James J. Jakubow

Peripheral serotonin is an incomplete signal for eliciting satiety in sham-feeding rats.

Peripheral administration of serotonin [5-hydroxytryptamine (5-HT)] to rats equipped with gastric cannulae reduced their 30-min consumption of sweetened milk after overnight deprivation whether the cannulae were closed (real feeding) or open (sham feeding). The anorectic action of 5-HT (1.6, 4.0, and 10.0 mumol/kg, IP) in sham feeding was dose-related, rapid in onset, and persisted during the 30-min testing session. However, 5-HT failed to elicit resting--the terminal behavioral phase of satiety--in sham-feeding rats. Direct comparison of the effects of 4.0 mumol/kg 5-HT under both feeding conditions established that this dose promoted resting only when rats fed with their cannulae closed. The actions of 5-HT on feeding and resting were behaviorally selective because serotonergic treatment did not retard the beginning of feeding, alter sham drinking of water, or reduce investigation by food-deprived rats of a novel object in an open field. Together, the results suggest that 5-HT exerts separate actions to inhibit feeding and accelerate the process of satiation as marked by resting. However, peripheral 5-HT is inadequate as a signal for modulating satiety in the absence of postingestive stimuli.

Tolerance to amphetamine hypophagia: a microstructural analysis of licking behavior in the rat.

The development of tolerance to amphetamine-induced hypophagia was assessed by recording changes in lick parameters in rats given chronic administration of the drug (2 mg/kg) and access to sweetened milk. Although licking and milk intake gradually recovered, the volume of milk ingested per lick remained suppressed. Amphetamine had no effect on the interlick interval or the force per lick. In contrast, the drug caused a sustained increase in the number of lick bursts (defined by pause criteria of 0.5-2.0 s) and a decrease in the number of licks per burst (but only at pause criteria of 0.5 and 1.0 s). These results suggest that tolerant rats frequently interrupt licking, resulting in less efficient capture of milk.

Tolerance to amphetamine hypophagia: a real-time depiction of learning to suppress stereotyped movements in the rat.

To analyze how tolerance develops to amphetamine-induced hypophagia, the authors recorded real-time licking responses in rats given chronic injections of the drug and access to milk for 30 min. Initially, licking was greatly reduced and occurred only late in the session. The acquisition of tolerance was characterized by a decrease in the latency to initiate licking, a gradual increase in the number of licks, and a reorganization of the temporal licking pattern such that licks were distributed throughout the session, interspersed with pauses. On post-tolerance dose-response tests, licking was directly proportional to drug dose in some rats. The results support the view that tolerance to amphetamine hypophagia involves a behavioral adaptation to the motor effects of the drug.

The Cover-Stimulus Effect: Role of Similarity in Durations of the CS and Cover Cues

Pigeons were exposed to a random relation between a conditioned stimulus (CS) and an unconditioned stimulus (US) under the signaled random procedure. All US presentations that were unpaired with the CS were preceded by a second cue, a cover stimulus. Consistent with prior studies, the signaled random procedure supported responding during the CS; however, magnitude of this cover-stimulus effect varied with the duration of the CS relative to the cover-stimulus duration. Higher levels of responding were maintained when the CS and cover stimulus were equal versus unequal in duration. Experiment 1 provides a systematic replication of a study by Williams (1994), who used rat subjects. Higher levels of CS-directed responding were maintained when the cover stimulus was equal in duration to the CS versus when it was shorter. Experiment 2 provides the first demonstration of the effects of cover-stimulus durations that are both longer and shorter than the CS duration, compared to a procedure in which the durations are equivalent. In support of a stimulus-duration-equality effect, level of responding was higher for all equal stimulus-duration groups in comparison to unequal stimulus-duration comparison groups. These effects were accommodated by a common-elements generalization approach to the Rescorla-Wagner model.

Pentylenetetrazol-induced seizures in pigeons and the effects of ethosuximide thereon

Previous research has shown that ethosuximide in high enough doses disrupts operant responding in pigeons. Whether or not these same doses protect against seizure activity in this species has not been determined. In the present study a system for scoring pentylenetetrazol-induced seizures in pigeons was developed and the effects of ethosuximide on such seizures were evaluated. Pentylenetetrazol at 15, 27 and 47 mg/kg reliably induced seizures in Experiment 1. In Experiment 2 six doses of ethosuximide were tested for their seizure-controlling effectiveness. Doses of 20, 40, 80, 160 and 320 mg/kg ethosuximide had little effect on seizures induced by 27 mg/kg pentylenetetrazol; 640 mg/kg significantly reduced but did not completely eliminate seizures. This dose (640 mg/kg) is several times higher than the doses found to disrupt operant behavior in our previous studies.