNA damage was dose-dependently reduced by the treatment of LBMW. In addition, LBMW showed no cytotoxicity and reduced the production of reactive oxygen species (ROS) in H2O2-treated hepatocytes. Moreover, LBMW regulated the expression of an anti-apoptotic molecule, Bcl-2 and the expression of pro-apoptotic molecules, Bax and PARP in H2O2-treated hepatocytes as well as the increment of antioxidant mediated-HO-1 and Nrf2 protein expression. In further study, LBMW improved the survival rate and decreased the production of ROS in H2O2-treated zebrafish embryo model. Therefore, our results suggest that Loliolus beka has protective effects against H2O2-induced oxidative stress and may be used as a potential source for functional foods.

Radio-Protective Effects of Octopus ocellatus Meat Consisted of a Plentiful Taurine Against Damages Caused by Gamma Ray Irradiation

Gamma ray irradiation causes immune suppressive responses by inducing oxidative stress such as reduction of cell viability and damages in immune cells. In this present study, we investigated whether Octopus ocellatus meet (OM) consisted of a plentiful taurine has protective effects against damages caused by oxidative stress in murine splenocytes. First of all, we prepared the aqueous extract from OM (OMA) and identified it contained a plentiful taurine content. The result also showed that OMA exhibited the antioxidant activity by scavenging DPPH and ABTS+ radicals and hydrogen peroxide. In addition, OMA improved the cell viability without cytotoxicity in gamma ray-irradiated murine splenocytes. Moreover, OMA significantly reduced the production of reactive oxygen species (ROS) in gamma ray-irradiated splenocytes. In further study, we identified that OMA protected zebrafish embryo via improving the reduced survival rate and decreasing the formation of deformity caused by the exposure of gamma ray irradiation. Also, OMA decreased the production of NO and ROS in gamma ray-irradiated zebrafish embryos as well as the induction of cell death. In these results, this study suggests that the consumption of taurine-rich foods, such as O. ocellatus, may be useful for the useful material for the protection against oxidative stress.