Jakub Ondráček
Technical University of Crete
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Publication
Featured researches published by Jakub Ondráček.
Heritage Science | 2013
Jiří Smolík; Ludmila Mašková; Naděžda Zíková; Lucie Ondráčková; Jakub Ondráček
To analyse deposition of fine particulate matter (PM) on book surfaces we put twelve bunches of cellulose filters on a free shelf of the National Library in Prague, exposed them for three, six, nine, and twelve months to indoor air and analysed them after each period by Scanning Electron Microscopy (SEM) and Ion Chromatography (IC). Results showed that fine particles were deposited predominantly on the surface of the top filter but partly also on the surfaces of inner filters. It indicates fine particles penetrated between filters. The penetration and deposition of particles was also modelled as Brownian diffusion between two parallel filters. The model prediction demonstrated that fine particles penetrate between filters, with the depth of penetration limited by parallel diffusional deposition on filter surfaces. This is in qualitative agreement with SEM and IC investigations. The results show that beside the top part fine PM can deposit onto all available surfaces of books.
Aerosol Science and Technology | 2015
Naděžda Zíková; Jakub Ondráček; Vladimír Ždímal
We have developed a new, fully controlled filter testing device and have used it to measure size-resolved penetration through a typically used filtration media for (but not only) atmospheric aerosol sampling. Twenty membrane and fiber filter pieces (mixed cellulose ester filters, polytetrafluoroethylene filters, quartz fiber filters, glass fiber filters, and polycarbonate filters) of various manufacturers and filter codes were examined. High variability in the penetration curve shapes, most penetrating particle size (MPPS) (from 20 nm to 90 nm) and penetration maxima (from 0.001% to almost 100%) has been found. The dependence of pressure drop on face velocity generally agrees with theory, the comparison of penetration at various face velocities proved the theoretical equations being able to determine MPPS only partially correctly. Although the variability within an individual filter of the same code is not negligible, it is small compared to the differences between the various filter types. The results not only differed from the information provided by the manufacturers, but in many cases also provided information otherwise unavailable, although affecting the sampling and also the ability of comparison with theory. To have enough information for the proper choice of the filter for a given purpose, it would be necessary to have not only total penetration given from the DOP standard measurement, but the MPPS, penetration maximum value and pressure drop as well. Copyright 2015 American Association for Aerosol Research
Indoor and Built Environment | 2014
Bjarke Mølgaard; Jakub Ondráček; Petra Št´ávová; Lucie Džumbová; M Martin Barták; Tareq Hussein; Jiří Smolík
Since decades, indoor aerosol models have been introduced to understand the behaviour of indoor aerosols. However, studies about model validation in multi-zone form are very rare because of the lack of high quality and well-controlled measurements. We utilized state-of-the-art measurement and modelling approaches to validate the Multi-Compartment and Size-resolved Indoor Aerosol Model (MC-SIAM) inside a two-zone apartment with natural ventilation. According to the MC-SIAM simulations, the ventilation rates ranged from 0.06 to 0.31 h–1 during closed windows and it was as high as 2.1 h–1 when a window was open; compared to the tracer gases analysis results, the ventilation rate was as high as 0.26 h–1 (closed windows) and 1.7 h–1 (open window). The internal airflow predicted with the MC-SIAM ranged from 9.3 to 11 m–3 h (tracer gas analysis 7.6–15 m–3 h) when the door between the internal rooms was opened. The methods utilized in this study have proven that indoor aerosol models such as the MC-SIAM are valid to describe the behaviour of indoor aerosol particles inside multi-zone dwellings with the assumption of well-mixed indoor air inside each zone. The next step in indoor aerosol models development should include re-suspension and new particle formation processes.
Aerosol Science and Technology | 2015
P. Moravec; J. Smolik; Jakub Ondráček; Petr Vodička; Radek Fajgar
A method of the continual generation of lead or lead oxide nanoparticles for potential subsequent inhalation experiments with laboratory animals was investigated. We examined the thermal decomposition and oxidation of lead bis(2,2,6,6-tetramethyl-3,5-heptanedionate) in an externally heated tube reactor as well as the evaporation and condensation of metallic lead. The particle production dependence on experimental conditions was investigated using a scanning mobility particle sizer (SMPS), and the particle characteristics were studied using transmission electron microscopy (TEM), high-resolution TEM (HRTEM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), atomic absorption spectroscopy (AAS), elemental and organic carbon analysis (EC/OC), X-ray photoelectron spectrometry (XPS), and X-ray diffraction (XRD) methods. The evaporation/condensation was evaluated as being the most suitable for inhalation experiments due to its simplicity, high production rate, and the well-defined composition of the nanoparticles. Copyright 2015 American Association for Aerosol Research
Nanomaterials | 2018
Daniela Pelclova; Vladimir Zdimal; Jaroslav Schwarz; Stepanka Dvorackova; Martin Komarc; Jakub Ondráček; Martin Koštejn; Petr Kačer; Stepanka Vlckova; Zdenka Fenclova; Alexey Popov; Lucie Lischkova; Sergey Zakharov; Dhimiter Bello
Researchers in nanocomposite processing may inhale a variety of chemical agents, including nanoparticles. This study investigated airway oxidative stress status in the exhaled breath condensate (EBC). Nineteen employees (42.4 ± 11.4 y/o), working in nanocomposites research for 18.0 ± 10.3 years were examined pre-shift and post-shift on a random workday, together with nineteen controls (45.5 ± 11.7 y/o). Panels of oxidative stress biomarkers derived from lipids, nucleic acids, and proteins were analyzed in the EBC. Aerosol exposures were monitored during three major nanoparticle generation operations: smelting and welding (workshop 1) and nanocomposite machining (workshop 2) using a suite of real-time and integrated instruments. Mass concentrations during these operations were 0.120, 1.840, and 0.804 mg/m3, respectively. Median particle number concentrations were 4.8 × 104, 1.3 × 105, and 5.4 × 105 particles/cm3, respectively. Nanoparticles accounted for 95, 40, and 61%, respectively, with prevailing Fe and Mn. All markers of nucleic acid and protein oxidation, malondialdehyde, and aldehydes C6–C13 were elevated, already in the pre-shift samples relative to controls in both workshops. Significant post-shift elevations were documented in lipid oxidation markers. Significant associations were found between working in nanocomposite synthesis and EBC biomarkers. More research is needed to understand the contribution of nanoparticles from nanocomposite processing in inducing oxidative stress, relative to other co-exposures generated during welding, smelting, and secondary oxidation processes, in these workshops.
IOP Conference Series: Earth and Environmental Science | 2016
Jan Hovorka; Cecilia Leoni; Veronika Dočekalová; Jakub Ondráček; Naděžda Zíková
Atmospheric aerosol is an omnipresent component of the Earth atmosphere. Aerosol particle of diameters 1 μm defines ultrafine or coarse aerosol particles, respectively. Aerosol particle concentrations within the planetary boundary layer - PBL are measured at the ground level while their vertical profiles in the PBL are usually estimated by modelling. The aim of this study was to construct vertical concentration profiles of ultrafine and coarse aerosol particles from airborne and ground measurements conducted in an urban airshed. Airborne measurements were done by an unmanned airship, remotely controlled with GPS 10 Hz position tracking, and electrically powered with propulsion vectoring, which allows average cruising speed of 6 m.s-1. The airship carried three aerosol monitors and a temperature sensor. The monitors acquired 1 Hz data on mass concentration of coarse and number concentration of ultrafine particles. Four flight sequences were conducted on the 2nd of March 2014 above Plesna village, up-wind suburb of Ostrava in the Moravian-Silesian region of the Czech Republic. The region is a European air pollution hot-spot. Repeated flights were carried out in several height levels up to 570 m above ground level - a.g.l. Early morning flight revealed a temperature inversion in the PBL up to 70 m a.g.l. This lead to coarse particle concentrations of 50 μgm-3 below the inversion layer and 10 μgm-3 above it. Concurrently, air masses at 90-120 m a.g.l. were enriched with ultrafine particles up to 2.5x104 cm-3, which may indicate a fanning plume from a distant emission source with high emission height. During the course of the day, concentrations of ultrafine and coarse particle gradually decreased. Nevertheless, a sudden increase of ultrafine particle concentrations up to 3.7x104 cm-3 was registered at 400 m a.g.l. at noon and also after a lag of 20 min at the ground. This may indicate formation of new aerosol particles at higher altitudes, which are then transported downward by evolved convective mixing. Detailed information acquired by the airship measurements allow us to better understand processes resulting in the increase of aerosol particle concentrations at ground level in urban air.
Nanomaterials | 2018
Daniela Pelclova; Vladimir Zdimal; Martin Komarc; Stepanka Vlckova; Zdenka Fenclova; Jakub Ondráček; Jaroslav Schwarz; Martin Koštejn; Petr Kačer; Stepanka Dvorackova; Alexey Popov; Pavlina Klusackova; Sergey Zakharov; Dhimiter Bello
Thousands of researchers and workers worldwide are employed in nanocomposites manufacturing, yet little is known about their respiratory health. Aerosol exposures were characterized using real time and integrated instruments. Aerosol mass concentration ranged from 0.120 mg/m3 to 1.840 mg/m3 during nanocomposite machining processes; median particle number concentration ranged from 4.8 × 104 to 5.4 × 105 particles/cm3. The proportion of nanoparticles varied by process from 40 to 95%. Twenty employees, working in nanocomposite materials research were examined pre-shift and post-shift using spirometry and fractional exhaled nitric oxide (FeNO) in parallel with 21 controls. Pro-inflammatory leukotrienes (LT) type B4, C4, D4, and E4; tumor necrosis factor (TNF); interleukins; and anti-inflammatory lipoxins (LXA4 and LXB4) were analyzed in their exhaled breath condensate (EBC). Chronic bronchitis was present in 20% of researchers, but not in controls. A significant decrease in forced expiratory volume in 1 s (FEV1) and FEV1/forced vital capacity (FVC) was found in researchers post-shift (p ˂ 0.05). Post-shift EBC samples were higher for TNF (p ˂ 0.001), LTB4 (p ˂ 0.001), and LTE4 (p ˂ 0.01) compared with controls. Nanocomposites production was associated with LTB4 (p ˂ 0.001), LTE4 (p ˂ 0.05), and TNF (p ˂ 0.001), in addition to pre-shift LTD4 and LXB4 (both p ˂ 0.05). Spirometry documented minor, but significant, post-shift lung impairment. TNF and LTB4 were the most robust markers of biological effects. Proper ventilation and respiratory protection are required during nanocomposites processing.
Journal of Nutrition and Food Sciences | 2018
Milos Beran; Josef Drahorad; Ondrej Vltavsky; Marian Urban; Iva Laknerova; Martin Fronek; Jiri Sova; Jakub Ondráček; Lucie Ondráčková; Martina Kralova; Slavka Formankova
New Carbon Dioxide Assisted Spray Nebulisation Drying (CASND) technology has been used to produce microparticulated protein concentrates for human nutrition from alternative plant sources - hemp and canola seed filtration cakes. Alkali extraction was used to extract the proteins from the filtration cakes. The protein solutions after the alkali extractions were dried with the CASND demonstrator ATOMIZER. Aerosol particle size distribution and concentration in the draying chamber were determined by two different on-line aerosol spectrometers SMPS (Scaning Mobility Particle Sizer) and APS (Aerodynamic Particle Sizer). The protein powders were in form of hollow spheres with average particle diameter about 600 nm. The particles were characterized by the SEM method. The functional properties of the microparticulated protein concentrates were compared with the same protein concentrates dried by the convenctional spray drying process. The protein microparticulatin resulted in improved foaming and emulsifying properties and formation of long-term stable water dispersions. Gluten-free baguettes were prepared with the microparticulated protein concentrates as only protein source and evaluated by sensory analysis.
Environmental Science & Technology | 2017
Jaroslav Schwarz; Otakar Makeš; Jakub Ondráček; Michael Cusack; Nicholas Talbot; Petr Vodička; Lucie Kubelová; Vladimír Ždímal
To the best of our knowledge, this study represents the first observation of multiday persistence of an indoor aerosol transformation linked to a kitchen degreaser containing monoethanol amine (MEA). MEA remaining on the cleaned surfaces and on a wiping paper towel in a trash can was able to transform ammonium sulfate and ammonium nitrate into (MEA)2SO4 and (MEA)NO3. This influence persisted for at least 60 h despite a high average ventilation rate. The influence was observed using both offline (filters, impactors, and ion chromatography analysis) and online (compact time-of-flight aerosol mass spectrometer) techniques. Substitution of ammonia in ammonium salts was observed not only in aerosol but also in particles deposited on a filter before the release of MEA. The similar influence of other amines is expected based on literature data. This influence represents a new pathway for MEA exposure of people in an indoor environment. The stabilizing effect on indoor nitrate also causes higher indoor exposure to fine nitrates.
Atmospheric Environment | 2006
Tareq Hussein; Thodoros Glytsos; Jakub Ondráček; Pavla Dohányosová; Vladimír Ždímal; Kaarle Hämeri; Mihalis Lazaridis; Jiří Smolík; Markku Kulmala