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Dive into the research topics where James P. Albarella is active.

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Featured researches published by James P. Albarella.


Journal of Immunological Methods | 1983

A colorimetric immunoassay based on an enzyme inhibitor method

Michael A. Place; Robert J. Carrico; Frances M. Yeager; James P. Albarella; Robert C. Boguslaski

A competitive binding immunoassay was developed using an enzyme inhibitor for labeling the analyte. Thyroxine was labeled by covalent coupling of the alpha-amino group to the gamma-carboxyl of the glutamyl residue of methotrexate. This thyroxine-methotrexate conjugate was a potent inhibitor of dihydrofolate reductase. When antibody was bound to the thyroxine moiety, the inhibitor was inactivated. Thus, a competitive binding immunoassay for thyroxine was demonstrated based on colorimetric measurement of dihydrofolate reductase activity.


Journal of Chromatography A | 1985

Use of high-performance liquid chromatography in the preparation of flavin adenine dinucleotide analyte conjugates

Kin Fai Yip; James P. Albarella

Abstract High-performance liquid chromatography (HPLC) was used for the study of the chemical synthesis of the flavin adenine dinucleotide analyte conjugates. A preparative HPLC method was also developed for the purification of the conjugates directly from the reaction mixture with minimum sample pretreatment. The preparations of flavin N6-(N-6-aminohexyl)adenine dinucleotide and flavin adenine dinucleotide theophylline conjugate are given as examples to illustrate the importance of this method.


Archive | 1984

Nucleic acid hybridization assay employing antibodies to intercalation complexes

James P. Albarella; Leslie H Anderson


Archive | 1982

Iodothyronine immunogens and antibodies

James P. Albarella; Robert J. Carrico; Thomas M. Li


Analytical Chemistry | 1981

Flavin adenine dinucleotide as a label in homogeneous colorimetric immunoassays

David L. Morris; Paul B. Ellis; Robert J. Carrico; Frances M. Yeager; Hartmut Schroeder; James P. Albarella; Robert C. Boguslaski; William Edward Hornby; Denise. Rawson


Archive | 1982

Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives

James P. Albarella


Archive | 1984

Hybridization assay employing labeled pairs of hybrid binding reagents

James P. Albarella; Deriemer Leslie H. Anderson; Robert J. Carrico


Molecular and Cellular Probes | 1987

A solution hybridization assay for ribosomal RNA from bacteria using biotinylated DNA probes and enzyme-labeled antibody to DNA:RNA

Clifford O. Yehle; William L. Patterson; Sophie J. Boguslawski; James P. Albarella; Kin Fai Yip; Robert J. Carrico


Archive | 1984

Hybridization assay with immobilization of hybrids by antihybrid binding.

James P. Albarella; Deriemer Leslie H. Anderson; Robert J. Carrico


Archive | 1981

N-Aminoalkyl iodothyronine derivatives

James P. Albarella; Robert J. Carrico; Thomas M. Li

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