James F. Stamps

Application of Microseparation Arrays to the Detection of Biotoxins in Aerosol Backgrounds

We are investigating the use of parallel separation channels that utilize orthogonal separation techniques as a platform for a biosensor to accurately identify protein biotoxins. Two separation techniques were initially chosen for proof of concept demonstration—capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE)—and have been implemented in our compact “µChemLab™” device. This device integrates automated high-voltage control of microchip-based separations with laser-induced fluorescence (LIF) detection and on-board data analysis. The effectiveness of this two-channel approach was evaluated using biotoxin-spiked aerosol samples.

Results from µChemLab™/CB, a Portable System for Detecting Chemical and Biological Warfare Agents

We recently tested µChemLab™ /CB, a hand-portable detection system developed for protein biotoxins, with biotoxin isoforms at Defense Science and Technology Laboratory (Dstl). We found the device is capable of detecting and discriminating various toxins and toxin isoforms.

Engineered Improvement of the Generation-2 μChemlab™ Biotoxin Detector

The μChemLab™ program is developing hand-portable systems for detecting a broad range of chemical, biological, and viral agents in both gas and liquid samples. The μChem Lab liquid sample analyzer employs electrokinetic sample injection, chip-based electrophoretic microseparations and laser-induced florescence detection to analyze liquid samples. A second-generation liquid phase prototype is described. The device incorporates improvements from technological advances and applied research experience. New features include a modular design that readily accommodates on-chip preconcentration and additional separation techniques. The redesign reduces hardware failures, minimizes downtime during component replacement, improves usability, and provides increased sensitivity. Improvements have been made without compromising previous system performance.