James E. Carter

High-performance liquid chromatographic determination of sodium nitroprusside.

A rapid high-performance liquid chromatographic method for the determination of nitroprusside in commercial lyophilized products or in intravenous admixture solutions is described. The method is stability-indicating. Reversed-phase liquid chromatography was performed using a microparticulate (10 micrometer) phenyl column with a mobile phase acetonitrile--phosphate/tetrabutylammonium hydroxide buffer (pH 7.1) (30:70) and detection at 210 nm. A coefficient of variation of less than 3.1% was achieved over the concentration range studied (10--50 microgram/ml). Total analysis time was 9 min. This method was used to show that there is a small loss of nitroprusside due to photodegradation during intravenous infusion, even when the admixture container is wrapped in foil as recommended and used expeditiously.

In vitro and in vivo testing and correlation for oral controlled/ modified release dosage forms. report of the 2nd workshop held December 1988, Washington, DC. U.S.A.

This report is from the second workshop held in Washington, DC, on controlled/modified release dosage forms. The objectives of this second workshop were to determine the optimum information needed to characterize the drug entity and the drug dosage form, to explore the in vitro-in vivo relationship, so as to determine the criteria for establishing an in vitro-in vivo correlation, as well as the usefulness of in vitro data in the drug approval/regulatory process