Jaime Restrepo

Experiencia clinica en el manejo de fracturas de pene en el Hospital Universitario del Valle (Cali-Colombia)

OBJETIVO: El objetivo principal del presente estudio fue describir las caracteristicas clinicas de los pacientes con diagnostico de fractura de pene en el Hospital Universitario del Valle (Cali, Colombia). METODOS: Se realizo un estudio descriptivo, en el que se revisaron todas las historias clinicas de los pacientes con diagnostico de fractura de pene entre enero de 2001 a diciembre de 2008 en el Hospital Universitario del Valle (HUV) en la ciudad de Cali. Se tuvieron en cuenta variables relacionadas con los antecedentes urologicos, la etiologia, el diagnostico de la fractura, el procedimiento quirurgico y el seguimiento. Se realizo un analisis univariado con el programa estadistico STATA v. 10.1. RESULTADOS: Se encontraron 18 casos de fractura de pene con un promedio de edad de 30 anos. 11 pacientes (61%) presentaron el episodio secundario a la relacion sexual. Se presento edema, dolor y chasquido mas frecuentemente. El diagnostico fue clinico en el 100% de los casos. El abordaje quirurgico se realizo con una incision circunferencial en la mayoria de los pacientes. El cuerpo cavernoso derecho fue el mas frecuentemente lesionado y en la mayoria de los casos la lesion fue corregida con una sutura absorbible de forma continua. Los pacientes tuvieron una hospitalizacion posquirurgica en promedio de 1.5 dias. CONCLUSIONES: La fractura de pene es una entidad cuyo diagnostico es clinico, debe realizarse de forma precoz, y la reparacion quirurgica es el tratamiento de eleccion para evitar complicaciones y permitir que el paciente retorne a su actividad sexual satisfactoria de manera temprana.

Encapsulation of the Piperine Present in Piper tuberculatum Species Using Multilamellar Vesicles and Determination of its Antioxidant Power

The piperine is an alkaloid present in the vegetal species pipilongo (Piper tuberculatum). Antioxidant properties are associated to this plant. It was encapsulated in multilamellar vesicles (MLV). For that, the validation of the extraction methodology through calibration curves of standard piperine by high resolution liquid chromatography (HPLC) was performed, using a column of C18, mobile phase MetOH: H2O (70:30) and detected by UV to 340nm with a fluency rate of 0.7 mL/ min. The lineal and non-lineal ranges were found between 1 and 300 ppm, with R2 of 0.995. During the extraction of the pipilongo fruit, the effect of two factors, solvents and ultrasound power, was studied, for the optimization of conditions and higher extraction of piperine in pipilongo. The maximum concentration of piperine (246.50 ppm) was found, using ethanol as extraction solvent. The piperine was isolated from its extract, using a solution of KOH with 10 % ethanol. It was purified and characterized by melting point, IR, GC-MS y RMN 1H. Once the extract was obtained, it was proceeded to the formation of liposomes. The phosphatidylcholine and cholesterol vesicles were prepared with extract and with piperine at different concentrations and hydrated with buffer pH 7.40. The average size of the particles formed, measured by dynamic light scattering (DLS), was in a range of 299.7–531.9 nm. For the study of antioxidant activity, a solution of DPPH 0.2 mM was prepared and a reaction was carried out with the extract and piperine liposomes and their corresponding free forms. The IC50 calculated for the extract and piperine were 2.8±0.2 and 21333±1499 ppm, respectively. These values of IC50 were compared against a standard of reference Quercetin, prepared under the same conditions. The test with DPPH showed that the released form possesses greater antioxidant activity while in encapsulation form, it attenuates and stabilizes this capacity.