Jaewoo Yoon

Algisphaera agarilytica gen. nov., sp. nov., a novel representative of the class Phycisphaerae within the phylum Planctomycetes isolated from a marine alga

A Gram-negative, strictly aerobic, reddish-pink pigmented, motile, spherical, agar-degrading bacterium designated 06SJR6-2T was isolated from a green coloured marine alga (Cladophora sp.). Phylogenetic analyses based on the 16S rRNA gene sequence indicated that the new strain was affiliated with the class Phycisphaerae and shared 89.2xa0% sequence similarity with Phycisphaera mikurensis FYK2301M01T, which is the only validly named member of the class Phycisphaerae. The strain could be differentiated phenotypically from the recognised member of the class Phycisphaerae. The major fatty acids of strain 06SJR6-2T were identified as C16:0, C18:1ω9c and anteiso-C15:0. Amino acid analysis of cell wall hydrolysates indicated that the novel isolate did not contain diaminopimelic acid in the cell wall. The DNA G+C content of the strain was determined to be 63.0xa0mol% and the major respiratory quinone was identified as menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus in the family Phycisphaeraceae, for which the name Algisphaera agarilytica gen. nov., sp. nov. is proposed. The type strain of A. agarilytica is 06SJR6-2T (=KCTC 32482Txa0=xa0NBRC 109894T).

Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov.

Two aerobic, Gram-reaction-negative, golden-yellow pigmented and rod-shaped bacteria, designated strains A5Q-118(T) and A5Q-27, were isolated from an unidentified sea squirt that thrives in the coral reefs off the coast of Okinawa, Japan. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolates were affiliated with the family Flammeovirgaceae of the phylum Bacteroidetes. Strains A5Q-118(T) and A5Q-27 shared 100u200a% sequence similarity with each other and showed <92u200a% similarity with other cultivated members of the family Flammeovirgaceae. The novel isolates were phenotypically and physiologically different from strains described previously. The DNA G+C content was 35.5-36.2 mol%, MK-7 was the major menaquinone and iso-C(15u200a:u200a0) and C(16u200a:u200a1)ω5c were the major fatty acids. Based on the results of this polyphasic taxonomic study, it was concluded that strains A5Q-118(T) and A5Q-27 represent a novel species in a new genus of the family Flammeovirgaceae, for which the name Aureibacter tunicatorum gen. nov., sp. nov. is proposed. Proposal for designation of the Flammeovirgaceae fam. nov. is also presented. The type strain of Aureibacter tunicatorum is A5Q-118(T) (u200a=u200aKCTC 23232(T) u200a=u200aNBRC 107587(T)).

Tunicatimonas pelagia gen. nov., sp. nov., a novel representative of the family Flammeovirgaceae isolated from a sea anemone by the differential growth screening method

A Gram-negative, strictly aerobic, reddish-pink pigmented, non-motile, rod-shaped strain designated N5DB8-4T, was isolated from an orange-striped sea anemone Diadumene lineata by a differential growth screening method. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flammeovirgaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (89.1%) to Porifericola rhodea N5EA6-3A2BT. The strain could be differentiated phenotypically from recognized members of the family Flammeovirgaceae. The G+C content of the DNA is 52.6xa0mol%, the major respiratory quinone is menaquinone 7 (MK-7) and iso-C15:0, C16:1ω5c and iso-C15:1 G (the double-bond position indicated by capital letter is unknown) were the major fatty acids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain represents a novel taxon for which the name Tunicatimonas pelagia gen. nov., sp. nov. is proposed. The type strain of Tunicatimonas pelagia is N5DB8-4T (=KCTC 23473Txa0=xa0NBRC 107804T).

Rubidimonas crustatorum gen. nov., sp. nov., a novel member of the family Saprospiraceae isolated from a marine crustacean.

A strictly aerobic, Gram-negative, reddish-orange pigmented, non-motile and rod-shaped bacterium, designated AK17-053T was isolated from a marine crustacean (Squillidae) living on tidal flats on the coast of the Ariake Sea, Nagasaki, Japan. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate could be affiliated with the family Saprospiraceae of the phylum Bacteroidetes and that it showed highest sequence similarity (84%) with Lewinella marina MKG-38T. The strain could be differentiated phenotypically from recognized members of the family Saprospiraceae. The G+C content of DNA was 55.3xa0mol%, MK-7 was the major menaquinone and iso-C15:0 and C16:1ω7c were the major fatty acids. On the basis of polyphasic taxonomic studies, it was concluded that strain AK17-053T represents a new genus of the family Saprospiraceae. We propose the name Rubidimonas crustatorum gen. nov., sp. nov. for this strain; its type strain is AK17-053T (=xa0MBIC08356Txa0=xa0NBRC 107717T).

Citreitalea marina gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine red alga and emended description of the genus Gangjinia

A Gram-negative, strictly aerobic, pale-yellow pigmented, rod-shaped bacterium designated A4O-5T was isolated from a red coloured marine alga (Chondrus ocellatus Holmes). Phylogenetic analyses based on the 16S rRNA gene sequence indicated that the new strain was affiliated with the family Flavobacteriaceae and shared approximately 90xa0% sequence similarity with Leptobacterium flavescens YM3-0301T and Gangjinia marincola GJ16T. The strain could be differentiated phenotypically from the related members of the family Flavobacteriaceae. The major fatty acids of strain A4O-5T were identified as iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). A complex polar lipid profile was present consisting of phosphatidylethanolamine, an unidentified phospholipid, three unidentified amino lipids and an unidentified lipid. The DNA G+C content of the strain was determined to be 41.9xa0mol% and the major respiratory quinone was identified as menaquinone 6 (MK-6). From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus in the family Flavobacteriaceae, for which the name Citreitaleamarina gen. nov., sp. nov. is proposed. The type strain of C.marina is A4O-5T (=KCTC 32483T=NBRC 109918T).

Spongiimonas flava gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from an unidentified marine sponge

A Gram-negative, strictly aerobic, golden-yellow pigmented, non-motile, rod-shaped strain designated J36A-7T was isolated from an unidentified yellow-green coloured marine sponge. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (85.6xa0%) to Nonlabens dokdonensis DSW-6T. The strain could be differentiated phenotypically from recognized members of the family Flavobacteriaceae. The major fatty acids of strain J36A-7T were identified as iso-C16:0, iso-C17:0 3-OH and summed feature three as defined by the MIDI system (C16:1 ω7c and/or iso-C15:0 2-OH). The DNA G+C content was determined to be 31.2xa0mol%, the major respiratory quinone was identified as menaquinone 6 (MK-6) and a complex polar lipid profile was present consisting of phosphatidylethanolamine, two unidentified glycolipids, one unidentified aminolipid and one unidentified lipid. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Spongiimonas flava gen. nov., sp. nov. is proposed. The type strain of S. flava is J36A-7T (=KCTC 32176Txa0=xa0NBRC 109319T).